Willmer, Joy; Schubert, Hendrik; Hölzel, Maj-Britt; Stegmann, Niklas; Kammann, Ulrike K R; Scharsack, Jörn Peter: Observational dataset of behavioral response of three-spined sticklebacks to TNT exposure in a closed laboratory setup [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.990263 (dataset in review)
Abstract:
Dumped munition in the German North Sea and Baltic Sea pose environmental risks as corrosion of the munition shells results in the leakage of the explosive 2,4,6-trinitroluene (TNT) into the marine environment. Uptake of TNT by marine biota and the associated negative effects on organisms are of major concern. This dataset reports behavioral responses of three-spined stickleback (Gasterosteus aculeatus) to environmentally relevant concentrations of TNT. Experimental sticklebacks were laboratory-bred and held in groups of 30 individuals in 60 L tanks in the fish facilities at the Thünen Institute of Fisheries Ecology in Bremerhaven. Parental sticklebacks originated from the Weser estuary (Luneplate, Bremerhaven, Germany, 53°28'36.9" N; 8°31'08.9" E) and were collected in April 2023. A total of 60 sticklebacks were tested in a controlled laboratory setup at the fish facilities at the Thünen Institute in Bremerhaven, containing two hideout zones formed by artificial plants. Each hideout was connected to an infusion system delivering either a TNT solution (100 µg/L) or control water into the zone currently occupied by the fish. Experimental trials were video-recorded to enable post hoc behavioral analysis. Behavioral metrics included the total time spent in the exposed hideout zone (s), latency to first leave the exposed hideout zone (s), and the number of crossings between hideout zones.
Keyword(s):
Supplement to:
Willmer, Joy; Schubert, Hendrik; Hölzel, Maj-Britt; Stegmann, Niklas; Kammann, Ulrike K R; Scharsack, Jörn Peter (in review): Three-spined sticklebacks do not avoid environmentally relevant concentrations of the toxic explosive 2,4,6-trinitrotoluene.
Project(s):
Funding:
Federal Ministry of Research, Technology and Space (BMFTR), grant/award no. 03F0981F: DAM sustainMare - CONMAR-2, Thünen
Coverage:
Latitude: 53.476920 * Longitude: 8.519140
Date/Time Start: 2023-04-05T00:00:00 * Date/Time End: 2023-04-05T00:00:00
Event(s):
Comment:
The total duration of the experiment was 12 days including two experimental trials:
* first trial: days 1-8, conducted by Hendrik Schubert
* second trial: days 9-12 , conducted by Joy Willmer
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Willmer, Joy | |||
| 2 | Experiment number | Exp no | Willmer, Joy | |||
| 3 | Experiment day | Exp day | day | Willmer, Joy | ||
| 4 | Date/time start, experiment | Date/time start exp | Willmer, Joy | UTC | ||
| 5 | Specimen identification | Spec ID | Willmer, Joy | Fish identification | ||
| 6 | Tank number | Tank No | Willmer, Joy | |||
| 7 | Gasterosteus aculeatus, total length | G. aculeatus TL | mm | Willmer, Joy | Fish measuring board | |
| 8 | Gasterosteus aculeatus, mass | G. aculeatus m | g | Willmer, Joy | Precision balance, KERN, EW 2200-2NM | |
| 9 | Gasterosteus aculeatus, sex | G. aculeatus sex | Willmer, Joy | Visual identification (VID) | ||
| 10 | Treatment | Treat | Willmer, Joy | |||
| 11 | Treatment code | Treat code | Willmer, Joy | |||
| 12 | Time of treatment start | Time treat start | s | Willmer, Joy | Smartphone application, Samsung, Stopwatch app [Samsung Galaxy A55] | Time of perfusion start |
| 13 | Gasterosteus aculeatus, time in hideout zone, total | G. aculeatus time hideout zone tot | s | Willmer, Joy | Smartphone application, Samsung, Stopwatch app [Samsung Galaxy A55] | Hideout 1 (determined during video analysis) |
| 14 | Gasterosteus aculeatus, time in hideout zone, total | G. aculeatus time hideout zone tot | s | Willmer, Joy | Smartphone application, Samsung, Stopwatch app [Samsung Galaxy A55] | Hideout 2 (determined during video analysis) |
| 15 | Number of crossings | No crossings | # | Willmer, Joy | Counted from video footage | |
| 16 | Gasterosteus aculeatus, time in hideout zone | G. aculeatus time hideout zone | s | Willmer, Joy | Smartphone application, Samsung, Stopwatch app [Samsung Galaxy A55] | Time until hideout 1 was first left (determined during video analysis) |
| 17 | Comment | Comment | Willmer, Joy |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2037 data points
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