Steinberg, Ronny; Mischke, Antje-Marie; Buschbaum, Christian; Bischof, Kai: Oxygen-based metabolic measurements from controlled laboratory temperature experiments in non-native Vaucheria sp. turfs in the European Wadden Sea [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.993634 (dataset in review)
Abstract:
This dataset describes the physiological temperature response of the intertidal macroalga Vaucheria sp. collected from the Wadden Sea near Sylt, Germany. The dataset provides insights into the thermal sensitivity of respiration and photosynthesis in Vaucheria sp. and can be used to assess physiological limits and potential responses of intertidal Vaucheria sp. to warming in temperate coastal ecosystems. Samples were collected in September 2024 from the lower intertidal zone and cultivated for three weeks under controlled laboratory conditions (15 °C, ~100 µmol photons m⁻² s⁻¹, 12:12 h light:dark cycle) before experimentation. The experiment was conducted in October 2024 in climate chambers at six temperature treatments (16, 20, 24, 28, 32, 36 °C). Physiological responses were assessed after 54 h of incubation. Each temperature treatment included four biological replicates. Respiration and net photosynthesis were quantified as oxygen consumption and production using fibre-optic optodes under four irradiance levels (0, 100, 500, 900 µmol photons m⁻² s⁻¹). Oxygen rates were normalised to algal wet weight. Photosynthetic performance was assessed using pulse-amplitude modulated (PAM) fluorometry, including measurements of maximum quantum yield of PSII (Fv/Fm), effective quantum yield (ΦPSII), non-photochemical quenching (NPQ) and rapid light curves.
Coverage:
Latitude: 54.957830 * Longitude: 8.381070
Date/Time Start: 2024-09-21T00:00:00 * Date/Time End: 2024-09-21T00:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Sampling date/time, experiment | Date/time sampling exp | Steinberg, Ronny | |||
| 2 | Type of study | Study type | Steinberg, Ronny | |||
| 3 | Family | Family | Steinberg, Ronny | |||
| 4 | Species | Species | Steinberg, Ronny | |||
| 5 | Genus | Genus | Steinberg, Ronny | |||
| 6 | Species, unique identification (URI) | Species UID (URI) | Steinberg, Ronny | |||
| 7 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Steinberg, Ronny | |||
| 8 | Day of experiment | DOE | day | Steinberg, Ronny | Measurements of day 3,6,9,12,15 and 18 | |
| 9 | Treatment: temperature | T:temp | °C | Steinberg, Ronny | Experimental temperatures in the lab: 16°C, 20°C, 24°C, 28°C, 32°C, 36°C | |
| 10 | Radiation, photosynthetically active | PAR | µmol/m2/s | Steinberg, Ronny | Light Meter, LI-COR GmbH, Germany, Licor Li-250A [with LI-190R Quantum sensor] | Light intensity between 400-700 nm wavelength |
| 11 | Respiration rate, oxygen | Resp O2 | µmol/l/h | Steinberg, Ronny | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0, 100, 500, 900 µmol photons m-2 s-1, channel 1 |
| 12 | Respiration rate, oxygen | Resp O2 | µmol/l/h | Steinberg, Ronny | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0, 100, 500, 900 µmol photons m-2 s-1, channel 2 |
| 13 | Respiration rate, oxygen | Resp O2 | µmol/l/h | Steinberg, Ronny | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0, 100, 500, 900 µmol photons m-2 s-1, channel 3 |
| 14 | Respiration rate, oxygen | Resp O2 | µmol/l/h | Steinberg, Ronny | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0, 100, 500, 900 µmol photons m-2 s-1, channel 4 |
| 15 | Photosynthetic rate, oxygen, per wet mass | Photosynth O/wm | µmol/mg/h | Steinberg, Ronny | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0, 100, 500, 900 µmol photons m-2 s-1 within one hour per miligram wet weight, channel 1 |
| 16 | Photosynthetic rate, oxygen, per wet mass | Photosynth O/wm | µmol/mg/h | Steinberg, Ronny | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0, 100, 500, 900 µmol photons m-2 s-1 within one hour per miligram wet weight, channel 2 |
| 17 | Photosynthetic rate, oxygen, per wet mass | Photosynth O/wm | µmol/mg/h | Steinberg, Ronny | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0, 100, 500, 900 µmol photons m-2 s-1 within one hour per miligram wet weight, channel 3 |
| 18 | Photosynthetic rate, oxygen, per wet mass | Photosynth O/wm | µmol/mg/h | Steinberg, Ronny | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0, 100, 500, 900 µmol photons m-2 s-1 within one hour per miligram wet weight, channel 4 |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
408 data points