Stief, Peter: Pressure-induced leakage of dissolved organic matter (DOM) from diatom-bacteria aggregates [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.974878 (dataset in review)
Abstract:
Model diatom aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of seawater collected in Oeresund (Stief et al. 2023), Central Arctic Ocean, and Japan Trench. S. marinoi was grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. The diatom aggregates were individually incubated at either atmospheric pressure (0.1 MPa = control treatment) or at gradually increasing hydrostatic pressure (0.1-100 MPa = pressure treatment) throughout 20 days in darkness and at 3°C. The pressure treatment simulated the sinking of diatom-bacteria aggregates from the ocean surface down into a hadal trench of 10 km depth. Pressure-induced leakage of dissolved organic matter (DOM) was followed throughout the incubations. Ambient concentrations of different DOM components were measured: Dissolved organic carbon (DOC), total dissolved nitrogen (TDN), protein-like and humic-like DOM fluorescence.
Keyword(s):
Supplement to:
Stief, Peter; Niggemann, Jutta; Bligh, Margot; Buck-Wiese, Hagen; Schauberger, Clemens; Wünsch, Urban; Steinke, Michael; Hehemann, Jan-Hendrik; Glud, Ronnie N (in prep.): Pressure-induced leakage of dissolved organic matter from sinking diatom aggregates.
Related to:
Stief, Peter; Schauberger, Clemens; Becker, Kevin W; Elvert, Marcus; Balmonte, John Paul; Franco-Cisterna, Belén; Middelboe, Mathias; Glud, Ronnie N (2023): Hydrostatic pressure induces transformations in the organic matter and microbial community composition of marine snow particles. Communications Earth & Environment, 4(1), 377, https://doi.org/10.1038/s43247-023-01045-4
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Median Latitude: 57.551826 * Median Longitude: 47.925020 * South-bound Latitude: 34.012600 * West-bound Longitude: 12.564700 * North-bound Latitude: 85.432729 * East-bound Longitude: 141.860600
Date/Time Start: 2021-07-22T12:00:00 * Date/Time End: 2023-10-11T00:30:00
Minimum Elevation: -5814.0 m * Maximum Elevation: -28.0 m
Event(s):
PS138_218-1 * Latitude: 85.432729 * Longitude: 60.070401 * Date/Time: 2023-09-20T03:01:38 * Elevation: -3881.0 m * O2A Registry URI: registry.o2a-data.de * Campaign: PS138 (ArcWatch-1) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: IceStation #9, at max depth
SW_072021 * Latitude: 56.104600 * Longitude: 12.564700 * Date/Time Start: 2021-07-22T12:00:00 * Date/Time End: 2021-07-22T13:00:00 * Elevation: -28.0 m * Method/Device: CTD/Rosette (CTD-RO)
SW_102021 * Latitude: 56.104600 * Longitude: 12.564700 * Date/Time Start: 2021-10-17T12:00:00 * Date/Time End: 2021-10-17T13:00:00 * Elevation: -28.0 m * Method/Device: CTD/Rosette (CTD-RO)
Comment:
Information about the used cultured organisms:
Skeletonema marinoi, Bigelow National Center for Marine Algae and Microbiota, Strain No. CCMP1332, isolated from Long Island Sound (41.2264 N, -73.0639 W) (isolation date is unknown); https://ncma.bigelow.org/CCMP1332
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Sample code/label | Sample label | Stief, Peter | |||
| 3 | Type of study | Study type | Stief, Peter | |||
| 4 | Date/time start, experiment | Date/time start exp | Stief, Peter | |||
| 5 | Date/time end, experiment | Date/time end exp | Stief, Peter | |||
| 6 | Species, unique identification | Species UID | Stief, Peter | Diatom strains were obtained from culture collections | ||
| 7 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Stief, Peter | Diatom strains were obtained from culture collections | ||
| 8 | Species, unique identification (URI) | Species UID (URI) | Stief, Peter | Diatom strains were obtained from culture collections | ||
| 9 | Treatment: duration | T:duration | days | Stief, Peter | Incubation time | |
| 10 | Treatment: pressure | T:pressure | MPa | Stief, Peter | Pressure level in incubation tanks | |
| 11 | Carbon, organic, dissolved | DOC | µmol/l | Stief, Peter | Total organic carbon analyzer, Shimadzu, TOC-L | Ambient concentration of dissolved organic carbon (DOC) in aggregate incubations |
| 12 | Nitrogen, total dissolved | TDN | µmol/l | Stief, Peter | Total organic carbon analyzer, Shimadzu, TOC-L | Ambient concentration of total dissolved nitrogen (TDN) in aggregate incubations |
| 13 | Fluorescence, protein-like, component | Cp | RU | Stief, Peter | Fluorescence spectrometer, AquaLog, HORIBA JobinYvon | |
| 14 | Fluorescence, humic-like, component | Ch | RU | Stief, Peter | Fluorescence spectrometer, AquaLog, HORIBA JobinYvon |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2420 data points
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