Powell, Jessica: Chlorophyll-containing cell volume of Cylindrotheca sp. after exposure to a marine heatwave laboratory experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995575 (dataset in review)
Abstract:
Cultures of Cylindrotheca sp. were isolated from the Subantarctic water (SAW) off the coast of New Zealand in 2023 and exposed to a simulated marine heatwave. Thermal performance curve experiments were performed for Cylindrotheca sp. at two time points after exposure to a marine heatwave: T1: immediately after heatwave exposure, and T2: 5 weeks after heatwave exposure. At the end of the thermal performance curve experiments, 1mL of each replicate was preserved using 25% Glutaraldehyde to a final concentration of 0.96% and stored at 4°C. A subsample of preserved cells was mounted on a phytoplankton counting chamber (Phycotech), and cell size was measured using an Evident LC35 camera and LCMicro software connected to an inverted Olympus IX51 microscope. Two measurements were made for Cylindrotheca sp. cells: both the length and the width of the chlorophyll-containing cell centre. The volume of the cell-centres was calculated based on the geometric model of a prolate sphere as described in Hillebrand et al. (1999). The data was collected in 2024 as part of a Master's research project investigating the impacts of marine heatwaves on subantarctic phytoplankton.
Keyword(s):
Supplement to:
Powell, Jessica; Armstrong, Evelyn; Law, Cliff S (submitted): Subantarctic phytoplankton response to simulated marine heatwave conditions. Scientific Reports
References:
Hillebrand, Helmut; Dürselen, Claus-Dieter; Kirschtel, David; Pollingher, U; Zohary, Tamar (1999): Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology, 35(2), 403-424, https://doi.org/10.1046/j.1529-8817.1999.3520403.x
Price, Neil M; Harrison, Gail I; Hering, Janet G; Hudson, Robert J; Nirel, Pascale M V; Palenik, Brian; Morel, François M M (1989): Preparation and Chemistry of the Artificial Algal Culture Medium Aquil. Biological Oceanography, 6(5-6), 443-461, https://doi.org/10.1080/01965581.1988.10749544
Thomas, William H; Scotten, Harold L; Bradshaw, John S (1963): Thermal gradient incubators for small aquatic organisms. Limnology and Oceanography, 8(3), 357-360, https://doi.org/10.4319/lo.1963.8.3.0357
Coverage:
Median Latitude: -45.850402 * Median Longitude: 171.026709 * South-bound Latitude: -45.864803 * West-bound Longitude: 170.512418 * North-bound Latitude: -45.836000 * East-bound Longitude: 171.541000
Date/Time Start: 2023-12-18T02:00:00 * Date/Time End: 2024-09-28T01:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Powell, Jessica | |||
| 2 | Date/time start, experiment | Date/time start exp | Powell, Jessica | |||
| 3 | Date/time end, experiment | Date/time end exp | Powell, Jessica | |||
| 4 | Sampling date/time, experiment | Date/time sampling exp | Powell, Jessica | |||
| 5 | Treatment: light:dark cycle | T:L:D | hh:hh | Powell, Jessica | ||
| 6 | Treatment: light intensity | T:Io | µmol/m2/s | Powell, Jessica | ||
| 7 | Medium | Medium | Powell, Jessica | According to Price et al. (1989) | ||
| 8 | Treatment: dissolved iron | T:Fe diss | nmol/l | Powell, Jessica | ||
| 9 | Experimental treatment | Exp treat | Powell, Jessica | |||
| 10 | Treatment: temperature | T:temp | °C | Powell, Jessica | According to Thomas et al. (1963) | In aluminium heat block |
| 11 | Treatment: time point, description | T:time point descr | Powell, Jessica | |||
| 12 | Cylindrotheca sp., length | Cylindrotheca sp. l | µm | Powell, Jessica | Inverted microscope, Olympus, IX51; equipped with a digital microscope camera, Evident, LC35 | Length of chl-containing cell centre |
| 13 | Cylindrotheca sp., width | Cylindrotheca sp. w | µm | Powell, Jessica | According to Hillebrand et al. (1999) | Width of chl-containing cell centre |
| 14 | Cylindrotheca sp., volume | Cylindrotheca sp. vol | µm3 | Powell, Jessica | According to Hillebrand et al. (1999) | Cell volume modelled as a prolate sphere |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1680 data points