Powell, Jessica: Gephyrocapsa huxleyi chlorophyll-a content per cell volume after marine heatwave laboratory experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995604 (dataset in review)
Abstract:
Cultures of Gephyrocapsa huxleyi isolated from the Subantarctic water (SAW) off the coast of New Zealand were exposed to a simulated marine heatwave, and thermal performance curve experiments were performed immediately after the heatwave (T1) and again five weeks later (T2). After each thermal performance curve experiment, the cells in a subsample of each replicate were counted. The number of G. huxleyi cells in 25µL of fresh culture was counted using an Accuri C6 plus (BD) flow cytometer set to a slow flow rate (14µL/min, 10µm core size). The diameters of G. huxleyi cells were measured, and the cell volume was calculated by modelling the cell as a sphere (Hillebrand et al., 1999). Additional subsamples of each replicate at the end of the thermal performance curve experiments were collected for in vitro chlorophyll-a measurements. Cells were filtered onto GF/C filters, and chlorophyll-a was extracted using 90% acetone for 24 hours (Welschmeyer, 1994). The chlorophyll-a concentration was measured before and after acidification with a Turner 10-AU fluorometer. Final chlorophyll-a measurements were normalised per cell volume. The data was collected in 2024 as part of a Master's thesis research project investigating the effect of marine heatwaves on subantarctic phytoplankton.
Keyword(s):
Supplement to:
Powell, Jessica; Armstrong, Evelyn; Law, Cliff S (submitted): Subantarctic phytoplankton response to simulated marine heatwave conditions. Scientific Reports
References:
Hillebrand, Helmut; Dürselen, Claus-Dieter; Kirschtel, David; Pollingher, U; Zohary, Tamar (1999): Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology, 35(2), 403-424, https://doi.org/10.1046/j.1529-8817.1999.3520403.x
Price, Neil M; Harrison, Gail I; Hering, Janet G; Hudson, Robert J; Nirel, Pascale M V; Palenik, Brian; Morel, François M M (1989): Preparation and Chemistry of the Artificial Algal Culture Medium Aquil. Biological Oceanography, 6(5-6), 443-461, https://doi.org/10.1080/01965581.1988.10749544
Thomas, William H; Scotten, Harold L; Bradshaw, John S (1963): Thermal gradient incubators for small aquatic organisms. Limnology and Oceanography, 8(3), 357-360, https://doi.org/10.4319/lo.1963.8.3.0357
Welschmeyer, N A (1994): Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology and Oceanography, 39(8), 1985-1992, https://doi.org/10.4319/lo.1994.39.8.1985
Coverage:
Median Latitude: -45.850402 * Median Longitude: 171.026709 * South-bound Latitude: -45.864803 * West-bound Longitude: 170.512418 * North-bound Latitude: -45.836000 * East-bound Longitude: 171.541000
Date/Time Start: 2023-12-18T02:00:00 * Date/Time End: 2024-09-28T01:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Powell, Jessica | |||
| 2 | Date/time start, experiment | Date/time start exp | Powell, Jessica | |||
| 3 | Date/time end, experiment | Date/time end exp | Powell, Jessica | |||
| 4 | Treatment: photoperiod | T:photoperiod | h | Powell, Jessica | Day/Night cycle [12h:12h] | |
| 5 | Treatment: light intensity | T:Io | µmol/m2/s | Powell, Jessica | ||
| 6 | Medium | Medium | Powell, Jessica | According to Price et al. (1989) | Aquil 19.4 | |
| 7 | Treatment: dissolved iron | T:Fe diss | nmol/l | Powell, Jessica | ||
| 8 | Experimental treatment | Exp treat | Powell, Jessica | |||
| 9 | Treatment: temperature | T:temp | °C | Powell, Jessica | According to Thomas et al. (1963) | In aluminium heat block |
| 10 | Treatment: run-off | T:run-off | Powell, Jessica | T1: immediately after heatwave incubation, T2: 5 weeks after heatwave incubation | ||
| 11 | Gephyrocapsa huxleyi | G. huxleyi | #/l | Powell, Jessica | Fluorometer, Turner Designs, 10-AU | In vitro chl-a extracted using 90% acetone for 24 hours (Welschmeyer, N.A. 1994). Chl-a measured before and after acidification |
| 12 | Cylindrotheca sp. | Cylindrotheca sp. | #/l | Powell, Jessica | Flow Cytometer, BD Sciences, BD Accuri C6 | Cells in 25µL counted on slow flow rate |
| 13 | Gephyrocapsa huxleyi, chlorophyll total, per cell | G. huxleyi chl tot/cell | pg/# | Powell, Jessica | ||
| 14 | Gephyrocapsa huxleyi, volume | G. huxleyi vol | µm3 | Powell, Jessica | Inverted microscope, Olympus, IX51; equipped with a digital microscope camera, Evident, LC35 | Cell volume modelled as a sphere (Hillebrand et al, 1999), preserved cells measured without liths |
| 15 | Gephyrocapsa huxleyi, chlorophyll total, per cell volume | G. huxleyi chl tot/cell vol | pg/µm3 | Powell, Jessica | Normalized | Chl-a normalised with cell volume |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1800 data points