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Steinberg, Ronny; Mischke, Antje-Marie; Buschbaum, Christian; Bischof, Kai: Physiological measurements from controlled laboratory temperature experiments in non-native Vaucheria sp. turfs in the European Wadden Sea [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.993563 (dataset in review)

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Abstract:
This dataset describes the physiological temperature response of the intertidal macroalga Vaucheria sp. collected from the Wadden Sea near Sylt, Germany. The dataset provides insights into the thermal sensitivity of respiration and photosynthesis in Vaucheria sp. and can be used to assess physiological limits and potential responses of intertidal Vaucheria sp. to warming in temperate coastal ecosystems. Samples were collected in September 2024 from the lower intertidal zone and cultivated for three weeks under controlled laboratory conditions (15 °C, ~100 µmol photons m⁻² s⁻¹, 12:12 h light:dark cycle) before experimentation. The experiment was conducted in October 2024 in climate chambers at six temperature treatments (16, 20, 24, 28, 32, 36 °C). Physiological responses were assessed after 54 h of incubation. Each temperature treatment included four biological replicates. Respiration and net photosynthesis were quantified as oxygen consumption and production using fibre-optic optodes under four irradiance levels (0, 100, 500, 900 µmol photons m⁻² s⁻¹). Oxygen rates were normalised to algal wet weight. Photosynthetic performance was assessed using pulse-amplitude modulated (PAM) fluorometry, including measurements of maximum quantum yield of PSII (Fv/Fm), effective quantum yield (ΦPSII), non-photochemical quenching (NPQ) and rapid light curves.
Keyword(s):
Climate change; Photosynthesis; Primary production; tidal flats; turf algae; warming
Coverage:
Latitude: 54.957830 * Longitude: 8.381070
Date/Time Start: 2024-09-21T00:00:00 * Date/Time End: 2024-09-21T00:00:00
Event(s):
Sylt_light * Latitude: 54.957830 * Longitude: 8.381070 * Date/Time: 2024-09-21T00:00:00 * Location: Wadden Sea, East of Nothern Sylt, Germany * Method/Device: Sampling by hand (HAND)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Sampling date/time, experimentDate/time sampling expSteinberg, Ronny
2Type of studyStudy typeSteinberg, Ronny
3FamilyFamilySteinberg, Ronny
4SpeciesSpeciesSteinberg, Ronny
5GenusGenusSteinberg, Ronny
6Species, unique identificationSpecies UIDSteinberg, Ronny
7Day of experimentDOEdaySteinberg, RonnyMeasurements of day 3,6,9,12,15 and 18
8Treatment: temperatureT:temp°CSteinberg, Ronny
9Time in secondsTime secsSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMof measurement
10Radiation, photosynthetically activePARµmol/m2/sSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMLight intensity between 400-700 nm wavelength
11Electron transport rate, relativerETRµmol e/m2/sSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate A
12Electron transport rate, relativerETRµmol e/m2/sSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate B
13Electron transport rate, relativerETRµmol e/m2/sSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate C
14Electron transport rate, relativerETRµmol e/m2/sSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate D
15Effective quantum yield of photosystem IIY(II)Steinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate A
16Effective quantum yield of photosystem IIY(II)Steinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate B
17Effective quantum yield of photosystem IIY(II)Steinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate C
18Effective quantum yield of photosystem IIY(II)Steinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate D
19Non photochemical quenchingNPQSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate A
20Non photochemical quenchingNPQSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate B
21Non photochemical quenchingNPQSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate C
22Non photochemical quenchingNPQSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate D
23Maximum quantum yield of photosystem IIFv/FmSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate A
24Maximum quantum yield of photosystem IIFv/FmSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate B
25Maximum quantum yield of photosystem IIFv/FmSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate C
26Maximum quantum yield of photosystem IIFv/FmSteinberg, RonnyMicroscopy Chlorophyll Fluorometer Heinz Walz GmbH, Germany, MICROSCOPY-PAMReplicate D
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1302 data points

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