Adenaya, Adenike; Rios-Quintero, Ruben; Lara-Martín, Pablo A; Zumbika, Florence; Wurl, Oliver; Ribas-Ribas, Mariana; Hamprecht, Axel; Brinkhoff, Thorsten: Antibiotic susceptibility test of bacterial isolates obtained in the presence of high ciprofloxacin concentration [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.974344 (dataset in review), In: Adenaya, A et al.: High concentrations of ciprofloxacin reduced bacterial abundance and enrichment in the sea surface microlayer (SML) and underlying water (ULW) and exerted selective pressure on marine bacteria [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.974345 (dataset in review)
Abstract:
Antibiotic resistance is a significant global health challenge that is prevalent across various environmental settings, including coastal waters. This study conducted antibiotic susceptibility tests on bacterial isolates obtained from the surface marine layer and underlying water, which had been cultured under high concentrations of ciprofloxacin. The isolates were previously grown on both marine agar (MA) and Mueller Hinton agar (MHA), each supplemented with 100 ng mL-1 of ciprofloxacin. The antibiotics evaluated during the susceptibility testing were those detected in environmental samples. Although the concentrations of antibiotics found in these samples were low. We used clinically relevant concentrations for the antibiotic susceptibility test in this research. The assessed antibiotics included novobiocin (NV; 5 μg), ofloxacin (OFL; 5 μg), clarithromycin (CLR; 15 μg), erythromycin (E; 10 μg), clindamycin (DA; 10 μg), and ciprofloxacin (CIP; 5 μg). Antibiotic susceptibility test was carried out on MA and MHA and zones of inhibition of each antibiotic were recorded.
Keyword(s):
Funding:
German Research Foundation (DFG), grant/award no. 451574234: FOR 5267 - Biogeochemical processes and Air-sea exchange in the Sea-Surface microlayer (BASS)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Adenaya, Adenike | |||
| 2 | Type | Type | Adenaya, Adenike | Type of laboeatory experiment | ||
| 3 | Date/time start, experiment | Date/time start exp | Adenaya, Adenike | |||
| 4 | Date/time end, experiment | Date/time end exp | Adenaya, Adenike | |||
| 5 | Sample type | Samp type | Adenaya, Adenike | |||
| 6 | Treatment | Treat | Adenaya, Adenike | |||
| 7 | Analytical method | Method | Adenaya, Adenike | |||
| 8 | Disc diameter | dd | mm | Adenaya, Adenike | ||
| 9 | Species | Species | Adenaya, Adenike | Species, unique identification | ||
| 10 | Inhibition zone | Inh zone | mm | Adenaya, Adenike | See sample method column | Caused by ciprofloxacin (CIP; 5 µg) |
| 11 | Inhibition zone | Inh zone | mm | Adenaya, Adenike | See sample method column | Caused by novobiocin (NV; 5 µg) |
| 12 | Inhibition zone | Inh zone | mm | Adenaya, Adenike | See sample method column | Caused by ofloxacin (OFL; 5 µg) |
| 13 | Inhibition zone | Inh zone | mm | Adenaya, Adenike | See sample method column | Caused by clarithromycin (CLR; 15 µg) |
| 14 | Inhibition zone | Inh zone | mm | Adenaya, Adenike | See sample method column | Caused bx erythromycin (E; 10 µg) |
| 15 | Inhibition zone | Inh zone | mm | Adenaya, Adenike | See sample method column | Caused by clindamycin (DA;10 µg) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1200 data points