Powell, Jessica: Cell volume of Gephyrocapsa huxleyi after exposure to a marine heatwave laboratory experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995576 (dataset in review)
Abstract:
Cultures of Gephyrocapsa huxleyi were isolated from the Subantarctic water (SAW) off the coast of New Zealand in 2023 and exposed to a simulated marine heatwave. Thermal performance curve experiments were performed for G. huxleyi at two time points after exposure to the marine heatwave: T1: immediately after heatwave exposure, and T2: 5 weeks after heatwave exposure. At the end of the thermal performance curve experiments, 1mL of each replicate was preserved using 25% Glutaraldehyde to a final concentration of 0.96% and stored at 4°C. A subsample of preserved cells was mounted on a phytoplankton counting chamber (Phycotech), and cell size was measured using an Evident LC35 camera and LCMicro software connected to an inverted Olympus IX51 microscope. The diameters of G. huxleyi cells were measured, and the cell volume was calculated by modelling the cell as a sphere (Hillebrand et al., 1999). Only the cell body was measured, as the preservation process results in the coccoliths detaching from the cell. The data was collected in 2024 as part of a Master's research project investigating the impacts of marine heatwaves on subantarctic phytoplankton.
Keyword(s):
Supplement to:
Powell, Jessica; Armstrong, Evelyn; Law, Cliff S (submitted): Subantarctic phytoplankton response to simulated marine heatwave conditions. Scientific Reports
References:
Hillebrand, Helmut; Dürselen, Claus-Dieter; Kirschtel, David; Pollingher, U; Zohary, Tamar (1999): Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology, 35(2), 403-424, https://doi.org/10.1046/j.1529-8817.1999.3520403.x
Price, Neil M; Harrison, Gail I; Hering, Janet G; Hudson, Robert J; Nirel, Pascale M V; Palenik, Brian; Morel, François M M (1989): Preparation and Chemistry of the Artificial Algal Culture Medium Aquil. Biological Oceanography, 6(5-6), 443-461, https://doi.org/10.1080/01965581.1988.10749544
Thomas, William H; Scotten, Harold L; Bradshaw, John S (1963): Thermal gradient incubators for small aquatic organisms. Limnology and Oceanography, 8(3), 357-360, https://doi.org/10.4319/lo.1963.8.3.0357
Coverage:
Median Latitude: -45.850402 * Median Longitude: 171.026709 * South-bound Latitude: -45.864803 * West-bound Longitude: 170.512418 * North-bound Latitude: -45.836000 * East-bound Longitude: 171.541000
Date/Time Start: 2023-12-18T02:00:00 * Date/Time End: 2024-09-28T01:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Powell, Jessica | |||
| 2 | Date/time start, experiment | Date/time start exp | Powell, Jessica | |||
| 3 | Date/time end, experiment | Date/time end exp | Powell, Jessica | |||
| 4 | Treatment: photoperiod | T:photoperiod | h | Powell, Jessica | Day/Night cycle [12h:12h] | |
| 5 | Treatment: light intensity | T:Io | µmol/m2/s | Powell, Jessica | ||
| 6 | Medium | Medium | Powell, Jessica | According to Price et al. (1989) | Aquil 19.4 | |
| 7 | Treatment: dissolved iron | T:Fe diss | nmol/l | Powell, Jessica | ||
| 8 | Experimental treatment | Exp treat | Powell, Jessica | |||
| 9 | Treatment: temperature | T:temp | °C | Powell, Jessica | According to Thomas et al. (1963) | In aluminium heat block |
| 10 | Treatment: run-off | T:run-off | Powell, Jessica | T1: immediately after heatwave incubation, T2: 5 weeks after heatwave incubation | ||
| 11 | Gephyrocapsa huxleyi, diameter | G. huxleyi diam | µm | Powell, Jessica | Inverted microscope, Olympus, IX51; equipped with a digital microscope camera, Evident, LC35 | Diameter of preserved cells measured without liths (cell body only) |
| 12 | Gephyrocapsa huxleyi, volume | G. huxleyi vol | µm3 | Powell, Jessica | According to Hillebrand et al. (1999) | Cell volume modelled as a sphere |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1440 data points