Stief, Peter: Degradation of diatom-derived dissolved organic matter (DOM) in surface seawater [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.974879 (dataset in review)
Abstract:
Stored surface seawater originally collected in the North Sea was amended with dissolved organic matter (DOM) obtained from the diatom Skeletonema marinoi by exposing the diatom culture to a hydrostatic pressure level of 40 MPa for 24 h. To this end, S. marinoi had been grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. Diatom-cell-free DOM was added to aliquoted seawater samples at initial concentrations of 250 µmol C/L. The microbial degradation of the added DOM and the microbial response to DOM-amendment at 15°C and in darkness was followed for 2 weeks. The seawater was subsampled at defined time intervals to analyze a number of variables.
Keyword(s):
Supplement to:
Stief, Peter; Niggemann, Jutta; Bligh, Margot; Buck-Wiese, Hagen; Schauberger, Clemens; Wünsch, Urban; Steinke, Michael; Hehemann, Jan-Hendrik; Glud, Ronnie N (in prep.): Pressure-induced leakage of dissolved organic matter from sinking diatom aggregates.
Related to:
Stief, Peter; Schauberger, Clemens; Becker, Kevin W; Elvert, Marcus; Balmonte, John Paul; Franco-Cisterna, Belén; Middelboe, Mathias; Glud, Ronnie N (2023): Hydrostatic pressure induces transformations in the organic matter and microbial community composition of marine snow particles. Communications Earth & Environment, 4(1), 377, https://doi.org/10.1038/s43247-023-01045-4
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Latitude: 54.155000 * Longitude: 7.841000
Date/Time Start: 2019-04-07T12:00:00 * Date/Time End: 2019-04-07T13:00:00
Event(s):
SW_042019 * Latitude: 54.155000 * Longitude: 7.841000 * Date/Time Start: 2019-04-07T12:00:00 * Date/Time End: 2019-04-07T13:00:00 * Method/Device: CTD/Rosette (CTD-RO)
Comment:
Information about the used cultured organisms:
Skeletonema marinoi, Bigelow National Center for Marine Algae and Microbiota, Strain No. CCMP1332, isolated from Long Island Sound (41.2264 N, -73.0639 W) (isolation date is unknown); https://ncma.bigelow.org/CCMP1332
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Stief, Peter | |||
| 2 | Date/time start, experiment | Date/time start exp | Stief, Peter | |||
| 3 | Date/time end, experiment | Date/time end exp | Stief, Peter | |||
| 4 | Species, unique identification | Species UID | Stief, Peter | |||
| 5 | Treatment: carbon | T:C | µmol/l | Stief, Peter | Diatom-derived dissolved organic matter (DOM) was added at the start of the incubation | |
| 6 | Treatment: duration | T:duration | days | Stief, Peter | Incubation time | |
| 7 | Carbon, organic, dissolved | DOC | µmol/l | Stief, Peter | Total organic carbon analyzer, Shimadzu, TOC-L | Concentration of dissolved organic carbon (DOC) in seawater |
| 8 | Nitrogen, total dissolved | TDN | µmol/l | Stief, Peter | Total organic carbon analyzer, Shimadzu, TOC-L | Concentration of total dissolved nitrogen (TDN) in seawater |
| 9 | Proteins, total | TPRT | mg/l | Stief, Peter | Lowry assay after Lowry et al. 1951 | Concentration of total dissolved proteins in seawater |
| 10 | Polysaccharides | Polysacch | µg/ml | Stief, Peter | PAHBAH reducing sugar assay (Lever 1972) | Concentration of total dissolved polysaccharides in seawater |
| 11 | Bacteria | Bact | #/ml | Stief, Peter | Fluorescence determination of DAPI stain | Automated microscopic cell counts and image analysis using ACMEtool3 (Bennke et al. 2016) |
| 12 | Respiration rate, oxygen | Resp O2 | µmol/l/day | Stief, Peter | Oxygen logger, FireSting, Pyroscience | Optode patches on the inner wall of the glass vials were interrogated from outside using a FireSting Oxygen logger (Pyroscience, Aachen, Germany) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1424 data points