Citation:

PANGAEA.994608 (dataset in review)

Name: Growth rate of Paracentrotus lividus larvae under different temperature, pH and lithium conditions
License: https://creativecommons.org/licenses/by/4.0/
Keywords: carbonate system; growth rate; interactive effects; Lithium contamination; Microcosm experiments; Multiple drivers; Paracentrotus lividus; Performance curves; Temperature

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Abstract:

A four-day experiment was conducted in October 2022 to resolve performance curves for temperature, pH and lithium contamination and understand their combined effects on Paracentrotus lividus larval growth. Adults were collected at Cap d'Ail, France and transferred to the Marine Environment Laboratories of the International Atomic Energy Agency (IAEA) in Monaco, where fertilization and the subsequent aquarium experiments were carried out in closed borosilicate bottles. Larval body length was measured after the experiment from microscopic photographs of the larvae, and growth rate calculated by dividing the body length with days since fertilization. At the end of the experiment, lithium concentration was measured with optical emission spectrometry, pH on the total scale with a TRIS-calibrated electrode, total alkalinity with open cell potentiometric titration, salinity with a salinometer, and the carbonate system calculated using the seacarb package in R. Temperature was measured continuously and averaged. This dataset provides the above parameters.

Keyword(s):

carbonate system; growth rate; interactive effects; Lithium contamination; Microcosm experiments; Multiple drivers; Paracentrotus lividus; Performance curves; Temperature

Supplement to:

Jónsson, Einar Pétur; Suhareva, Natalija; Orani, Anna Maria; Piscoya, Edson; Oberhaensli, Francois; Fernandes, Joana Filipa; Vellojin, Jurleys Paola; Metian, Marc; Yusseppone, Maria Soledad; Mutalipassi, Mirko; Sdiri, Khalil; Helguera Pedraza, Yusmila; Li, Zhenzhen; Dupont, Sam (in review): From single to multiple drivers – combined effect of temperature, pH and lithium on the larvae of the sea urchin Paracentrotus lividus. Journal of Experimental Biology

Coverage:

Latitude: 43.719650 * Longitude: 7.404220

Date/Time Start: 2022-10-01T00:00:00 * Date/Time End: 2022-10-31T00:00:00

Event(s):

P_lividus_2022 (Adult Paracentrotus lividus were collected in October 2022 for further fertilization in the laboratory to cunduct experiments with the larvae) * Latitude: 43.719650 * Longitude: 7.404220 * Date/Time Start: 2022-10-01T00:00:00 * Date/Time End: 2022-10-31T00:00:00 * Location: Cap d'Ail, France * Method/Device: Sampling by snorkeling (SNORKELING)

Comment:

Funding:

* Fondation Prince Albert II de Monaco, https://www.fpa2.org/

* Ocean Acidification International Coordination Center, https://www.iaea.org/services/oa-icc

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Jónsson, Einar Pétur
2	Type of study	Study type		Jónsson, Einar Pétur	Laboratory microcosm experiment
3	Species, unique identification	Species UID		Jónsson, Einar Pétur		Species of which larvae were used in the experiments
4	Species, unique identification (URI)	Species UID (URI)		Jónsson, Einar Pétur		Species of which larvae were used in the experiments
5	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Jónsson, Einar Pétur		Species of which larvae were used in the experiments
6	Date/time start, experiment	Date/time start exp		Jónsson, Einar Pétur
7	Date/time end, experiment	Date/time end exp		Jónsson, Einar Pétur
8	Experiment	Exp		Jónsson, Einar Pétur
9	Experimental treatment	Exp treat		Jónsson, Einar Pétur
10	Treatment: pH	T:pH		Jónsson, Einar Pétur		pH targeted for that treament
11	Treatment: temperature	T:temp	°C	Jónsson, Einar Pétur		Temperature targeted for that treament
12	Treatment: Lithium	T: Li	mg/l	Jónsson, Einar Pétur		Lithium targeted for that treament
13	Individual ID	Ind ID		Jónsson, Einar Pétur
14	Body length	BL	µm	Jónsson, Einar Pétur	Microscope image measured	Larval body length
15	Growth rate	µ	µm/day	Jónsson, Einar Pétur		Larval growth rate
16	Temperature, water	Temp	°C	Jónsson, Einar Pétur	Thermometer; average temperature measured in water baths	Average temperature measured in the water baths
17	pH, total scale	pHT		Jónsson, Einar Pétur	pH probe, calibrated using TRIS buffer	in situ pH; used for total alkalinity and seacarb calculations
18	Lithium	Li	mg/l	Jónsson, Einar Pétur	Inductively coupled plasma optical emission spectrometry (ICP-OES)
19	pH, NBS scale	pH NBS		Jónsson, Einar Pétur	pH probe, calibrated using NBS buffers
20	Temperature, water	Temp	°C	Jónsson, Einar Pétur	Thermometer; measured in sample for carbonate chemistry	Temperature measured in the sample taken for carbonate chemistry
21	Alkalinity, total	AT	µmol/kg	Jónsson, Einar Pétur	Open cell potentiometric titration; first of two simultaneous measurements	First of two simultaneous measurements
22	Alkalinity, total	AT	µmol/kg	Jónsson, Einar Pétur	Open cell potentiometric titration; second of two simultaneous measurements	Second of two simultaneous measurements
23	Alkalinity, total	AT	µmol/kg	Jónsson, Einar Pétur	Open cell potentiometric titration; mean value of two measurements	Mean of two measurements
24	pH, total scale	pHT		Jónsson, Einar Pétur	pH probe, calibrated using TRIS buffer; first repeated measurement	First of two subsequent measurements
25	pH, total scale	pHT		Jónsson, Einar Pétur	pH probe, calibrated using TRIS buffer; second repeated measurement	Second of two subsequent measurements
26	pH, total scale	pHT		Jónsson, Einar Pétur	pH probe, calibrated using TRIS buffer; mean value of two measurements	Mean of two measurements
27	Salinity	Sal		Jónsson, Einar Pétur	Salinometer (SALINO)	Salinity in practical salinity units (PSU)
28	Pressure	Press	atm	Jónsson, Einar Pétur	derived	Experiment carried out at sea level
29	Carbon dioxide	CO2	µmol/kg	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations
30	Carbon dioxide, partial pressure	pCO2	µatm	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations	Standard pCO₂ - CO₂ partial pressure computed at in situ temperature and atmospheric pressure (?atm)
31	Fugacity of carbon dioxide	fCO2	µatm	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations	Standard fCO₂ - CO₂ fugacity computed at in situ temperature and atmospheric pressure (?atm)
32	Carbon dioxide, partial pressure, potential	pCO2 pot	µatm	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations	Potential pCO₂ - CO₂ partial pressure computed at potential temperature and atmospheric pressure (?atm)
33	Fugacity of carbon dioxide, potential	fCO2 pot	µatm	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations	Potential fCO₂ - CO₂ fugacity computed at potential temperature and atmospheric pressure (?atm)
34	Carbon dioxide, partial pressure, in situ	pCO2 in situ	µatm	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations	In situ pCO₂ - CO₂ partial pressure computed at in situ temperature and total pressure (atm + hydrostatic) (?atm)
35	Fugacity of carbon dioxide, in situ	fCO2 in situ	µatm	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations	In situ fCO₂ - CO₂ fugacity computed at in situ temperature and total pressure (atm + hydrostatic) (?atm)
36	Bicarbonate ion	[HCO3]-	µmol/kg	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations
37	Carbonate ion	[CO3]2-	µmol/kg	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations
38	Carbon, inorganic, dissolved	DIC	µmol/kg	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations
39	Alkalinity, total	AT	µmol/kg	Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations
40	Aragonite saturation state	Omega Arg		Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations	Omega aragonite - aragonite saturation state
41	Calcite saturation state	Omega Cal		Jónsson, Einar Pétur	Calculated with seacarb package in R, version 3.3.4; using flag 8 for calculations	Omega calcite - calcite saturation state

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

13034 data points

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