Powell, Jessica: Gephyrocapsa huxleyi growth rates during Thermal performance curves after exposure to marine heatwave laboratory experiment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995564 (dataset in review)
Abstract:
Cultures of Gephyrocapsa huxleyi were isolated from the Subantarctic water off the east coast of New Zealand in December 2023, and exposed to a simulated marine heatwave. Thermal performance curves (TPC) were produced at two time points after the heatwave. The first time (T1) was immediately after the heatwave incubations, and the second (T2) was five weeks after the heatwave incubations. During the five weeks in between experiments, both treatments were held under constant control conditions (12.2°C). Triplicates of the Control and Heatwave populations were grown in 30mL polycarbonate tubes (Oakridge) in Aquil medium (Price et al., 1989). The tubes were incubated in aluminium heatblocks (Thomas et al., 1963), with circulating water baths (Julabo), cooling one end and heating the other to create a thermal gradient with 10 temperatures from 10-20°C. The block was lit from below at ~85 µmol(photons)/m²/s on a 12:12 light:dark cycle. To account for minor variation in light levels, the replicates were rotated through the block each day at the same temperature. Chlorophyll was measured daily using a Turner 10-AU fluorometer. Growth rates were calculated from the slope of the natural log of the chlorophyll a against time while the replicates were in the exponential growth phase. The data was collected and analysed in 2024 as part of a Master's research project investigating the impacts of marine heatwaves on subantarctic phytoplankton.
Keyword(s):
Supplement to:
Powell, Jessica; Armstrong, Evelyn; Law, Cliff S (submitted): Subantarctic phytoplankton response to simulated marine heatwave conditions. Scientific Reports
References:
Price, Neil M; Harrison, Gail I; Hering, Janet G; Hudson, Robert J; Nirel, Pascale M V; Palenik, Brian; Morel, François M M (1989): Preparation and Chemistry of the Artificial Algal Culture Medium Aquil. Biological Oceanography, 6(5-6), 443-461, https://doi.org/10.1080/01965581.1988.10749544
Thomas, William H; Scotten, Harold L; Bradshaw, John S (1963): Thermal gradient incubators for small aquatic organisms. Limnology and Oceanography, 8(3), 357-360, https://doi.org/10.4319/lo.1963.8.3.0357
Coverage:
Median Latitude: -45.850402 * Median Longitude: 171.026709 * South-bound Latitude: -45.864803 * West-bound Longitude: 170.512418 * North-bound Latitude: -45.836000 * East-bound Longitude: 171.541000
Date/Time Start: 2023-12-18T02:00:00 * Date/Time End: 2024-09-28T01:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Powell, Jessica | |||
| 2 | Date/time start, experiment | Date/time start exp | Powell, Jessica | |||
| 3 | Date/time end, experiment | Date/time end exp | Powell, Jessica | |||
| 4 | Treatment: photoperiod | T:photoperiod | h | Powell, Jessica | Day/Night cycle [12h:12h] | |
| 5 | Treatment: light intensity | T:Io | µmol/m2/s | Powell, Jessica | ||
| 6 | Medium | Medium | Powell, Jessica | According to Price et al. (1989) | Aquil 19.4 | |
| 7 | Treatment: dissolved iron | T:Fe diss | nmol/l | Powell, Jessica | ||
| 8 | Experimental treatment | Exp treat | Powell, Jessica | |||
| 9 | Treatment: temperature | T:temp | °C | Powell, Jessica | According to Thomas et al. (1963) | In aluminium heat block |
| 10 | Treatment: run-off | T:run-off | Powell, Jessica | T1: immediately after heatwave incubation, T2: 5 weeks after heatwave incubation | ||
| 11 | Growth rate | µ | 1/day | Powell, Jessica | Fluorometer, Turner Designs, 10-AU | Growth rate calculated from slope of the natural log of in vivo fluorescence vs time during exponential growth phase |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1320 data points