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Powell, Jessica: Cylindrotheca sp. growth rates during a simulated marine heatwave laboratory experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995566 (dataset in review)

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Abstract:
Cultures of Cylindrotheca sp. were isolated from Subantarctic Water (SAW) off New Zealand's east coast in December 2023 and exposed to a simulated marine heatwave incubation. Three replicates were incubated under two treatments: the average summer temperature of the SAW (Control, 12.2°C) and the maximum anomaly measured at the sample site at the time of experimental design (Heatwave, +3.6°C; EU Copernicus Marine Service, 2023). The heatwave temperature was first increased by 0.6°C, followed by 3 days of 1°C increase per day to a 7-day constant period at 15.8°C. At the end of the 7 days, the temperature was decreased by 1°C for 3 days and finally returned to 12.2°C at the end of the incubation. Biomass accumulation was monitored daily by measuring the in vivo chlorophyll-a using a Turner 10-AU fluorometer. The daily growth rate of the cultures was calculated from the natural log of the chlorophyll-a measurements against time.
Keyword(s):
G. huxleyi; growth rates; Laboratory experiment; phytoplankton; subantarctic; thermal performance curve
Supplement to:
Powell, Jessica; Armstrong, Evelyn; Law, Cliff S (submitted): Subantarctic phytoplankton response to simulated marine heatwave conditions. Scientific Reports
References:
Price, Neil M; Harrison, Gail I; Hering, Janet G; Hudson, Robert J; Nirel, Pascale M V; Palenik, Brian; Morel, François M M (1989): Preparation and Chemistry of the Artificial Algal Culture Medium Aquil. Biological Oceanography, 6(5-6), 443-461, https://doi.org/10.1080/01965581.1988.10749544
Thomas, William H; Scotten, Harold L; Bradshaw, John S (1963): Thermal gradient incubators for small aquatic organisms. Limnology and Oceanography, 8(3), 357-360, https://doi.org/10.4319/lo.1963.8.3.0357
Coverage:
Median Latitude: -45.850402 * Median Longitude: 171.026709 * South-bound Latitude: -45.864803 * West-bound Longitude: 170.512418 * North-bound Latitude: -45.836000 * East-bound Longitude: 171.541000
Date/Time Start: 2023-12-18T02:00:00 * Date/Time End: 2024-09-28T01:00:00
Event(s):
New_Zealand_SAW * Latitude: -45.836000 * Longitude: 171.541000 * Date/Time: 2023-12-18T02:00:00 * Location: New Zealand * Method/Device: Water pump (PUMP)
New_Zealand_SAW_LabExp * Latitude: -45.864803 * Longitude: 170.512418 * Date/Time Start: 2023-12-19T23:00:00 * Date/Time End: 2024-09-28T01:00:00 * Method/Device: Laboratory experiment
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typePowell, Jessica
2Date/time start, experimentDate/time start expPowell, Jessica
3Date/time end, experimentDate/time end expPowell, Jessica
4Treatment: photoperiodT:photoperiodhPowell, JessicaDay/Night cycle [12h:12h]
5Treatment: light intensityT:Ioµmol/m2/sPowell, Jessica
6MediumMediumPowell, JessicaAccording to Price et al. (1989)Aquil 19.4
7Treatment: dissolved ironT:Fe dissnmol/lPowell, Jessica
8Experimental treatmentExp treatPowell, Jessica
9Treatment: temperatureT:temp°CPowell, JessicaIncubator
10Treatment: durationT:durationdaysPowell, Jessica
11Chlorophyll aChl aRFUPowell, JessicaFluorometer, Turner Designs, 10-AU
12Chlorophyll a, natural logarithmln Chl aPowell, Jessica
13Cylindrotheca sp., growth rateCylindrotheca sp. µ1/dayPowell, JessicaFluorometer, Turner Designs, 10-AUGrowth rate calculated from slope of the natural log of in vivo fluorescence vs time for each day
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1086 data points

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