Riedel, Elisabeth K; Lender, Laura: Life-history traits, development time and dry weight upon eclosion of Drosophila melanogaster under experimental variation of fly-symbionts and plant ontogenetic environment [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.989984 (dataset in review), In: Riedel, Elisabeth K; Lender, Laura; Pollierer, Melanie M; Rohlfs, Marko: Life-history trait and δ¹³C values of Drosophila melanogaster from experiments combining semi-natural fruit substrates with different fly fecal microbiota, collected from a microcosm set up [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.989940 (dataset in review)
Abstract:
In an experimental approach fruit and vegetable puree was inoculated with allochthonous and autochthonous fly fecal microbiota to record life-history parameters (time until eclosion and weight upon eclosion) of single Drosophila melanogaster flies. The experimental set up was repeated 5 times between March 2020 and November 2021 at the laboratory facilities of University of Bremen, Germany. Maternal microbiota are crucial for Drosophila melanogaster larval development in ephemeral breeding substrates. However, microbiota are known to vary in composition based on the nutritional substrate of the maternal flies (Riedel & Rohlfs, in preparation). Here we collected data on life-history traits of flies developing in different plant substrate and fly-microbiota combinations, to assess fitness outcomes under autochthonous (microbiota collected from a microcosm with same plant substrate) and allochthonous (microbiota collected from microcosm with different plant substrate) developmental conditions. Microbiota was collected as fecal material of 30 gravid female flies in 15 mL screw-cap tubes overnight and subsequently washed into a suspension with 5 mL of sterile Phosphate buffered saline. In a 2 mL collection tube, 1 mL of plant substrate (apple, banana, cucumber, lemon and tomato) was inoculated with 50 µL of fly fecal microbiota suspension; or 1 mL of artificial lab diet was used without microbiota inoculation. Single axenic first instar larvae, collected as eggs from an outbred Drosophila melanogaster population (Wölfle et al., 2009) were added gently, using a sterile brush. Larvae that succumbed due to the procedure were excluded. Freshly eclosed flies were dried over silica gel and weighed on a precision scale (SE2 ultra-micro balance, Sartorius AG). In the first run of the assay development time and weight of individual flies could not be linked and are thus presented separately with the respective column "Weight" or "Days_to_adult" empty. To further emphasize this missing link "Identifier" is only given with Treatment number for the first set of the overlapping samples that include development time information. Information on microbiota community is included in Riedel and Rohlfs (in preparation), Riedel et al. (preprint, https://doi.org/10.1101/2025.10.14.682149) and raw sequences may be accessed at European Nucleotide Archive (ENA) at EMBL-EBI under accession number PRJEB96953 (fungal) and PRJEB96936 (bacterial).
Supplement to:
Riedel, Elisabeth K; Lender, Laura; Kowallik, Vienna; Pollierer, Melanie M; Rohlfs, Marko (preprint): Ecological context shapes microbial contributions to nutrition and development in Drosophila melanogaster. bioRxiv, https://doi.org/10.1101/2025.10.14.682149
Related to:
Riedel, Elisabeth K; Pollierer, Melanie M: Relative contribution of bacterial, fungal or plant essential amino acid to Drosophila melanogaster tissue-determined from Bayesian mixing model [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.990388
Riedel, Elisabeth K; Pollierer, Melanie M: δ¹³C of amino acids extracted from Drosophila melanogaster and its ontogenetic environments after fly development under different symbiont-environment conditions [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.989943
Riedel, Elisabeth K; Rohlfs, Marko (2025): Amplicon sequencing of 16S V3-V4 region of (1) fecal material of Drosophila melanogaster raised in fruit and vegetable-based microcosms perpetuating field collected microbes (n=26) (2) Pooled ontogenetic environment samples of 10 vials harboring a single fly until eclosion for three different substrates (n=9) (3) Samples of fecal material from flies on laboratory diet and flies transferred to fruit from laboratory diet, as well as blanks are included for comparison [dataset]. European Nucleotide Archive (ENA), insdc:PRJEB96936
Riedel, Elisabeth K; Rohlfs, Marko (2025): Amplicon sequencing of ITS2 region of (1) fecal material of Drosophila melanogaster raised in fruit and vegetable-based microcosms perpetuating field collected microbes (n=26) (2) Pooled ontogenetic environment samples of 10 vials harboring a single fly until eclosion for three different substrates (n=9) (3) Samples of fecal material from flies on laboratory diet and flies transferred to fruit from laboratory diet, as well as blanks are included for comparison [dataset]. European Nucleotide Archive (ENA), insdc:PRJEB96953
References:
Wölfle, Susanne; Trienens, Monika; Rohlfs, Marko (2009): Experimental evolution of resistance against a competing fungus in Drosophila melanogaster. Oecologia, 161(4), 781-790, https://doi.org/10.1007/s00442-009-1414-x
Comment:
Funded by the State of Bremen
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Riedel, Elisabeth K | Microcosm experiment | ||
| 2 | Date/time start, experiment | Date/time start exp | Riedel, Elisabeth K | Larval transfer to exterimental units | Date of larval transfer into experimental units | |
| 3 | Date/time end, experiment | Date/time end exp | Riedel, Elisabeth K | Experimental units were monitored for two more days before they were discarded and demise as larva was assumed. | Date of first day of no fly eclosion | |
| 4 | Sample code/label 2 | Sample label 2 | Riedel, Elisabeth K | "Sample number": Individual number for each sample within one "Run" (Experimental run ID), if known. | ||
| 5 | Substrate type | Substrate | Riedel, Elisabeth K | First dietary and developmental substrate available to flies in microcosm | "Substrate": Organically grown fruit, vegetable (= apple, banana, cucumber, lemon or tomato) purchased at local organic supermarket (ALECO, Bremen), or artificial diet type that was used as substrate in ontogenetic environments (= lab). | |
| 6 | Substrate preparation | Substrate prep | Riedel, Elisabeth K | "Substrate prepartion": Description of the pre-processing of "Substrate" (Substrate type). | ||
| 7 | Treatment | Treat | Riedel, Elisabeth K | "Treatment": Coding for "Substrate" (Substrate type) with the first letter followed by the number of "Fecal_type_numeric" (Fecal type). | ||
| 8 | Fecal type | Fec type | Riedel, Elisabeth K | "Fecal_type_numeric": 1 = apple, 2 = banana, 3 = tomato, and 0 = no inoculation of dietary and developmental substrate available to flies in microcosms predating inoculation of fecal microcbiota on "Substrate" (Substrate type). | ||
| 9 | Treatment number | Treat no | Riedel, Elisabeth K | "Treatment number": Individual number for each sample within one treatment of a "Run" (Experimental run ID), if known. | ||
| 10 | Experimental run ID | Exp run ID | Riedel, Elisabeth K | "Run": Describing whether the experiment was used to obtain the fitness data in Riedel et al., preprint, https://doi.org/10.1101/2025.10.14.682149). | ||
| 11 | Sample number | Sample no | Riedel, Elisabeth K | "Identifier": Unique identifier including F (fly sample) to separate fly and substrate samples taken from the pool for δ¹³C measurement of amino acids, "Treatment", "Treatment number" (Treatment number ID; if applicable, in the first "Run" (Experimental run ID) weight cannot directly be linked to development time of individual fly) and "Run" (Experimental run ID); all are separated by "-". | ||
| 12 | Experiment number ID | Exp no ID | Riedel, Elisabeth K | "Experiment": Single larvae experiment (SL): number of experiments since first experiment. | ||
| 13 | Treatment code | Treat code | Riedel, Elisabeth K | "Inoculated with": Coding for microcosm and microbe transfers within said microcosm predating inoculation on substrates named in "Substrate" (Substrate type). They are separated by tab, first dietary and developmental substrate available to flies in microcosms, second year of collection of said microcosm from Biogarten facilities at University of Bremen, third generation (G) of transfer events, the number of transfers to fresh substrate the microcosm has undergone (about monthly after initial microcosm set-up). | ||
| 14 | Treatment: inoculation substrate | T:inoc substrate | Riedel, Elisabeth K | "SymbiontInoculum_condition": Identifying whether dietary and developmental substrate available to flies in microcosms is identical and hence autochthonous to substrate used in fitness assay (= autochSI), or substrate available to flies in microcosms and substrate used in fitness assay are different and hence allochthonous (= allochSI). | ||
| 15 | Kingdom | Kingdom | Riedel, Elisabeth K | |||
| 16 | Species, unique identification | Species UID | Riedel, Elisabeth K | Reference in ITIS (ITIS) | ||
| 17 | Species, unique identification (URI) | Species UID (URI) | Riedel, Elisabeth K | Reference in ITIS (ITIS) | ||
| 18 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Riedel, Elisabeth K | Reference in ITIS (ITIS) | ||
| 19 | Sex | Sex | Riedel, Elisabeth K | Sexed visually | Sex of adult flies determined after eclosion. | |
| 20 | Drosophila melanogaster, mass per individual | D. melanogaster m/ind | mg/# | Riedel, Elisabeth K | Weighed | "Weight" opf fly |
| 21 | Days to puparium | Days to puparium | days | Riedel, Elisabeth K | Counted from start of experiment until discovery of first puparium | "Days_to_puparium": Number of days passed from the start of experiment until a puparium could be spotted in the experimental unit (2 mL collection tube containing substrate and microbiota inoculum). |
| 22 | Pupal development, duration | Pupal dev dur | days | Riedel, Elisabeth K | Calculated duration between discovery of first puparium and adult fly eclosion from puparium | "Days_pupal_developemt": Number of days between Days_to_puparium and Days_to_adult. |
| 23 | Days to adult | Days to adult | days | Riedel, Elisabeth K | Counted from start of experiment until eclosion from puparium | "Days_to_adult": Number of days passed from the start of experiment until an adult fly eclosed from puparium. |
| 24 | Drosophila melanogaster, survival indicator | D. melanogaster survival ind | Riedel, Elisabeth K | "Survival": 1 = fly eclosion event, 0 = demise as larva. Data collection was stopped earliest after 3 days of no flies eclosing. |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
46684 data points
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