Takeda, Shigenobu: Growth responses of Skeletonema marinoi-dohrnii complex and Chattonella marina under different nitric oxide (NO) supply rates [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972843 (dataset in review)
Abstract:
Growth responses of Skeletonema marinoi-dohrnii complex and Chattonella marina isolated from Japanese coastal waters were monitored under different nitric oxide (NO) supply rates using a variable-volume repeated fed-batch (VRFB) culture system. Culture experiments for S. marinoi-dohrnii complex were performed at three NO levels: low, medium, and high. The average NO supply rates at the low, medium, and high NO levels were 1.4, 5.4, and 28 pM/s, respectively. In the C. marina culture experiments, 2.5 pM/s NO supply rate (low-medium treatment) was used instead of 28 pM/s. In both experiments, a treatment feeding deoxygenated f/2 medium without NO was used as the control. The S. marinoi-dohrnii complex and C. marina were cultured in the VRFB culture system for 3 days. To examine the recovery from NO-induced growth inhibition, phytoplankton growth was monitored for several days after the termination of 3-day NO supply.
Supplement to:
Takeda, Shigenobu (in prep.): Growth responses of coastal phytoplankton to continuous supply of low nitric oxide concentrations during the light period. Marine Biology
References:
Guillard, R R L; Ryther, J H (1962): Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. Canadian Journal of Microbiology, 8(2), 229-239, https://doi.org/10.1139/m62-029
Funding:
Japan Society for the Promotion of Science (JSPS), grant/award no. 22K19213: Function of nitric oxide as a new growth regulator of red tide algae
Coverage:
Median Latitude: 34.457500 * Median Longitude: 134.652500 * South-bound Latitude: 34.410000 * West-bound Longitude: 134.000000 * North-bound Latitude: 34.505000 * East-bound Longitude: 135.305000
Date/Time Start: 1982-07-30T03:00:00 * Date/Time End: 1985-01-21T03:00:00
Event(s):
Chattonella (Origin of Chattonella marina isolates) * Latitude: 34.410000 * Longitude: 134.000000 * Date/Time: 1982-07-30T03:00:00 * Location: Bisan-Seto, Seto Inland Sea, Naoshima, Kagawa, Japan * Method/Device: Water sampler * Comment: Chattonella marina,
isolated by Yoshimatsu Sadaaki from water samples taken by the Kagawa Prefecture Red tide Research Institute; strain NIES-113 provided by the Microbial Culture Collection at the National Institute for Environmental Studies through the National BioResource Project of the MEXT, Japan
isolated by Yoshimatsu Sadaaki from water samples taken by the Kagawa Prefecture Red tide Research Institute; strain NIES-113 provided by the Microbial Culture Collection at the National Institute for Environmental Studies through the National BioResource Project of the MEXT, Japan
Skeletonema (Origin of S. marinoi-dohrnii complex isolates) * Latitude: 34.505000 * Longitude: 135.305000 * Date/Time: 1985-01-21T03:00:00 * Location: Off Kishiwada, Osaka Bay, Osaka, Japan * Method/Device: Water sampler * Comment: Skeletonema marinoi-dohrnii complex,
isolated by Yamochi Susumu from water samples taken by the Osaka Prefectural Fisheries Experimental Station; strain NIES-323 provided by the Microbial Culture Collection at the National Institute for Environmental Studies through the National BioResource Project of the MEXT, Japan
isolated by Yamochi Susumu from water samples taken by the Osaka Prefectural Fisheries Experimental Station; strain NIES-323 provided by the Microbial Culture Collection at the National Institute for Environmental Studies through the National BioResource Project of the MEXT, Japan
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Takeda, Shigenobu | |||
| 2 | Type of study | Study type | Takeda, Shigenobu | Variable-volume repeated fed-batch culture system (VRFB) | ||
| 3 | Date/time start, experiment | Date/time start exp | Takeda, Shigenobu | |||
| 4 | Date/time end, experiment | Date/time end exp | Takeda, Shigenobu | |||
| 5 | Species | Species | Takeda, Shigenobu | |||
| 6 | Strain | Strain | Takeda, Shigenobu | |||
| 7 | Experimental treatment | Exp treat | Takeda, Shigenobu | |||
| 8 | Treatment: nitric oxide, supply rate | T:NO supply rate | pmol/l/s | Takeda, Shigenobu | NO sensor, World Precision Instruments, ISO-NOPF200; Free-radical analyzer, World Precision Instruments, TBR4100 | |
| 9 | Treatment: feed solution, supply rate | T:feed solution supply rate | ml/min | Takeda, Shigenobu | Peristaltic pump, Masterflex, L/S 7550-50 | Nitric oxide feed solution was pumped only for 14 hours during the light period. |
| 10 | Time, incubation | T incubation | day | Takeda, Shigenobu | Variable-volume repeated fed-batch culture system (VRFB) | f/2 medium (Guillard and Ryther 1962); 20 °C; 100 ?mol/m2/s photon flux density; 14:10 light-dark cycle. |
| 11 | Cell | Cell | #/ml | Takeda, Shigenobu | Inverted microscope, Olympus, CKX-41 | |
| 12 | Growth rate | µ | 1/day | Takeda, Shigenobu | Calculated | Specific growth rates from Day 1 to 3 of incubation; Calculated from the slope of the exponential fitting of the relationship between cell number and incubation duration. |
| 13 | Fluorescence, in vivo | Fluorescence in vivo | RFU | Takeda, Shigenobu | Laboratory fluorometer, Turner, Trilogy; with a chlorophyll in-vivo module |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
684 data points