Powell, Jessica: Cylindrotheca sp. growth rates during thermal performance curve laboratory experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995595 (dataset in review)
Abstract:
Subantarctic water off the east coast of New Zealand was collected in December 2023. Half of the water collected was incubated under 12.2°C (Control), and the other half was exposed to a 14-day mixed species marine heatwave simulation (15.8°C). Three individual populations of Cylindrotheca sp. were isolated from each treatment. After six months of maintenance under 12.2°C, a thermal performance curve experiment was performed. Triplicate cultures of the Control and Heatwave populations were grown in 30mL polycarbonate tubes (Oakridge) in Aquil medium (Price et al., 1989). The tubes were incubated in aluminium heatblocks (Thomas et al., 1963), with circulating water baths (Julabo), cooling one end and heating the other to create a thermal gradient with 10 temperatures from 10-20°C. The block was lit from below at ~85 µmol(photons)/m²/s on a 12:12 light:dark cycle. To account for minor variation in light levels, the replicates were rotated through the block each day at the same temperature. Chlorophyll was measured daily using a Turner 10-AU fluorometer. Growth rates were calculated from the slope of the natural log of the chlorophyll a against time while the replicates were in the exponential growth phase. The data was collected and analysed in 2024 as part of a Master's research project investigating the impacts of marine heatwaves on subantarctic phytoplankton.
Keyword(s):
Supplement to:
Powell, Jessica; Armstrong, Evelyn; Law, Cliff S (submitted): Subantarctic phytoplankton response to simulated marine heatwave conditions. Scientific Reports
References:
Price, Neil M; Harrison, Gail I; Hering, Janet G; Hudson, Robert J; Nirel, Pascale M V; Palenik, Brian; Morel, François M M (1989): Preparation and Chemistry of the Artificial Algal Culture Medium Aquil. Biological Oceanography, 6(5-6), 443-461, https://doi.org/10.1080/01965581.1988.10749544
Thomas, William H; Scotten, Harold L; Bradshaw, John S (1963): Thermal gradient incubators for small aquatic organisms. Limnology and Oceanography, 8(3), 357-360, https://doi.org/10.4319/lo.1963.8.3.0357
Coverage:
Median Latitude: -45.850402 * Median Longitude: 171.026709 * South-bound Latitude: -45.864803 * West-bound Longitude: 170.512418 * North-bound Latitude: -45.836000 * East-bound Longitude: 171.541000
Date/Time Start: 2023-12-18T02:00:00 * Date/Time End: 2024-09-28T01:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Powell, Jessica | |||
| 2 | Date/time start, experiment | Date/time start exp | Powell, Jessica | |||
| 3 | Date/time end, experiment | Date/time end exp | Powell, Jessica | |||
| 4 | Sampling date/time, experiment | Date/time sampling exp | Powell, Jessica | |||
| 5 | Treatment: photoperiod | T:photoperiod | h | Powell, Jessica | Day/Night cycle [12h:12h] | |
| 6 | Treatment: light intensity | T:Io | µmol/m2/s | Powell, Jessica | ||
| 7 | Medium | Medium | Powell, Jessica | According to Price et al. (1989) | ||
| 8 | Treatment: dissolved iron | T:Fe diss | nmol/l | Powell, Jessica | ||
| 9 | Experimental treatment | Exp treat | Powell, Jessica | |||
| 10 | Treatment: temperature | T:temp | °C | Powell, Jessica | According to Thomas et al. (1963) | In aluminium heat block |
| 11 | Cylindrotheca sp., growth rate | Cylindrotheca sp. µ | 1/day | Powell, Jessica | Fluorometer, Turner Designs, 10-AU | Growth rate calculated from slope of the natural log of in vivo fluorescence vs time during exponential growth phase |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
660 data points