Powell, Jessica: Cylindrotheca sp. chlorophyll-a content per cell volume after marine heatwave laboratory experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995603 (dataset in review)
Abstract:
Cultures of Cylindrotheca sp. isolated from the Subantarctic water (SAW) off the coast of New Zealand were exposed to a simulated marine heatwave and thermal performance curve experiments were performed immediately after the heatwave (T1) and again five weeks later (T2). After each thermal performance curve experiment, the cells in a subsample of each replicate were counted. Cylindrotheca sp. cells were counted after preserving in 0.96% Glutaraldehyde and mounting a subsample in a Phycotech phytoplankton counting chamber under an inverted Olympus IX51 microscope. Cell size was measured by mounting a subsample of preserved cells on a phytoplankton counting chamber (Phycotech) and using an Evident LC35 camera and LCMicro software connected to an inverted Olympus IX51 microscope. Two measurements of Cylindrotheca sp. cells were made: both the length and the width of the chlorophyll-containing cell centre. The volume of the cell-centres was calculated based on the geometric model of a prolate sphere as described in Hillebrand et al. (1999). Additional subsamples of each replicate at the end of the thermal performance curve experiments were collected for in vitro chlorophyll-a measurements. Cells were filtered onto GF/C filters, and chlorophyll-a was extracted using 90% acetone for 24 hours (Welschmeyer, 1994). The chlorophyll-a concentration was measured before and after acidification with a Turner 10-AU fluorometer. Final chlorophyll-a measurements were normalised per cell volume. The data was collected in 2024 as part of a Master's thesis research project investigating the effect of marine heatwaves on subantarctic phytoplankton.
Keyword(s):
Supplement to:
Powell, Jessica; Armstrong, Evelyn; Law, Cliff S (submitted): Subantarctic phytoplankton response to simulated marine heatwave conditions. Scientific Reports
References:
Hillebrand, Helmut; Dürselen, Claus-Dieter; Kirschtel, David; Pollingher, U; Zohary, Tamar (1999): Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology, 35(2), 403-424, https://doi.org/10.1046/j.1529-8817.1999.3520403.x
Price, Neil M; Harrison, Gail I; Hering, Janet G; Hudson, Robert J; Nirel, Pascale M V; Palenik, Brian; Morel, François M M (1989): Preparation and Chemistry of the Artificial Algal Culture Medium Aquil. Biological Oceanography, 6(5-6), 443-461, https://doi.org/10.1080/01965581.1988.10749544
Thomas, William H; Scotten, Harold L; Bradshaw, John S (1963): Thermal gradient incubators for small aquatic organisms. Limnology and Oceanography, 8(3), 357-360, https://doi.org/10.4319/lo.1963.8.3.0357
Welschmeyer, N A (1994): Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology and Oceanography, 39(8), 1985-1992, https://doi.org/10.4319/lo.1994.39.8.1985
Coverage:
Median Latitude: -45.850402 * Median Longitude: 171.026709 * South-bound Latitude: -45.864803 * West-bound Longitude: 170.512418 * North-bound Latitude: -45.836000 * East-bound Longitude: 171.541000
Date/Time Start: 2023-12-18T02:00:00 * Date/Time End: 2024-09-28T01:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Powell, Jessica | |||
| 2 | Date/time start, experiment | Date/time start exp | Powell, Jessica | |||
| 3 | Date/time end, experiment | Date/time end exp | Powell, Jessica | |||
| 4 | Sampling date/time, experiment | Date/time sampling exp | Powell, Jessica | |||
| 5 | Treatment: light:dark cycle | T:L:D | hh:hh | Powell, Jessica | ||
| 6 | Treatment: light intensity | T:Io | µmol/m2/s | Powell, Jessica | ||
| 7 | Medium | Medium | Powell, Jessica | According to Price et al. (1989) | ||
| 8 | Treatment: dissolved iron | T:Fe diss | nmol/l | Powell, Jessica | ||
| 9 | Experimental treatment | Exp treat | Powell, Jessica | |||
| 10 | Treatment: temperature | T:temp | °C | Powell, Jessica | According to Thomas et al. (1963) | In aluminium heat block |
| 11 | Treatment: time point, description | T:time point descr | Powell, Jessica | |||
| 12 | Chlorophyll a | Chl a | µg/l | Powell, Jessica | Fluorometer, Turner Designs, 10-AU | In vitro chl-a extracted using 90% acetone for 24 hours (Welschmeyer, N.A. 1994). Chl-a measured before and after acidification |
| 13 | Cylindrotheca sp. | Cylindrotheca sp. | #/l | Powell, Jessica | Flow Cytometer, BD Sciences, BD Accuri C6 | Cells in 25µl counted on slow flow rate |
| 14 | Cylindrotheca sp., chlorophyll total, per cell | Cylindrotheca sp. chl tot/cell | pg/# | Powell, Jessica | ||
| 15 | Cylindrotheca sp., volume | Cylindrotheca sp. vol | µm3 | Powell, Jessica | Inverted microscope, Olympus, IX51; equipped with a digital microscope camera, Evident, LC35 | Cell volume modelled as a sphere (Hillebrand et al, 1999), preserved cells measured without liths |
| 16 | Cylindrotheca sp., chlorophyll total, per cell volume | Cylindrotheca sp. chl tot/cell vol | pg/µm3 | Powell, Jessica | Normalized | Chl-a normalized with cell volume |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1916 data points