Leu, Eva; Šupraha, Luka; Graeve, Martin; Fahl, Kirsten; Müller, Anja; Müller, Juliane; Lunden, Jonas; Woll, Matthias; Kohlbach, Doreen: Relative proportions of fatty acids in Melosira assemblages, and subsequently isolated unialgal cultures collected in June 2023 during the RV Kronprins Haakon cruise 2023-1 in Fram Strait [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.987819 (dataset in review)
Abstract:
During the AO2023-1 expedition (1-21 June 2023), Melosira assemblages were collected using a handpump at a sampling site in Fram Strait. In addition to performing physiological measurements and filtering the material for trophic markers, living material was brought back to Oslo and two strains were established as unialgal cultures. The cultures are maintained in artificial seawater-based L1 medium at 4°C and 25 µmol m-2 s-1 light with a 16:8 L:D cycle. Lipids were extracted from freeze-dried samples following the method of Folch et al. (1957), using a dichloromethane/methanol mixture (2:1, v/v) in a sonication bath. The extracted lipids were converted to fatty acid methyl esters and analyzed by gas chromatography (Agilent 6890N Network GC, Agilent Technologies) equipped with a DB-FFAP capillary column (30 m length, 0.25 mm internal diameter, 0.25 μm film thickness), split injection, and a flame ionization detector. The temperature program ranged from 160°C to 240°C. Fatty acid analysis was conducted to complement datasets on fatty acid stable isotope compositions and sterol proportions. Collectively, the datasets enable a detailed characterization of the biochemical composition of different Melosira arctica-dominated algal assemblages from the Arctic Ocean, and a comparison with a unialgal culture of this important species.
Related to:
Kohlbach, Doreen; Kunisch, Erin H; Graeve, Martin; Fahl, Kirsten; Müller, Anja; Müller, Juliane; Lunden, Jonas; Woll, Matthias; Leu, Eva (submitted): Melosira arctica: algal superfood or nutritional dead-end for Arctic grazers? Elementa - Science of the Anthropocene
References:
Folch, Jordi; Lees, M; Sloane Stanley, G H (1957): A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry, 226(1), 497-509, https://doi.org/10.1016/S0021-9258(18)64849-5
Funding:
Horizon Europe (HorizonEU), grant/award no. 101136875: Ocean and coastal waters carbon- and biodiversity-rich ecosystems and habitats in Europe and the Polar Regions - HORIZON-CL6-2023-CLIMATE-01-3
Coverage:
Latitude: 78.939700 * Longitude: -2.138700
Date/Time Start: 2023-06-18T00:00:00 * Date/Time End: 2023-06-18T00:00:00
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 0 m
Event(s):
AO2023-1_MA1 * Latitude: 78.939700 * Longitude: -2.138700 * Date/Time: 2023-06-18T00:00:00 * Location: Fram Strait * Campaign: AO2023-1 (2024007009) * Basis: Kronprins Haakon * Method/Device: Hand pump (HP)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Leu, Eva | |||
| 2 | Station label | Station | Leu, Eva | |||
| 3 | Type of study | Study type | Leu, Eva | |||
| 4 | Sample code/label | Sample label | Leu, Eva | |||
| 5 | DEPTH, water | Depth water | m | Leu, Eva | Geocode | |
| 6 | Depth comment | Depth comm | Leu, Eva | |||
| 7 | Genus, unique identification | Genus UID | Leu, Eva | Reference in WoRMS (WoRMS) | ||
| 8 | Genus, unique identification (Semantic URI) | Genus UID (Semantic URI) | Leu, Eva | Reference in WoRMS (WoRMS) | ||
| 9 | Genus, unique identification (URI) | Genus UID (URI) | Leu, Eva | Reference in WoRMS (WoRMS) | ||
| 10 | Decanoic acid of total fatty acids | 10:0 | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 11 | Tetradecanoic acid of total fatty acids | 14:0 | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 12 | (E,7R,11R)-3,7,11,15-tetramethylhexadec-2-en-1-ol | Phytol | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 13 | 9-Tetradecenoic acid of total fatty acids | 14:1(n-5) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 14 | iso-Pentadecanoic acid of total fatty acids | i-15:0 | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 15 | Pentadecanoic acid of total fatty acids | 15:0 | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 16 | Hexadecanoic acid of total fatty acids | 16:0 | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 17 | 9-Hexadecenoic acid of total fatty acids | 16:1(n-7) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 18 | 11-Hexadecenoic acid of total fatty acids | 16:1(n-5) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 19 | 9,12-Hexadecadienoic acid of total fatty acids | 16:2(n-4) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 20 | Heptadecanoic acid of total fatty acids | 17:0 | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 21 | 6,9,12-Hexadecatrienoic acid of total fatty acids | 16:3(n-4) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 22 | 6,9,12,15-Hexadecatetraenoic acid of total fatty acids | 16:4(n-1) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 23 | Octadecanoic acid of total fatty acids | 18:0 | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 24 | 9-Octadecenoic acid of total fatty acids | 18:1(n-9) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 25 | 11-Octadecenoic acid of total fatty acids | 18:1(n-7) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 26 | 13-Octadecenoic acid of total fatty acids | 18:1(n-5) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 27 | 9,12-Octadecadienoic acid of total fatty acids | 18:2(n-6) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 28 | 6,9,12,15-Octadecatetraenoic acid of total fatty acids | 18:4(n-3) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 29 | Icosanoic acid of total fatty acids | 20:0 | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 30 | 11-Icosenoic acid of total fatty acids | 20:1(n-9) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 31 | 13-Icosenoic acid of total fatty acids | 20:1(n-7) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 32 | 5,8,11,14-Eicosatetraenoic acid of total fatty acids | 20:4(n-6) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 33 | 11,14,17-Eicosatrienoic acid of total fatty acids | 20:3(n-3) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 34 | 8,11,14,17-Eicosatetraenoic acid of total fatty acids | 20:4(n-3) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 35 | 5,8,11,14,17-Eicosapentaenoic acidof total fatty acids | 20:5(n-3) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 36 | Docosanoic acid of total fatty acids | 22:0 | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 37 | 13-Docosenoic acid of total fatty acids | 22:1(n-9) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 38 | 17-Docosenoic acid of total fatty acids | 22:1(n-5) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 39 | 13,16-Docosadienoic acid of total fatty acids | 22:2(n-6) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 40 | 7,10,13,16-Docosatetraenoic acid of total fatty acids | 22:4(n-6) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 41 | Docosatetraenoic acid 22:4(n-3) of total fatty acids | 22:4(n-3) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 42 | 7,10,13,16,19-Docosapentaenoic acid of total fatty acids | 22:5(n-3) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 43 | Tetracosenoic acid 24:1(n-11) of total fatty acids | 24:1(n-11) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 44 | 15-Tetracosenoic acid of total fatty acids | 24:1(n-9) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 45 | 4,7,10,13,16,19-Docosahexaenoic acid of total fatty acids | 22:6(n-3) | % | Leu, Eva | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 46 | Date/time start, experiment | Date/time start exp | Leu, Eva | Unialgal cultures cultivation | ||
| 47 | Date/time end, experiment | Date/time end exp | Leu, Eva | Unialgal cultures cultivation | ||
| 48 | Experimental treatment | Exp treat | Leu, Eva | Unialgal cultures cultivation | ||
| 49 | Treatment: temperature | T:temp | °C | Leu, Eva | Unialgal cultures cultivation | |
| 50 | Treatment: salinity | T:sal | Leu, Eva | Unialgal cultures cultivation | ||
| 51 | Treatment: nitrate | T:[NO3]- | µmol/l | Leu, Eva | Unialgal cultures cultivation | as NaNO3 |
| 52 | Treatment: silicate | T:[Si(OH)4] | µmol/l | Leu, Eva | Unialgal cultures cultivation | as Na2SiO3 |
| 53 | Treatment: phosphate | T:[PO4]3- | µmol/l | Leu, Eva | Unialgal cultures cultivation | as K2HPO4 |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
555 data points