Adenaya, Adenike; Rios-Quintero, Ruben; Lara-Martín, Pablo A; Zumbika, Florence; Wurl, Oliver; Ribas-Ribas, Mariana; Hamprecht, Axel; Brinkhoff, Thorsten: Bacterial abundance in different ciprofloxacin concentrations [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.974340 (dataset in review), In: Adenaya, A et al.: High concentrations of ciprofloxacin reduced bacterial abundance and enrichment in the sea surface microlayer (SML) and underlying water (ULW) and exerted selective pressure on marine bacteria [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.974345 (dataset in review)
Abstract:
A knowledge gap exists regarding the ecological effects of antibiotics on bacterial communities in the sea surface microlayer of coastal waters. The effects of varying concentrations of ciprofloxacin on bacterial abundance in the sea surface microlayer and the corresponding underlying water were assessed using flow cytometry. The samples were amended with different concentrations of ciprofloxacin (0, 10, 50, and 100 ng mL⁻¹) and then incubated at 4 °C in the dark. Aliquots of 1.5 mL were taken at various time points and the cell numbers were subsequently measured using an Accuri C6-1 Flow Cytometer (BD Bioscience, USA).
Keyword(s):
References:
Giebel, Helge-Ansgar; Wolterink, Mathias; Brinkhoff, Thorsten; Simon, Meinhard (2019): Complementary energy acquisition via aerobic anoxygenic photosynthesis and carbon monoxide oxidation by Planktomarina temperata of the Roseobacter group. FEMS Microbiology Ecology, 95(5), fiz050, https://doi.org/10.1093/femsec/fiz050
Funding:
German Research Foundation (DFG), grant/award no. 451574234: FOR 5267 - Biogeochemical processes and Air-sea exchange in the Sea-Surface microlayer (BASS)
Coverage:
Date/Time Start: 2023-05-24T08:00:00 * Date/Time End: 2023-05-31T15:00:00
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 1 m
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Adenaya, Adenike | |||
| 2 | Type | Type | Adenaya, Adenike | Type of laboeatory experiment | ||
| 3 | DATE/TIME | Date/Time | Adenaya, Adenike | Geocode | ||
| 4 | Sample type | Samp type | Adenaya, Adenike | |||
| 5 | DEPTH, water | Depth water | m | Adenaya, Adenike | Geocode | |
| 6 | Sample code/label | Sample label | Adenaya, Adenike | |||
| 7 | Sample comment | Sample comment | Adenaya, Adenike | Fixed | ||
| 8 | Treatment: fixative type | T: fixative type | Adenaya, Adenike | |||
| 9 | Treatment: fixative | T: fixative | % | Adenaya, Adenike | ||
| 10 | Treatment: fixative | T: fixative | ml | Adenaya, Adenike | ||
| 11 | Sample volume | Samp vol | ml | Adenaya, Adenike | ||
| 12 | Sample storage | Samp storage | Adenaya, Adenike | |||
| 13 | Temperature, device | T Device | °C | Adenaya, Adenike | Storage Temperature | |
| 14 | Device type | Device | Adenaya, Adenike | |||
| 15 | Reference/source | Reference | Adenaya, Adenike | Protocol | ||
| 16 | Treatment: ciprofloxacin | T: ciprofloxacin | ng/ml | Adenaya, Adenike | See Device type column | |
| 17 | Time point, descriptive | Time point | Adenaya, Adenike | See Device type column | 3 days interval | |
| 18 | Replicates | Repl | # | Adenaya, Adenike | See Device type column | |
| 19 | Counts | Counts | # | Adenaya, Adenike | See Device type column | Bead count |
| 20 | Volume | V | µl | Adenaya, Adenike | See Device type column | |
| 21 | Bacteria | Bact | #/ml | Adenaya, Adenike | See Device type column | |
| 22 | Dilution factor | Dilution | Adenaya, Adenike | See Device type column | ||
| 23 | Bacteria | Bact | #/ml | Adenaya, Adenike | See Device type column |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1890 data points