Thielecke, Antonia; Jeon, Heejin; Whitehouse, Martin J: Single cell carbon and nitrate uptake rates from a mesocosm study on silicic acid effects on Arctic phytoplankton in Kongsfjord, Svalbard [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995570 (dataset in review)
Abstract:
This data was collected from a mesocosm experiment to assess the influence of silicic acid on Arctic phytoplankton, incubating the spring bloom community of Kongsfjorden, Svalbard, for 3 weeks (May 4 - May 26) under a gradient of Si concentrations. Every 4 days we conducted stable isotope (13C, 15N, 30Si) primary productivity bioassays. The water sampled from each mesocosms was transferred into duplicate 2L clear polycarbonate bottles. Stable isotopes were added to one set of replicates. The other set was used as a control. 13C-sodium bicarbonate was added at a 10 % enrichment level, 15N-sodium nitrate was added as a fixed quantity of 0.5 µmol/L. 30Si-sodium metasilicate was added relative to the intended Si concentration in the mesocosms aiming for a 15% enrichment. The bottles were incubated for 24 h under simulated in situ conditions in the lab at 2°C with 80-90 µmol photons m-2 s-1 of light. On day 6, 14 and 22) subsamples for single cell uptake analysis were taken from the bottles after the 24 h incubation.The samples were fixed with 2 % EM-grade paraformaldehyde (PFA; EMS, USA) for 24 h in the dark at 4 °C, then filtered onto 2 μm pore size polycarbonate filters (ø 25 mm; Merck Millipore Ltd., Cork, Ireland) and washed three times with ~2-3 mL of phosphate-buffered saline (Merck Millipore Ltd., Cork, Ireland). Single-cell 15N and assimilation rates were determined by high resolution secondary ion mass spectrometry (HR-SIMS; IMS 1280, CAMECA, Gennevilliers, France) following (Halbach et al., 2025).
Keyword(s):
Related to:
Halbach, Laura; Kitzinger, Katharina; Hansen, Martin; Littmann, Sten; Benning, Liane G; Bradley, J A; Whitehouse, Martin J; Olofsson, Malin; Mourot, Rey; Tranter, Martyn; Kuypers, Marcel MM; Ellegaard-Jensen, Lea; Anesio, Alexandre M (2025): Single-cell imaging reveals efficient nutrient uptake and growth of microalgae darkening the Greenland Ice Sheet. Nature Communications, 16(1), 1521, https://doi.org/10.1038/s41467-025-56664-6
Project(s):
Funding:
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven (AWI), grant/award no. AWIPEV_0015: AWI land-based expedition grant
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven (AWI), grant/award no. AWIPEV_0027: AWI land-based expedition grant
Coverage:
Latitude: 78.928063 * Longitude: 11.935453
Date/Time Start: 2024-05-10T00:00:00 * Date/Time End: 2024-05-26T00:00:00
Event(s):
NyA24_meso * Latitude Start: 78.928063 * Longitude Start: 11.935453 * Latitude End: 78.928063 * Longitude End: 11.935453 * Date/Time Start: 2024-05-04T08:00:00 * Date/Time End: 2024-05-26T20:00:00 * Location Start: Kongsfjorden, Svalbard * Location End: Kongsfjorden, Svalbard * Campaign: NyA24 * Basis: Ny Ålesund Research Station * Method/Device: Mesocosm experiment (MESO)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Type of study | Study type | ||||
| 3 | DATE/TIME | Date/Time | Geocode | |||
| 4 | Experiment day | Exp day | day | |||
| 5 | Mesocosm label | Meso label | ||||
| 6 | Treatment: silica, dissolved, added | T: Si diss add | µmol/l | |||
| 7 | File name | File name | ||||
| 8 | Taxon/taxa | Taxa | ||||
| 9 | Method comment | Method comm | ||||
| 10 | Number of pixels | No pix | # | |||
| 11 | Area | Area | µm2 | |||
| 12 | Atom percent, 30Si | At perc 30Si | % | Secondary ion mass spectrometry (SIMS), Cameca, IMS1280-HR | ||
| 13 | Atom percent, 15N | At perc 15N | % | Secondary ion mass spectrometry (SIMS), Cameca, IMS1280-HR | ||
| 14 | Atom percent, 13C | At perc 13C | % | Secondary ion mass spectrometry (SIMS), Cameca, IMS1280-HR | ||
| 15 | Cell biovolume | Cell biovol | µm3 | |||
| 16 | Carbon, organic, particulate, per cell | POC/cell | pg/# | |||
| 17 | Carbon uptake rate, specific | C upt rate spec | 1/day | Secondary ion mass spectrometry (SIMS), Cameca, IMS1280-HR | ||
| 18 | Nitrate uptake rate, specific | NO3 upt rate spec | 1/day | Secondary ion mass spectrometry (SIMS), Cameca, IMS1280-HR | ||
| 19 | Carbon uptake, per cell | C upt/cell | pg/#/day | Secondary ion mass spectrometry (SIMS), Cameca, IMS1280-HR |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
24917 data points
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