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Pastor, Ada; Holmboe, Cecilie Marie Hartvig; Han, Xingguo; Frossard, Aline; Romaní, Anna M; Kjær, Jakob B; Tank, Jennifer; Giménez-Grau, Pau; Hille, Erika; D'Acqui, Luigi P; Catalán, Núria; Riis, Tenna (2026): Microbial Nitrogen Cycling Genes in Arctic Streams [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.988290

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Published: 2026-02-09 • DOI registered: 2026-02-09

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Abstract:
This study integrates molecular, environmental, and spatiotemporal data to examine the structure and function of streambed microbial communities across Arctic regions experiencing rapid climate change. Sediment samples were collected from 28 streams spanning four Arctic regions during summer 2021, including 15 Greenlandic streams that were sampled three times throughout the open-water season to capture temporal variation. Microbial community composition was characterized using 16S rRNA gene sequencing, while functional potential for nitrogen cycling was quantified through qPCR targeting six key genes involved in N-fixation (nifH), nitrification (amoA, nxrB), and denitrification (nirS, norB, nosZ). Environmental data included catchment characteristics, streamwater and sediment caractheristics. These molecular and environmental datasets were integrated with spatial and temporal sampling metadata to assess biogeographic and seasonal trends. Together, these data provide a comprehensive framework for understanding how Arctic stream microbial communities and nitrogen cycling functions respond to spatial heterogeneity and seasonal dynamics under ongoing climate change.
Keyword(s):
Arctic; diversity; nitrogen genes; Sediment; stream; water chemistry; Watershed
Supplement to:
Holmboe, Cecilie Marie Hartvig; Riis, Tenna; Han, Xingguo; Frossard, Aline; Romaní, Anna M; Kjær, Jakob B; Tank, Jennifer; Giménez-Grau, Pau; Hille, Erika; D'Acqui, Luigi P; Catalán, Núria; Pastor, Ada (accepted): Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams. Global Biogeochemical Cycles
Related to:
Holmboe, Cecilie Marie Hartvig; Pastor, Ada; Riis, Tenna (2024): Stream Nitrogen Concentrations Across Arctic Vegetation Gradients. Global Biogeochemical Cycles, 38(2), e2023GB007840, https://doi.org/10.1029/2023GB007840
Funding:
Aarhus University (AU), grant/award no. AUFF-E-2020-9-16: Deciphering the role of rocks as nitrogen source for biological cycling in Arctic freshwaters (RockNrole)
Danish National Research Foundation (DG), grant/award no. DNRF185: Center for Ice-Free Arctic Research (CIFAR)
Horizon 2020 (H2020), grant/award no. 730938: Nitrogen sources for Arctic freshwaters (NitroFresh)
Horizon 2020 (H2020), grant/award no. 839709: Linking Chemical diversity and Reactivity of Arctic dissolved Organic Matter for its integration in Earth system model
Spanish Ministry of Science, Innovation and Universities (MICINN), grant/award no. RYC2022-036661-I
Villum Fonden, grant/award no. 00028351: Nitrogen in rocks: an overlooked nutrient source in Arctic aquatic ecosystems
Coverage:
Median Latitude: 73.527082 * Median Longitude: -49.890874 * South-bound Latitude: 67.753333 * West-bound Longitude: -149.592708 * North-bound Latitude: 78.936310 * East-bound Longitude: 12.007480
Date/Time Start: 2021-07-09T00:00:00 * Date/Time End: 2021-09-17T00:00:00
Minimum Elevation: 5.7 m * Maximum Elevation: 890.4 m
Event(s):
Aucella_microbes * Latitude: 74.503270 * Longitude: -20.541790 * Date/Time Start: 2021-07-18T00:00:00 * Date/Time End: 2021-09-12T00:00:00 * Elevation: 153.9 m * Location: Northeast Greenland * Method/Device: Multiple investigations (MULT)
Austre-Bayelva_microbes * Latitude: 78.919940 * Longitude: 11.826250 * Date/Time: 2021-08-23T00:00:00 * Elevation: 52.5 m * Location: Svalbard * Method/Device: Multiple investigations (MULT)
Bayelva_microbes * Latitude: 78.920320 * Longitude: 11.795890 * Date/Time: 2021-08-27T00:00:00 * Elevation: 50.4 m * Location: Svalbard * Method/Device: Multiple investigations (MULT)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventPastor, Ada
2Sample code/labelSample labelPastor, Ada
3Latitude of eventLatitudePastor, Ada
4Longitude of eventLongitudePastor, Ada
5DATE/TIMEDate/TimePastor, AdaGeocode
6Elevation of eventElevationmPastor, Ada
7pHpHPastor, Adastreamwater
8Temperature, waterTemp°CPastor, Adastreamwater
9Conductivity, electricalECµS/cmPastor, Adastreamwater
10Ammonium[NH4]+mg/lPastor, Adastreamwater
11Nitrate[NO3]-mg/lPastor, Adastreamwater
12Carbon, organic, dissolvedDOCmg/lPastor, Adastreamwater
13Nitrogen, organic, dissolvedDONmg/lPastor, Adastreamwater
14Nitrogen, total dissolvedTDNmg/lPastor, Adastreamwater
15pHpHPastor, Adasediment
16Conductivity, electricalECµS/cmPastor, Adasediment
17Size fraction > 2 mm, gravel>2 mm%Pastor, AdaSediment size >2000 m
18Size fraction 2.000-0.630 mm, coarse sand2000-630 µm%Pastor, AdaSediment size 2000-630 m
19Size fraction 0.630-0.200 mm, medium sand630-200 µm%Pastor, AdaSediment size 630-200 m
20Size fraction 0.200-0.063 mm, fine sand200-63 µm fS%Pastor, AdaSediment size 200 63 m
21Loss on ignitionLOI%Pastor, Ada
22Ammonium[NH4]+mg/lPastor, Adasediment
23Nitrate[NO3]-mg/lPastor, Adasediment
24NitrogenN%Pastor, Adasediment
25CarbonC%Pastor, Adasediment
26Normalized Difference Vegetation IndexNDVIPastor, Ada
27Catchment areaCatch areakm2Pastor, Ada
28SlopeSlopedegPastor, Ada
2916S rRNA gene, copy number, per unit sediment dry mass16S rRNA gene/sed dm#/gPastor, Ada
30AOA-amoA gene, copy number, per unit sediment dry massAOA-amoA/sed dm#/gPastor, AdaArchaeal amoA gene (encoding ammonium monooxygenase) in sediment samples, quantified from extracted DNA
31AOB-amoA gene, copy number, per unit sediment dry massAOB-amoA/sed dm#/gPastor, AdaBacterial amoA gene (encoding ammonium monooxygenase) in sediment samples, quantified from extracted DNA
32nifH gene, copy number, per unit sediment dry massnifH/sed dm#/gPastor, AdanifH gene (encoding the nitrogenase enzyme) in sediment samples, quantified from extracted DNA
33nrxB gene, copy number, per unit sediment dry massnrxB/sed dm#/gPastor, AdanrxB gene (encoding the beta subunit of nitrite oxidoreductase) in sediment samples, quantified from extracted DNA
34nosZ gene, copy number, per unit sediment dry massnosZ/sed dm#/gPastor, AdanosZ gene (encoding nitrous oxide reductase) in sediment samples, quantified from extracted DNA
35nirS gene, copy number, per unit sediment dry massnirS/sed dm#/gPastor, AdanirS gene (encoding cytochrome cd1 nitrite reductase) in sediment samples, quantified from extracted DNA
36qnorB gene, copy number, per unit sediment dry massqnorB/sed dm#/gPastor, AdaqnorB gene (encoding quinol-dependent nitric oxide reductase ) in sediment samples, quantified from extracted DNA
License:
Creative Commons Attribution-ShareAlike 4.0 International (CC-BY-SA-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1477 data points

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