Citation:

PANGAEA.974747 (dataset in review)

Name: Incubation Experiments of DOM with Prokaryotic Community at the NW Mediterranean Sea site MOLA in 2021
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bacteria; DOM; experimental data; Mediterranean Sea

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Abstract:

The water samples for the incubation experiment were collected on the RV Néréïs II at the Microbial Observatory Laboratory Arago 42N and 4E on 31 May and 26 August 2021. The parameters include DOC/TDN/DON/TDP/DOP, dissolved hydrolyzable amino acids, fluorescent dissolved organic matter (FDOM), enzyme activity, prokaryote heterotrophic production (PHP), prokaryote and viral abundance. The incubation temperature was temperature +13ºC for both experiments. DOC/TDN was analyzed using High-Temperature Catalytic Oxidation (TOC-VCSH). DIN/TDP/DOP was analyzed using a Technicon Auto Analyzer. Dissolved hydrolyzable amino acids were analyzed using High Performal Liquid Chromatography (HPLC). FDOM was measured on a FP-8500 Spectrofluorimeter (Jasco, Japan). Enzymatic activities were measured using 4-methylumbelliferyl-butyrate (esterase), 4-methylumbelliferyl phosphate for alkaline phosphatase (APase), and L-leucine-7-amido-4-methyl coumarin for leu-aminopeptidase (AMA). PHP was measured using 3h-leucine and the microcentrifuge method. Prokaryote and viral abundance were measured on a flow cytometer (Cytoflex, Beckman Coulter, USA). The purpose of data collection was to understand the seasonal cycling of organic matter and heterotrophic bacteria dynamics within the microbial loop.

Keyword(s):

Bacteria; DOM; experimental data; Mediterranean Sea

Supplement to:

Ortega-Retuerta, Eva; Bouchachi, Nawal; Crispi, Olivier; Marie, Barbara; Campos, Rebeca; Paulin, Charles-Hubert; Escoubeyrou, Karine; Colombet, Jonathan; Sime-Ngando, Telesphore; von Jackowski, Anabel (submitted): Temporal changes in DOM quality determine prokaryotic activities and extracellular release in the NW Mediterranean Sea: An Experimental Approach. Environmental Microbiology Reports

Project(s):

Monitoring the impact of climate change on hydrology and biogeochemical cycles in the Gulf of Lion (MOLA) (MOOSE-MOLA)

Funding:

Agence Nationale de la Recherche (ANR), grant/award no. ANR-20-CE01-0007 : MicroPUMP

Coverage:

Latitude: 42.454440 * Longitude: 3.690280

Date/Time Start: 2021-05-31T00:00:00 * Date/Time End: 2021-08-26T00:00:00

Event(s):

MOLA_Aug2021 * Latitude: 42.454440 * Longitude: 3.690280 * Date/Time: 2021-08-26T00:00:00 * Location: Mediterranean Sea * Campaign: (MOOSE (MOLA) 2021) * Basis: * Method/Device: (NIS)

MOLA_May2021 * Latitude: 42.454440 * Longitude: 3.690280 * Date/Time: 2021-05-31T00:00:00 * Location: Mediterranean Sea * Campaign: (MOOSE (MOLA) 2021) * Basis: * Method/Device: (NIS)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Sample code/label	Sample label		Ortega-Retuerta, Eva		May/August refer to the time the samples were taken
2	Replicate	Repl		Ortega-Retuerta, Eva
3	Date/time analysis, experiment	Date/time analysis exp		Ortega-Retuerta, Eva
4	Depth, sampling	Depth sampling	m	Ortega-Retuerta, Eva		The water samples were taken at 500-m depth, but they were incubated with DOM from 5-m depth.
5	Carbon, organic, dissolved	DOC	µmol/l	Marie, Barbara	High temperature catalytic oxidation (TOC-VCSH)
6	Nitrogen, total dissolved	TDN	µmol/l	Marie, Barbara	High temperature catalytic oxidation (TOC-VCSH)
7	Nitrogen, inorganic, dissolved	DIN	µmol/l	Crispi, Olivier	Auto-analyzer, Technicon AA-II	Sum of Nitrate and Nitrite
8	Ammonium	[NH4]+	µmol/l	Crispi, Olivier	Auto-analyzer, Technicon AA-II
9	Nitrogen, organic, dissolved	DON	µmol/l	Crispi, Olivier	Calculated
10	Phosphorus, total dissolved	TDP	µmol/l	Crispi, Olivier	Auto-analyzer, Technicon AA-II
11	Phosphorus, inorganic, dissolved	DIP	µmol/l	Crispi, Olivier	Auto-analyzer, Technicon AA-II
12	Phosphorus, organic, dissolved	DOP	µmol/l	Crispi, Olivier	Calculated
13	L-Aspartic acid	L-Asp	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
14	L-Glutamic acid	L-Glu	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
15	L-Serine	L-Ser	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
16	L-Threonine	L-Thr	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
17	L-Arginine	L-Arg	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
18	L-Histidine	L-His	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
19	L-Alanine	L-Ala	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
20	L-Tyrosine	L-Tyr	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
21	L-Valine	L-Val	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
22	L-Phenylalanine	L-Phe	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
23	L-Leucine	L-Leu	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
24	L-Lysine	L-Lys	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
25	beta-Alanine	b-ala	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
26	D-Aspartic acid	D-Asp	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
27	D-Glutamic acid	D-Glu	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
28	D-Serine	D-Ser	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
29	D-Alanine	D-Ala	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
30	D-Leucine	D-Leu	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
31	Glycine	Gly	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
32	gamma-Aminobutyric acid	g-ABA	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
33	Methionine	Met	nmol/l	Escoubeyrou, Karine	High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000
34	Fluorescence, dissolved organic matter, A coble-peak	FDOM A-peak	RU	Ortega-Retuerta, Eva	Excitation Emission Spectrofluorimeter, 260 nm/450 nm, JASCO FP-8500
35	Fluorescence, dissolved organic matter, B coble-peak	FDOM B-peak	RU	Ortega-Retuerta, Eva	Excitation Emission Spectrofluorimeter, 260 nm/450 nm, JASCO FP-8500
36	Fluorescence, dissolved organic matter, C coble-peak	FDOM C-peak	RU	Ortega-Retuerta, Eva	Excitation Emission Spectrofluorimeter, 260 nm/450 nm, JASCO FP-8500
37	Fluorescence, dissolved organic matter, M coble-peak	FDOM M-peak	RU	Ortega-Retuerta, Eva	Excitation Emission Spectrofluorimeter, 260 nm/450 nm, JASCO FP-8500
38	Fluorescence, dissolved organic matter, T coble-peak	FDOM T-peak	RU	Ortega-Retuerta, Eva	Excitation Emission Spectrofluorimeter, 260 nm/450 nm, JASCO FP-8500
39	Alkaline phosphatase activity	ALKP A	nmol/l/h	Ortega-Retuerta, Eva	Excitation Emission Spectrofluorimeter, 260 nm/450 nm, JASCO FP-8500
40	Esterase activity	FDA	nmol/l/h	Ortega-Retuerta, Eva	Excitation Emission Spectrofluorimeter, 260 nm/450 nm, JASCO FP-8500
41	Leucine aminopeptidase activity	Leu aminopep	nmol/l/h	Ortega-Retuerta, Eva	Excitation Emission Spectrofluorimeter, 260 nm/450 nm, JASCO FP-8500
42	Leucine incorporation rate	Leu inc rate	pmol/l/h	Ortega-Retuerta, Eva	3H-leucine microcentrifuge
43	Bacteria	Bact	#/ml	Ortega-Retuerta, Eva	Flow cytometer, Beckman Coulter, CytoFLEX
44	Virus, high DNA fluorescence	Virus HDNA	#/ml	Colombet, Jonathan	FACSCalibur flow-cytometer (Becton Dickinson)
45	Virus, low DNA fluorescence	Virus LDNA	#/ml	Colombet, Jonathan	FACSCalibur flow-cytometer (Becton Dickinson)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1332 data points

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