Kohlbach, Doreen; Kunisch, Erin H; Graeve, Martin; Fahl, Kirsten; Müller, Anja; Müller, Juliane; Lunden, Jonas; Woll, Matthias; Leu, Eva: Relative proportions of fatty acids in Melosira assemblages, pelagic and ice-associated particulate organic matter (PPOM and IPOM) collected during the RV Kronprins Haakon Arctic Ocean cruise 2024 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.987058 (dataset in review)
Abstract:
During the AO2024 expedition (19 July to 12 August 2024), Melosira assemblages were collected using a handheld net at two sampling sites in the Amundsen Basin. Pelagic particulate organic matter (PPOM) was sampled at 13 stations spanning the Amundsen and Nansen Basins as well as the Gakkel Ridge, using Niskin bottles mounted on a CTD rosette. Between 3 and 6.5 L of seawater were filtered through GF/F filters. Ice-associated POM was obtained using a Kovacs 9 cm ice corer at one station each in the Amundsen and Nansen Basins; the bottom 10 cm of each ice core were melted with the addition of filtered seawater and filtered onto GF/F filters.
Lipids were extracted from freeze-dried samples following the method of Folch et al. (1957), using a dichloromethane/methanol mixture (2:1, v/v) in a sonication bath. The extracted lipids were converted to fatty acid methyl esters and analyzed by gas chromatography (Agilent 6890N Network GC, Agilent Technologies) equipped with a DB-FFAP capillary column (30 m length, 0.25 mm inner diameter, 0.25 μm film thickness), split injection, and a flame ionization detector. The temperature program ranged from 160°C to 240°C. Fatty acids were identified via standard mixtures and quantified using an internal standard (C23:0).
Fatty acid analysis was conducted to complement datasets on fatty acid stable isotope compositions and sterol proportions. Collectively, the datasets enable a detailed characterization of the biochemical composition of different algal assemblages across the central Arctic Ocean.
Supplement to:
Kohlbach, Doreen; Kunisch, Erin H; Graeve, Martin; Fahl, Kirsten; Müller, Anja; Müller, Juliane; Lunden, Jonas; Woll, Matthias; Leu, Eva (submitted): Melosira arctica: algal superfood or nutritional dead-end for Arctic grazers? Elementa - Science of the Anthropocene
Related to:
Dodd, Paul Anthony; Wold, Anette; Ahmed, Fowzia; Darecki, Miroslaw; Duncan, Rebecca J; Fossan, Kristen; Goraguer, Lucie; Granskog, Mats A; Itkin, Polona; Kern, Yannick; Koenig, Zoé; Kohlbach, Doreen; Kovacs, Kit Maureen; Kowalczuk, Piotr; Christensen, Jakob; Leu, Eva; Lydersen, Christian; Muilwijk, Morven; Nowak, Benia; Randelhoff, Achim; Stürzinger, Vegard; Søreide, Janne E; Widera, Sara; Sviggum Helgerud, Trine Lise (2024): Arctic Ocean 2024 - Cruise Report. Norwegian Polar Institute, Tromsø, Norway, AO2024_cruise_report.pdf
References:
Folch, Jordi; Lees, M; Sloane Stanley, G H (1957): A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry, 226(1), 497-509, https://doi.org/10.1016/S0021-9258(18)64849-5
Project(s):
Funding:
Helmholtz Association of German Research Centres (HGF), grant/award no. VH-NG-20-09: Resilience and vulnerability of the central Arctic Ocean food web to cumulative stress by warming and anthropogenic pollution (Double-Trouble)
Horizon Europe (HorizonEU), grant/award no. 101136875: Ocean and coastal waters carbon- and biodiversity-rich ecosystems and habitats in Europe and the Polar Regions - HORIZON-CL6-2023-CLIMATE-01-3
Coverage:
Median Latitude: 85.386094 * Median Longitude: 1.560512 * South-bound Latitude: 81.823100 * West-bound Longitude: -34.800100 * North-bound Latitude: 87.794600 * East-bound Longitude: 30.169800
Date/Time Start: 2024-07-23T11:05:54 * Date/Time End: 2024-08-10T05:14:44
Minimum DEPTH, water: 15 m * Maximum DEPTH, water: 45 m
Event(s):
AO2024_IPOM_17 * Latitude: 83.941000 * Longitude: 22.125200 * Date/Time: 2024-08-06T12:40:09 * Location: Central Arctic Ocean * Campaign: AO2024 (2024007009) * Basis: Kronprins Haakon * Method/Device: Ice drilling corer (Kovacs) * Comment: Kovacs 9 cm ice corer
AO2024_IPOM_37 * Latitude: 86.576100 * Longitude: -4.877900 * Date/Time: 2024-07-31T06:21:20 * Elevation: -4232.0 m * Location: Central Arctic Ocean * Campaign: AO2024 (2024007009) * Basis: Kronprins Haakon * Method/Device: Ice drilling corer (Kovacs) * Comment: Kovacs 9 cm ice corer
AO2024_MA1 * Latitude: 86.719700 * Longitude: -34.121300 * Date/Time: 2024-07-23T11:05:54 * Elevation: -3608.0 m * Location: Central Arctic Ocean * Campaign: AO2024 (2024007009) * Basis: Kronprins Haakon * Method/Device: Hand net (HN)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Kohlbach, Doreen | |||
| 2 | Latitude of event | Latitude | Kohlbach, Doreen | |||
| 3 | Longitude of event | Longitude | Kohlbach, Doreen | |||
| 4 | Elevation of event | Elevation | m | Kohlbach, Doreen | ||
| 5 | Station label | Station | Kohlbach, Doreen | |||
| 6 | Device type | Device | Kohlbach, Doreen | |||
| 7 | Sample code/label | Sample label | Kohlbach, Doreen | |||
| 8 | DATE/TIME | Date/Time | Kohlbach, Doreen | Geocode | ||
| 9 | DEPTH, water | Depth water | m | Kohlbach, Doreen | Geocode | |
| 10 | Sample comment | Sample comment | Kohlbach, Doreen | |||
| 11 | Genus, unique identification | Genus UID | Kohlbach, Doreen | Reference in WoRMS (WoRMS) | ||
| 12 | Genus, unique identification (Semantic URI) | Genus UID (Semantic URI) | Kohlbach, Doreen | Reference in WoRMS (WoRMS) | ||
| 13 | Genus, unique identification (URI) | Genus UID (URI) | Kohlbach, Doreen | Reference in WoRMS (WoRMS) | ||
| 14 | Decanoic acid of total fatty acids | 10:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 15 | Undecanoic acid of total fatty acids | 11:00 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 16 | Dodecanoic acid of total fatty acids | 12:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 17 | Tridecanoid acid of total fatty acids | 13:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 18 | Tetradecanoic acid of total fatty acids | 14:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 19 | 9-Tetradecenoic acid of total fatty acids | 14:1(n-5) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 20 | iso-Pentadecanoic acid of total fatty acids | i-15:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 21 | 12-methyl-Tetradecanoic acid of total fatty acids | ai-15:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 22 | Pentadecanoic acid of total fatty acids | 15:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 23 | 10-Pentadecenoic acid of total fatty acids | 15:1(n-5) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 24 | Hexadecanoic acid of total fatty acids | 16:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 25 | 9-Hexadecenoic acid of total fatty acids | 16:1(n-7) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 26 | 11-Hexadecenoic acid of total fatty acids | 16:1(n-5) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 27 | iso-Heptadecanoic acid of total fatty acids | i-17:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 28 | 9,12-Hexadecadienoic acid of total fatty acids | 16:2(n-4) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 29 | Heptadecanoic acid of total fatty acids | 17:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 30 | 6,9,12-Hexadecatrienoic acid of total fatty acids | 16:3(n-4) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 31 | 6,9,12,15-Hexadecatetraenoic acid of total fatty acids | 16:4(n-1) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 32 | Octadecanoic acid of total fatty acids | 18:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 33 | 9-Octadecenoic acid of total fatty acids | 18:1(n-9) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 34 | 11-Octadecenoic acid of total fatty acids | 18:1(n-7) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 35 | 13-Octadecenoic acid of total fatty acids | 18:1(n-5) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 36 | 9,12-Octadecadienoic acid of total fatty acids | 18:2(n-6) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 37 | 6,9,12-Octadecatrienoic acid of total fatty acids | 18:3(n-6) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 38 | 9,12,15-Octadecatrienoic acid of total fatty acids | 18:3(n-3) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 39 | 6,9,12,15-Octadecatetraenoic acid of total fatty acids | 18:4(n-3) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 40 | Icosanoic acid of total fatty acids | 20:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 41 | 9-Icosenoic acid of total fatty acids | 20:1(n-11) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 42 | 11-Icosenoic acid of total fatty acids | 20:1(n-9) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 43 | 13-Icosenoic acid of total fatty acids | 20:1(n-7) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 44 | 15-Icosenoic acid of total fatty acids | 20:1(n-5) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 45 | 11,14-Eicosadienoic acid of total fatty acids | 20:2(n-6) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 46 | 8,11,14-Eicosatrienoic acid of total fatty acids | 20:3(n-6) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 47 | 5,8,11,14-Eicosatetraenoic acid of total fatty acids | 20:4(n-6) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 48 | 11,14,17-Eicosatrienoic acid of total fatty acids | 20:3(n-3) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 49 | 8,11,14,17-Eicosatetraenoic acid of total fatty acids | 20:4(n-3) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 50 | 5,8,11,14,17-Eicosapentaenoic acidof total fatty acids | 20:5(n-3) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 51 | Docosanoic acid of total fatty acids | 22:0 | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 52 | 11-Docosenoic acid of total fatty acids | 22:1(n-11) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 53 | 13-Docosenoic acid of total fatty acids | 22:1(n-9) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 54 | 13,16-Docosadienoic acid of total fatty acids | 22:2(n-6) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 55 | 7,10,13,16-Docosatetraenoic acid of total fatty acids | 22:4(n-6) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 56 | 7,10,13,16,19-Docosapentaenoic acid of total fatty acids | 22:5(n-3) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 57 | Tetracosenoic acid 24:1(n-11) of total fatty acids | 24:1(n-11) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 58 | 15-Tetracosenoic acid of total fatty acids | 24:1(n-9) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N | |
| 59 | 4,7,10,13,16,19-Docosahexaenoic acid of total fatty acids | 22:6(n-3) | % | Kohlbach, Doreen | Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2116 data points
Download Data (login required; moratorium until 2027-10-27)
View dataset as HTML (shows only first 2000 rows)