Mayers, Kyle M J; Vollmar, Nele Manon; Cordier, Tristan; Weiner, Agnes K M; Müller, Juliane; De Schepper, Stijn (2026): Polarella glacialis gene copies from Yermak Plateau sediment core KH21-234-34GC1 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.989275
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Published: 2026-01-30 • DOI registered: 2026-01-30
Abstract:
Gene copies of the sympagic dinoflagellate Polarella glacialis were determined by droplet digital PCR on samples from marine sediment core KH21-234-34GC collected from the Yermak Plateu (81.2199280 ˚N, 2.3710955˚E ) with a gravity corer at 1011m water depth during cruise KH21-234. DNA from gravity core sediments was extracted in the NORCE ancient DNA laboratory using the DNeasy PowerMax Soil Kit (Qiagen). Primers targeting the ITS1 region of P. glacialis were used in ddPCR reactions to determine gene copies per g of sediment.
Supplement to:
Mayers, Kyle M J; Vollmar, Nele Manon; Cordier, Tristan; Weiner, Agnes K M; Müller, Juliane; Larsen, Aud; De Schepper, Stijn (2026): First-year sea ice history in the Arctic Ocean inferred from ancient DNA of Polerella glacialis over the past 50,000 years. Earth and Planetary Science Letters, 678, 119809, https://doi.org/10.1016/j.epsl.2025.119809
Funding:
European Research Council (ERC), grant/award no. 101118519: Into the Blue - Resolving past Arctic greenhouse climate (i2B)
Coverage:
Latitude: 81.219928 * Longitude: 2.371096
Date/Time Start: 2021-07-07T14:42:29 * Date/Time End: 2021-07-07T14:42:29
Minimum DEPTH, sediment/rock: 0.10 m * Maximum DEPTH, sediment/rock: 4.50 m
Event(s):
KH21-234-34GC * Latitude: 81.219928 * Longitude: 2.371096 * Date/Time: 2021-07-07T14:42:29 * Elevation: -1011.0 m * Recovery: 466 cm * Campaign: KH21-234 * Basis: Kronprins Haakon * Method/Device: Gravity corer (GC)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Cruise/expedition | Expedition | Mayers, Kyle M J | |||
| 2 | Station label | Station | Mayers, Kyle M J | |||
| 3 | Core | Core | Mayers, Kyle M J | |||
| 4 | Section | Section | # | Mayers, Kyle M J | ||
| 5 | DEPTH, sediment/rock | Depth sed | m | Mayers, Kyle M J | Geocode – Cumulative depth | |
| 6 | Depth, section | Depth sect | cm | Mayers, Kyle M J | Top depth (in section) | |
| 7 | Depth, section | Depth sect | cm | Mayers, Kyle M J | Bottom depth (in section) | |
| 8 | Sample ID | Sample ID | Mayers, Kyle M J | |||
| 9 | Laboratory code/label | Lab label | Mayers, Kyle M J | |||
| 10 | AGE | Age | ka BP | Mayers, Kyle M J | Geocode | |
| 11 | Gene copies per gram sediment | GCN per sed | #/g | Mayers, Kyle M J | Droplet digital polymerase chain reaction (ddPCR); DNA extracted using DNeasy PowerMax Soil Kit (Qiagen) | Polarella glacialis, replicate A |
| 12 | Gene copies per gram sediment | GCN per sed | #/g | Mayers, Kyle M J | Droplet digital polymerase chain reaction (ddPCR); DNA extracted using DNeasy PowerMax Soil Kit (Qiagen) | Polarella glacialis, replicate B |
| 13 | Gene copies per gram sediment | GCN per sed | #/g | Mayers, Kyle M J | Droplet digital polymerase chain reaction (ddPCR); DNA extracted using DNeasy PowerMax Soil Kit (Qiagen) | Polarella glacialis, replicate C |
| 14 | Gene copies per gram sediment | GCN per sed | #/g | Mayers, Kyle M J | Average | Polarella glacialis, Average all replicates |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
816 data points