Philippi, Miriam; Fernández-Méndez, Mar; Magaña-Gallegos, Edén; Eggers, Sarah Lena; Behnke, Jasmin; van Tussenbroek, Brigitta I; Hach, Philipp F; Elmer, Franziska; Buck-Wiese, Hagen; Salas-Acosta, Evelyn Raquel; Barba-Santos, María Guadalupe; Stefels, Jacqueline: Carbon fixation rates, nitrate uptake rates, particulate organic carbon (POC), nitrogen (PON) and phosphorus (POP) of Sargassum fluitans III in nutrient and temperature manipulation experiments, performed in Puerto Morelos, Mexico in July 2022 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.973666 (dataset in review)
Abstract:
To investigate the effect of different nutrient and temperature conditions on holopelagic Sargassum, six different manipulation experiments were performed in Puerto Morelos, Mexico in July 2022. Carbon fixation and nitrate uptake rates using stable isotopes (13C-bicarbonate and 15N-nitrate), and biomass elemental composition (particulate organic carbon, nitrogen and phosphorus) of Sargassum fluitans III were studied under different conditions in specialized ex-situ cultivation systems and bottle incubations. Experiment 1 tested the long-term effects (seven days), and Experiment 2 the short-term effects (two days) of different nitrate and phosphate concentrations. Experiment 4 investigated the temporal development of responses to elevated nutrient concentrations over the course of 36 hours. In Experiment 5, S. fluitans III was grown for one day under different temperatures. Experiment 6 tested the individual short-term (two days) effects of either elevated nitrate or elevated phosphate conditions. And in Experiment 8, different trace elements were provided through addition of olivine sand or volcanic ash.
Keyword(s):
References:
Garcia-Robledo, Emilio; Corzo, Alfonso; Papaspyrou, Sokratis (2014): A fast and direct spectrophotometric method for the sequential determination of nitrate and nitrite at low concentrations in small volumes. Marine Chemistry, 162, 30-36, https://doi.org/10.1016/j.marchem.2014.03.002
Grasshoff, Klaus; Kremling, Klaus; Ehrhardt, M (1999): Methods of Seawater Analysis, Third Edition. Wiley-VCH, https://doi.org/10.1002/9783527613984.fmatter
Mohr, Wiebke; Großkopf, Tobias; Wallace, Douglas WR; LaRoche, Julie (2010): Methodological Underestimation of Oceanic Nitrogen Fixation Rates. PLoS ONE, 5(9), e12583, https://doi.org/10.1371/journal.pone.0012583
Project(s):
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03F0896B: DAM CDRmare - sea4soCiety, AWI
Coverage:
Latitude: 20.868118 * Longitude: -86.866836
Date/Time Start: 2022-07-07T00:00:00 * Date/Time End: 2022-07-29T00:00:00
Event(s):
MEX22_EX1 (EX1) * Latitude: 20.868118 * Longitude: -86.866836 * Date/Time Start: 2022-07-07T00:00:00 * Date/Time End: 2022-07-14T00:00:00 * Location: Puerto Morelos, Mexico * Campaign: s4s_MEX-Land_2022 * Method/Device: Experiment (EXP) * Comment: Experiment 1: Sargassum incubated for 7 days under 5 different nutrient conditions
MEX22_EX2 (EX2) * Latitude: 20.868118 * Longitude: -86.866836 * Date/Time Start: 2022-07-14T00:00:00 * Date/Time End: 2022-07-16T00:00:00 * Location: Puerto Morelos, Mexico * Campaign: s4s_MEX-Land_2022 * Method/Device: Experiment (EXP) * Comment: Experiment 2: Sargassum incubated for 2 days under 5 different nutrient conditions
MEX22_EX4 (EX4) * Latitude: 20.868118 * Longitude: -86.866836 * Date/Time Start: 2022-07-20T00:00:00 * Date/Time End: 2022-07-23T00:00:00 * Location: Puerto Morelos, Mexico * Campaign: s4s_MEX-Land_2022 * Method/Device: Experiment (EXP) * Comment: Experiment 4: Sargassum incubated at the same nutrient conditions for varibale time
Comment:
The event location refers to where Sargassum was collected for the incubations. The experiments were performed ~85 m away under natural light conditions.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Philippi, Miriam | |||
| 2 | Type of study | Study type | Philippi, Miriam | |||
| 3 | Sample ID | Sample ID | Philippi, Miriam | |||
| 4 | Experiment | Exp | Philippi, Miriam | |||
| 5 | Treatment | Treat | Philippi, Miriam | Treatment summary: S = Sargassum, W= Water only, NIC = No Isotope addition Control, C0 = no nutrients added, C1-C4 = four different nutrient conditions, t0 = before start of the incubation, t1-t4 = four different incubation times, Temp1-Temp4 = four different incubation temperatures, N2 and N4 = two different nitrate concentrations, P2 and P4 = two different phosphate concentrations, O = Olivine addition, A = Volcano ash addition | ||
| 6 | Sampling date/time, experiment | Date/time sampling exp | Philippi, Miriam | UTC | ||
| 7 | Sampling date/time, experiment | Date/time sampling exp | Philippi, Miriam | Local | ||
| 8 | Sample type | Samp type | Philippi, Miriam | S = Sargassum; W = seawater only; t0 = beginn of the experiment, untreated | ||
| 9 | Treatment: nitrate | T:[NO3]- | µmol/l | Philippi, Miriam | Pipetting from stock solution. Nitrate addition to aimed final concentration (Experiments 1 -4 and 6) | |
| 10 | Treatment: phosphate | T:[PO4]3- | µmol/l | Philippi, Miriam | Pipetting from stock solution. Phosphate addition to aimed final concentration (Experiments 1 -4 and 6) | |
| 11 | Treatment: trace elements | T: trace elem | Philippi, Miriam | Addition of Volcano Ash, Olivine or nothing (0) (Experiment 8). 0.1 kg/L Olivine and 1 g/L Ash in sea water were shaken vigorously for 10 min, left standing for sedimentation for 20-30 minutes and afterward filtered through a GMF filter | ||
| 12 | Treatment: temperature | T:temp | °C | Philippi, Miriam | ||
| 13 | Treatment: mesocosm incubation time | T:meso inc time | days | Philippi, Miriam | ||
| 14 | Treatment: bottle incubation time | T:bot inc time | h | Philippi, Miriam | ||
| 15 | Nitrate and Nitrite | [NO3]- + [NO2]- | µmol/l | Magaña-Gallegos, Edén | Spectrophotometer; according to Garcia-Robledo et al. (2014) | Concentration of nitrate plus nitrite in the bottle |
| 16 | Phosphate | [PO4]3- | µmol/l | Magaña-Gallegos, Edén | Methods of Seawater Analysis, Third Edition (Grasshoff et al., 1999) | Concentration of phosphate in the bottle |
| 17 | Sargassum fluitans, wet mass | S. fluitans wm | g | Philippi, Miriam | Scale | Sargassum wet weight at the end of the bottle isotope incubation |
| 18 | Sargassum fluitans, dry mass | S. fluitans dm | g | Philippi, Miriam | Scale | Sargassum dry weigth at the end of the bottle isotope incubation. Dried in oven at 60°C |
| 19 | Sargassum fluitans, wet mass/dry mass ratio | S. fluitans wm/dm | Philippi, Miriam | Calculated | ||
| 20 | Sargassum fluitans, particulate organic carbon per dry mass | S. fluitans POC/dm | µg/mg | Philippi, Miriam | Isotope ratio mass spectrometer (IRMS), Thermo Scientific, Delta Plus Advantage; coupled with Elemental analyser, ThermoFisher Scientific, IsoLink EA [via ConFlo IV universal continuous flow interface] | POC1w: Particulate organic carbon content of Sargassum biomass, measured with an EA-IRMS |
| 21 | Sargassum fluitans, particulate organic carbon per dry mass | S. fluitans POC/dm | µmol/mg | Philippi, Miriam | Isotope ratio mass spectrometer (IRMS), Thermo Scientific, Delta Plus Advantage; coupled with Elemental analyser, ThermoFisher Scientific, IsoLink EA [via ConFlo IV universal continuous flow interface] | POC1: Molar particulate organic carbon content of Sargassum biomass, measured with an EA-IRMS |
| 22 | Sargassum fluitans, particulate organic carbon per dry mass | S. fluitans POC/dm | µg/mg | Eggers, Sarah Lena | Elemental analyzer, EuroVector, EuroEA 3000 | POC2w: Particulate organic carbon content of Sargassum biomass, measured with an EA |
| 23 | Sargassum fluitans, particulate organic carbon per dry mass, standard deviation | S. fluitans POC/dm std dev | ± | Eggers, Sarah Lena | Elemental analyzer, EuroVector, EuroEA 3000 | POC2w: Standard deviation of particulate organic carbon content of Sargassum biomass, measured with an EA |
| 24 | Sargassum fluitans, particulate organic carbon per dry mass | S. fluitans POC/dm | µmol/mg | Eggers, Sarah Lena | Elemental analyzer, EuroVector, EuroEA 3000 | POC2: Molar particulate organic carbon content of Sargassum biomass, measured with an EA |
| 25 | Sargassum fluitans, particulate organic nitrogen per dry mass | S. fluitans PON/dm | µg/mg | Philippi, Miriam | Isotope ratio mass spectrometer (IRMS), Thermo Scientific, Delta Plus Advantage; coupled with Elemental analyser, ThermoFisher Scientific, IsoLink EA [via ConFlo IV universal continuous flow interface] | PON1w: Particulate organic nitrogen content of Sargassum biomass, measured with an EA-IRMS |
| 26 | Sargassum fluitans, particulate organic nitrogen per dry mass | S. fluitans PON/dm | µmol/mg | Philippi, Miriam | Isotope ratio mass spectrometer (IRMS), Thermo Scientific, Delta Plus Advantage; coupled with Elemental analyser, ThermoFisher Scientific, IsoLink EA [via ConFlo IV universal continuous flow interface] | PON1: Molar particulate organic nitrogen content of Sargassum biomass, measured with an EA-IRMS |
| 27 | Sargassum fluitans, particulate organic nitrogen per dry mass | S. fluitans PON/dm | µg/mg | Eggers, Sarah Lena | Elemental analyzer, EuroVector, EuroEA 3000 | PON2w: Particulate organic nitrogen content of Sargassum biomass, measured with an EA |
| 28 | Sargassum fluitans, particulate organic nitrogen per dry mass, standard deviation | S. fluitans PON/dm std dev | ± | Eggers, Sarah Lena | Elemental analyzer, EuroVector, EuroEA 3000 | PON2w: Standard deviation of particulate organic nitrogen content of Sargassum biomass, measured with an EA |
| 29 | Sargassum fluitans, particulate organic nitrogen per dry mass | S. fluitans PON/dm | µmol/mg | Eggers, Sarah Lena | Elemental analyzer, EuroVector, EuroEA 3000 | PON2: Molar particulate organic nitrogen content of Sargassum biomass, measured with an EA |
| 30 | Sargassum fluitans, particulate organic phosphorus per dry mass | S. fluitans POP/dm | µmol/mg | Behnke, Jasmin | Spectrophotometer (SPEC) | POP: Molar particulate organic phosphorus content of Sargassum biomass. Spectrophotometric analysis of dissolved inorganic phosphate after oxidising the organic phosphorus to orthophosphate in a pressure cooker |
| 31 | Sargassum fluitans, particulate organic carbon/particulate organic nitrogen | S. fluitans POC/PON | Philippi, Miriam | Calculated | C:N ratio1: Sargassum POC1 divided by Sargassum PON1 | |
| 32 | Sargassum fluitans, particulate organic carbon/particulate organic nitrogen | S. fluitans POC/PON | Philippi, Miriam | Calculated | C:N ratio2: Sargassum POC2 divided by Sargassum PON2 | |
| 33 | Sargassum fluitans, particulate organic carbon/particulate organic phosphorus | S. fluitans POC/POP | Philippi, Miriam | Calculated | C:P ratio1: Sargassum POC1 divided by Sargassum POP | |
| 34 | Sargassum fluitans, particulate organic carbon/particulate organic phosphorus | S. fluitans POC/POP | Philippi, Miriam | Calculated | C:P ratio2: Sargassum POC2 divided by Sargassum POP | |
| 35 | Sargassum fluitans, particulate organic nitrogen/particulate organic phosphorus | S. fluitans PON/POP | Philippi, Miriam | Calculated | N:P ratio1: Sargassum PON1 divided by Sargassum POP | |
| 36 | Sargassum fluitans, particulate organic nitrogen/particulate organic phosphorus | S. fluitans PON/POP | Philippi, Miriam | Calculated | N:P ratio2: Sargassum PON2 divided by Sargassum POP | |
| 37 | Sargassum fluitans, carbon fixation rate, per dry mass | S. fluitans C fixation | µmol/mg/day | Philippi, Miriam | Calculated according to Mohr et al. (2010) | C fixation1: Sargassum carbon fixation rates calculated from the incorporation of 13C into biomass as described in Mohr et al. (2010), based on POC1. Isotopes were analyzed with the isotopic analyzer Picarro G2101-i |
| 38 | Sargassum fluitans, absolute carbon fixation rate, per dry mass | S. fluitans abs C fixation | µmol/mg | Philippi, Miriam | Calculated according to Mohr et al. (2010) | absolute C fixation1: Sargassum absolute carbon fixation rates calculated from the incorporation of 13C into biomass as described in Mohr et al. (2010), based on POC1 (Experiment 4). Isotopes were analyzed with the isotopic analyzer Picarro G2101-i |
| 39 | Sargassum fluitans, carbon fixation rate, per dry mass | S. fluitans C fixation | µmol/mg/day | Philippi, Miriam | Calculated according to Mohr et al. (2010) | C fixation2: Sargassum carbon fixation rates calculated from the incorporation of 13C into biomass as described in Mohr et al. (2010), based on POC2. Isotopes were analyzed with the isotopic analyzer Picarro G2101-i |
| 40 | Sargassum fluitans, nitrate uptake rate, per dry mass | S. fluitans [NO3]- uptake | µmol/mg/day | Philippi, Miriam | Calculated according to Mohr et al. (2010) | N uptake1: Sargassum nitrate uptake rates calculated from the incorporation of 15N into biomass as described in Mohr et al. (2010), based on PON1. Isotopes were analyzed with the isotopic analyzer Picarro G2101-i |
| 41 | Sargassum fluitans, absolute nitrate uptake rate, per dry mass | S. fluitans abs [NO3]- uptake | µmol/mg | Philippi, Miriam | Calculated according to Mohr et al. (2010) | absolute N uptake1: Sargassum absolute nitrate uptake rates calculated from the incorporation of 15N into biomass as described in Mohr et al. (2010), based on PON1 (Experiment 4). Isotopes were analyzed with the isotopic analyzer Picarro G2101-i |
| 42 | Sargassum fluitans, nitrate uptake rate, per dry mass | S. fluitans [NO3]- uptake | µmol/mg/day | Philippi, Miriam | Calculated according to Mohr et al. (2010) | N uptake2: Sargassum nitrate uptake rates calculated from the incorporation of 15N into biomass as described in Mohr et al., (2010), based on PON2. Isotopes were analyzed with the isotopic analyzer Picarro G2101-i |
| 43 | Carbon, organic, particulate | POC | µmol/l | Philippi, Miriam | Isotope ratio mass spectrometer (IRMS), Thermo Scientific, Delta Plus Advantage; coupled with Elemental analyser, ThermoFisher Scientific, IsoLink EA [via ConFlo IV universal continuous flow interface] | Molar particulate organic carbon content of biomass on GFF filter |
| 44 | Nitrogen, organic, particulate | PON | µmol/l | Philippi, Miriam | Isotope ratio mass spectrometer (IRMS), Thermo Scientific, Delta Plus Advantage; coupled with Elemental analyser, ThermoFisher Scientific, IsoLink EA [via ConFlo IV universal continuous flow interface] | Molar particulate organic nitrogen content of biomass on GFF filter |
| 45 | Carbon fixation rate | C fix | µmol/l/day | Philippi, Miriam | Calculated according to Mohr et al. (2010) | GFF filter biomass carbon fixation rates calculated from the incorporation of 13C into biomass as described in Mohr et al. (2010). Isotopes were analyzed with the isotopic analyzer Picarro G2101-i |
| 46 | Nitrate uptake rate | [NO3]- uptake | µmol/mg/day | Philippi, Miriam | Calculated according to Mohr et al. (2010) | GFF filter biomass nitrate uptake rates calculated from the incorporation of 15N into biomass as described in Mohr et al. (2010). Isotopes were analyzed with the isotopic analyzer Picarro G2101-i |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
4440 data points
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