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Majeed, Asma; Latif, Muhammad; Naqvi, Syed Ali Asad; Qazi, Adnan A; Munawar, Shaukat H; Waqas, Yasir; Farooq, Zahid; Naz, Huma; Shakeel, Ramsha; Wadood, Fazal; Ramzan, Asman: Human health risk indices for Pb, Ni, and Cd in edible freshwater fish (Labeo rohita and Cirrhinus mrigala) from Head Panjnad, Punjab, Pakistan (2022) [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995401 (dataset in review)

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Abstract:
This dataset documents seasonal heavy metal bioaccumulation in two commercially important edible freshwater fish species, Labeo rohita (Rohu) and Cirrhinus mrigala (Mrigal carp), collected from Head Panjnad, Punjab, Pakistan (29.3464 °N, 71.0197 °E), during summer and winter seasons of 2022. Head Panjnad represents the confluence zone of the major Punjab rivers and forms an important hydrological transition between the upper and lower Indus Basin. Fish specimens were collected from multiple locations near the Panjnad headworks, and concentrations of lead (Pb), nickel (Ni), and cadmium (Cd) were quantified in liver, gill, and muscle tissues using acid digestion followed by Atomic Absorption Spectrometry (AAS). Metal concentrations are reported as mean ± standard deviation on a wet weight basis (mg/kg ww). Across both species and seasons, bioaccumulation followed the order liver > gill > muscle, while metal abundance followed the pattern Pb > Ni > Cd. Seasonal comparisons demonstrated higher concentrations during summer relative to winter. The dataset additionally includes human health risk assessment metrics based on fish muscle consumption, including intake exposure (Exp_in), Target Hazard Quotient (THQ), Total Target Hazard Quotient (TTHQ), and Carcinogenic Risk (CR), calculated using USEPA methodologies. TTHQ values exceeded unity for both species, indicating potential cumulative non-carcinogenic concern for regular consumers, whereas individual carcinogenic risk values remained within acceptable USEPA thresholds. The dataset provides structured environmental monitoring information relevant to aquatic ecotoxicology, freshwater contamination assessment, food safety evaluation, and long-term Indus Basin biomonitoring applications.
Keyword(s):
AAS; Bioaccumulation; cadmium; Carcinogenic risk; Cirrhinus mrigala; Freshwater fish; Head Panjnad; heavy metals; Indus basin; Labeo rohita; lead; Muscle; nickel; Pakistan; Seasonal variation; THQ; TTHQ
Supplement to:
Majeed, Asma; Latif, Muhammad; Naqvi, Syed Ali Asad; Qazi, Adnan A; Munawar, Shaukat H; Waqas, Yasir; Farooq, Zahid; Naz, Huma; Shakeel, Ramsha; Wadood, Fazal; Ramzan, Asman (in prep.): Heavy metal contamination in fish from Head Panjnad, Punjab, Pakistan and associated human health risk assessment.
Related to:
Fatima, Saeed; Iqbal, Khalid Javed; Atique, Usman; Javid, Arshad; Khan, Noor; Iqbal, Sonia; Majeed, Hamid; Azmat, Hamda; Khan, Bakhat Yawar Ali; Irfan, Irfan; Shahid, Muhammad Tausif; Afzal, Gulnaz (2020): Toxic trace metals assessment in selected organs of edible fish species, sediment and water in Head Punjnad, Punjab, Pakistan. Punjab University Journal of Zoology, 35(1), https://doi.org/10.17582/journal.pujz/2020.35.1.43.50
Tchounwou, Paul B; Yedjou, Clement G; Patlolla, Anita K; Sutton, Dwayne J (2012): Heavy metal toxicity and the environment. In: Luch, A (eds.), Molecular, Clinical and Environmental Toxicology, Experientia Supplementum, 101, Springer Basel, Basel, 133-164, https://doi.org/10.1007/978-3-7643-8340-4_6
Ullah, A K M Atique; Maksud, M A; Khan, S R; Lutfa, L N; Quraishi, Shamshad B (2017): Dietary intake of heavy metals from eight highly consumed species of cultured fish and possible human health risk implications in Bangladesh. Toxicology Reports, 4, 574-579, https://doi.org/10.1016/j.toxrep.2017.10.002
World Health Organization (2011): Guidelines for Drinking-water Quality, 4th Edition [web service]. World Health Organization (WHO), Geneva, Switzerland., https://www.who.int/publications/i/item/9789241548151
References:
Onuoha, Samuel C; Anelo, P C; Nkpaa, Kpobari W (2015): Human health risk assessment of heavy metals in snail (Archachatina marginata) from four contaminated regions in Rivers State, Nigeria. American Chemical Science Journal, 11(2), 1-8, https://doi.org/10.9734/ACSJ/2016/22163
United States Environmental Protection Agency (2011): Integrated Risk Information System (IRIS) [web service]. United States Environmental Protection Agency (US EPA), Washington DC, USA, https://www.epa.gov/iris
Documentation:
Majeed, Asma; Latif, Muhammad; Naqvi, Syed Ali Asad; Qazi, Adnan A; Munawar, Shaukat H; Waqas, Yasir; Farooq, Zahid; Naz, Huma; Shakeel, Ramsha; Wadood, Fazal; Ramzan, Asman (2026): README_HeavyMetals. README_HeavyMetals.txt
Coverage:
Latitude: 29.346400 * Longitude: 71.019700
Date/Time Start: 2022-08-01T00:00:00 * Date/Time End: 2022-12-31T00:00:00
Event(s):
PJ-HM-2022 * Latitude: 29.346400 * Longitude: 71.019700 * Date/Time Start: 2022-08-01T00:00:00 * Date/Time End: 2022-12-31T00:00:00 * Location: Head Panjnad, Punjab, Pakistan * Method/Device: Fish sampling (FISHS) * Comment: Cyprinid freshwater fish (Cirrhinus mrigala, Labeo rohita) were collected in August/September and November/December 2022 (exact date unknown)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventMajeed, AsmaOrigin of fish samples
2Date/Time of eventDate/TimeMajeed, AsmaOrigin of fish samples
3Date/Time of event 2Date/Time 2Majeed, AsmaOrigin of fish samples
4Location of eventLocationMajeed, AsmaOrigin of fish samples
5Latitude of eventLatitudeMajeed, AsmaOrigin of fish samples
6Longitude of eventLongitudeMajeed, AsmaOrigin of fish samples
7Species, unique identificationSpecies UIDMajeed, AsmaReference in WoRMS (WoRMS)Fish species
8Species, unique identification (URI)Species UID (URI)Majeed, AsmaReference in WoRMS (WoRMS)Fish species
9Species, unique identification (Semantic URI)Species UID (Semantic URI)Majeed, AsmaReference in WoRMS (WoRMS)Fish species
10ElementsElementsMajeed, AsmaHeavy metals: Pb = lead, Ni = nickel, Cd = cadmium
11ExposureExposureMajeed, AsmaCalculated as Intake exposure (Exp_in) = (heavy metal concentration in fish muscle (C) x fish ingestion rate (IR) x exposure frequency (EFs) x exposure duration (ED)) / (body weight (BW) x averaging tim (AT))Intake exposure (Exp_in)
12Target hazard quotientTHQMajeed, AsmaCalculated as target hazard quotient (THQ) = intake exposure (Exp_in) / reference dose (RfD)Target hazard quotient (THQ); dimensionless non-carcinogenic risk index per metal
13Target hazard quotientTHQMajeed, AsmaCalculated as total target hazard quotient (TTHQ) = sum of all metal target hazard quotients (THQ) = Σ ⁿ ᵢ ₌ ₁ THQᵢTotal target hazard quotient (TTHQ); sum of all metal THQs
14CommentCommentMajeed, AsmaComment on total target hazard quotient (TTHQ)
15Cancer riskCRMajeed, AsmaCalculated as carcinogenic risk (CR) = intake exposure (Exp_in) x cancer slope factor (SFc)Carcinogenic risk (CR); probability of excess lifetime cancer risk (dimensionless)
16Reference doseRfDmg/kg/dayMajeed, AsmaAccording to US EPA (2011)Reference dose (RfD)
17Cancer slope factorCSFmg/kg/dayMajeed, AsmaAccording to Onuoha et al. (2015)Cancer slope factor (SFc)
18Tissue typeTissue typeMajeed, AsmaTissue basis
19--Majeed, AsmaAccording to US EPA (2011), Region III, Pakistan defaultFish ingestion rate (IR)
20Exposure timeExp timedaysMajeed, AsmaSelected reference valueExposure frequency (EFs)
21Exposition timeExp timeaMajeed, AsmaSelected reference valueExposure duration (ED)
22MassMasskgMajeed, AsmaSelected reference valueBody weight
23Time in daysTime ddaysMajeed, AsmaCalculated as product of exposure duration and exposure frequency (= ED x EFs)Averaging time (= 70 years x 365 days)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Size:
86 data points

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