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Karnatz, Josefine; Barthelmeß, Theresa; Sabbaghzadeh, Bita; Engel, Anja (2026): Biochemical composition and phytoplankton community of the sea surface microlayer and underlying water during ELISABETH MANN BORGESE cruise EMB295 (CenBASE) Jul-Aug 2022 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.993580 (DOI registration in progress), In: Karnatz, J et al. (2026): Biochemical composition and phytoplankton community of the sea surface microlayer and underlying water during ELISABETH MANN BORGESE cruise EMB295 (CenBASE) in 2022 [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.993574

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Published: 2026-04-17

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Abstract:
This dataset comprises samples collected in the Eastern Gotland Basin (Central Baltic Sea) during the EMB295 CenBASE research cruise conducted from June 30th to July 19th 2022. Sea surface microlayer (SML) samples were collected using established methods, i.e. the glass plate and the Garrett screen. Reference samples from the underlying water (ULW) were collected at approximately 1 m depth using a manually deployed Niskin bottle. To characterize the biochemical composition of the SML and the ULW, water samples were collected twice daily for the analysis of total organic carbon (TOC), total amino acids (TAA), total combined carbohydrates (TCCHO), and surfactants (determined using phase-sensitive AC voltammetry). TOC was measured using a TOC analyser. High-performance anion-exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) was performed for carbohydrate analysis. Amino acids were determined using a high-performance liquid chromatography (HPLC). Chlorophyll a samples were taken from the Niskin bottles at 1m depth (ULW) and analysed using a fluorometer from Turner Designs (Turner TD 10-AU005). Abundance and community composition of nano- and picophytoplankton (<=20µm) were determined by flow cytometry. Sampling stations were subsequently classified based on the abundance of microphytoplankton (>20 µm) and nano/picophytoplankton (≤20 µm).
Keyword(s):
air-sea interface; amino acids; Aphanizomenon sp.; Baltic Sea; carbohydrates; Cyanobacteria; Nodularia spumigena; Phytoplankton bloom; sea surface microlayer; Surfactants; synechococcus
Supplement to:
Karnatz, Josefine (in review): Biochemial Characteristics of the Sea Surface Microlayer in the Central Baltic Sea and Potential Signatures of Cyanobacterial Blooms.
Funding:
German Research Foundation (DFG), grant/award no. 451574234: FOR 5267 - Biogeochemical processes and Air-sea exchange in the Sea-Surface microlayer (BASS)
Coverage:
Median Latitude: 57.349722 * Median Longitude: 19.849900 * South-bound Latitude: 57.191428 * West-bound Longitude: 19.251424 * North-bound Latitude: 57.450808 * East-bound Longitude: 20.176119
Date/Time Start: 2022-07-06T16:29:40 * Date/Time End: 2022-07-16T04:57:07
Minimum Elevation: -245.0 m * Maximum Elevation: -81.0 m
Event(s):
EMB295_12-3 * Latitude: 57.249708 * Longitude: 20.090314 * Date/Time: 2022-07-06T16:29:40 * Elevation: -245.0 m * Location: Baltic Sea * Campaign: EMB295 (CenBASE) * Basis: Elisabeth Mann Borgese * Method/Device: CTD/Rosette (CTD-RO)
EMB295_12-7 * Latitude: 57.264087 * Longitude: 20.097581 * Date/Time: 2022-07-07T04:34:53 * Elevation: -245.0 m * Location: Baltic Sea * Campaign: EMB295 (CenBASE) * Basis: Elisabeth Mann Borgese * Method/Device: CTD/Rosette (CTD-RO)
EMB295_12-11 * Latitude: 57.191251 * Longitude: 20.072834 * Date/Time: 2022-07-07T16:24:33 * Elevation: -237.0 m * Location: Baltic Sea * Campaign: EMB295 (CenBASE) * Basis: Elisabeth Mann Borgese * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventKarnatz, Josefine
2Date/Time of eventDate/TimeKarnatz, Josefine
3LATITUDELatitudeKarnatz, JosefineGeocode
4LONGITUDELongitudeKarnatz, JosefineGeocode
5ClassificationClassificationKarnatz, Josefinephytoplankton abundance >20µm
6ClassificationClassificationKarnatz, Josefinephytoplankton abundance <20µm
7SamplingSamplingKarnatz, Josefine
8Depth zoneDepth zoneKarnatz, JosefineCTD
9Chlorophyll aChl amg/m3Karnatz, JosefineFluorometer, Turner Designs, 10-AU
10Chlorophyll a, standard deviationChl a std dev±Karnatz, Josefine
11PicoplanktonPicopl#/mlKarnatz, JosefineFlow cytometryChla_rich
12NanoplanktonNanopl#/mlKarnatz, JosefineFlow cytometryChla_rich
13PicoplanktonPicopl#/mlKarnatz, JosefineFlow cytometryphycoerythrin_rich
14NanoplanktonNanopl#/mlKarnatz, JosefineFlow cytometryphycoerythrin_rich
15Phytoplankton, totalPhytopl tot#/mlKarnatz, JosefineFlow cytometryflowcyto_sum
16Aspartic acid and AsparagineAsXnmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
17Glutamine and Glutamic acidGlXnmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
18SerineSernmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
19GlycineGlynmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
20ThreonineThrnmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
21ArginineArgnmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
22AlanineAlanmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
23gamma-Aminobutyric acidg-ABAnmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
24TyrosineTyrnmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
25ValineValnmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
26IsoleucineIlenmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
27PhenylalaninePhenmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
28LeucineLeunmol/lKarnatz, JosefineHigh Performance Liquid Chromatography (HPLC)
29Amino acid, totalTAAnmol/lKarnatz, Josefine
30Amino acid, totalTAAnmol/lKarnatz, JosefineC
31FucoseFucnmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
32RhamnoseRhanmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
33GalactosamineGalNnmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
34ArabinoseAranmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
35GlucosamineGlcNnmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
36GalactoseGalnmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
37GlucoseGlcnmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
38Mannose/XyloseMan/Xylnmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
39Galacturonic acidGalAnmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
40Glucuronic acidGlcAnmol/lKarnatz, JosefineHigh Performance anion-exchange chromatography
41Carbohydrates, total combinedTCCHOnmol/lKarnatz, Josefine
42Carbohydrates, total combinedTCCHOnmol/lKarnatz, JosefineC
43SurfactantsSurfactmg-eq/lKarnatz, JosefineVoltammetry, Metrohm, 796 VA ComputraceTX-100
44Carbon, organic, totalTOCµmol/lKarnatz, JosefineTOC analyzer (Shimadzu)
45Carbon, organic, total, standard deviationTOC std dev±Karnatz, Josefine
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1188 data points

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