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Takeda, Shigenobu: Growth responses of Skeletonema marinoi-dohrnii complex and Chattonella marina under different nitric oxide (NO) supply rates [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972843 (dataset in review)

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Abstract:
Growth responses of Skeletonema marinoi-dohrnii complex and Chattonella marina isolated from Japanese coastal waters were monitored under different nitric oxide (NO) supply rates using a variable-volume repeated fed-batch (VRFB) culture system. Culture experiments for S. marinoi-dohrnii complex were performed at three NO levels: low, medium, and high. The average NO supply rates at the low, medium, and high NO levels were 1.4, 5.4, and 28 pM/s, respectively. In the C. marina culture experiments, 2.5 pM/s NO supply rate (low-medium treatment) was used instead of 28 pM/s. In both experiments, a treatment feeding deoxygenated f/2 medium without NO was used as the control. The S. marinoi-dohrnii complex and C. marina were cultured in the VRFB culture system for 3 days. To examine the recovery from NO-induced growth inhibition, phytoplankton growth was monitored for several days after the termination of 3-day NO supply.
Keyword(s):
Laboratory experiment; nitric oxide; Phytoplankton
Supplement to:
Takeda, Shigenobu (in prep.): Growth responses of coastal phytoplankton to continuous supply of low nitric oxide concentrations during the light period. Marine Biology
References:
Guillard, R R L; Ryther, J H (1962): Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. Canadian Journal of Microbiology, 8(2), 229-239, https://doi.org/10.1139/m62-029
Funding:
Japan Society for the Promotion of Science (JSPS), grant/award no. 22K19213: Function of nitric oxide as a new growth regulator of red tide algae
Coverage:
Median Latitude: 34.457500 * Median Longitude: 134.652500 * South-bound Latitude: 34.410000 * West-bound Longitude: 134.000000 * North-bound Latitude: 34.505000 * East-bound Longitude: 135.305000
Date/Time Start: 1982-07-30T03:00:00 * Date/Time End: 1985-01-21T03:00:00
Event(s):
Chattonella (Origin of Chattonella marina isolates)  * Latitude: 34.410000 * Longitude: 134.000000 * Date/Time: 1982-07-30T03:00:00 * Location: Bisan-Seto, Seto Inland Sea, Naoshima, Kagawa, Japan * Method/Device: Water sampler * Comment: Chattonella marina,
isolated by Yoshimatsu Sadaaki from water samples taken by the Kagawa Prefecture Red tide Research Institute; strain NIES-113 provided by the Microbial Culture Collection at the National Institute for Environmental Studies through the National BioResource Project of the MEXT, Japan
Skeletonema (Origin of S. marinoi-dohrnii complex isolates)  * Latitude: 34.505000 * Longitude: 135.305000 * Date/Time: 1985-01-21T03:00:00 * Location: Off Kishiwada, Osaka Bay, Osaka, Japan * Method/Device: Water sampler * Comment: Skeletonema marinoi-dohrnii complex,
isolated by Yamochi Susumu from water samples taken by the Osaka Prefectural Fisheries Experimental Station; strain NIES-323 provided by the Microbial Culture Collection at the National Institute for Environmental Studies through the National BioResource Project of the MEXT, Japan
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Event labelEventTakeda, Shigenobu
2 Type of studyStudy typeTakeda, ShigenobuVariable-volume repeated fed-batch culture system (VRFB)
3 Date/time start, experimentDate/time start expTakeda, Shigenobu
4 Date/time end, experimentDate/time end expTakeda, Shigenobu
5 SpeciesSpeciesTakeda, Shigenobu
6 StrainStrainTakeda, Shigenobu
7 Experimental treatmentExp treatTakeda, Shigenobu
8 Treatment: nitric oxide, supply rateT:NO supply ratepmol/l/sTakeda, ShigenobuNO sensor, World Precision Instruments, ISO-NOPF200; Free-radical analyzer, World Precision Instruments, TBR4100
9 Treatment: feed solution, supply rateT:feed solution supply rateml/minTakeda, ShigenobuPeristaltic pump, Masterflex, L/S 7550-50Nitric oxide feed solution was pumped only for 14 hours during the light period.
10 Time, incubationT incubationdayTakeda, ShigenobuVariable-volume repeated fed-batch culture system (VRFB)f/2 medium (Guillard and Ryther 1962); 20 °C; 100 ?mol/m2/s photon flux density; 14:10 light-dark cycle.
11 CellCell#/mlTakeda, ShigenobuInverted microscope, Olympus, CKX-41
12 Growth rateµ1/dayTakeda, ShigenobuCalculatedSpecific growth rates from Day 1 to 3 of incubation; Calculated from the slope of the exponential fitting of the relationship between cell number and incubation duration.
13 Fluorescence, in vivoFluorescence in vivoRFUTakeda, ShigenobuLaboratory fluorometer, Turner, Trilogy; with a chlorophyll in-vivo module
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
684 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Event		2 
Study type		3 
Date/time start exp		4 
Date/time end exp		5 
Species		6 
Strain		7 
Exp treat		8 
T:NO supply rate [pmol/l/s]		9 
T:feed solution supply rate [ml/min]		10 
T incubation [day]		11 
Cell [#/ml]		12 
µ [1/day]		13 
Fluorescence in vivo [RFU]
Skeletonema Laboratory experiment2023-01-27T22:30:002023-01-30T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Control0.00.0092077
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-01-30T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Control0.00.517620508
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-01-30T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Control0.00.52315002630
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-01-30T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Control0.00.531720001.56013800
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Low Nitric oxide supply0.00.00103096
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Low Nitric oxide supply1.40.516150492
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Low Nitric oxide supply1.40.52255001890
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Low Nitric oxide supply1.40.531120001.4509170
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Low Nitric oxide supply0.00.0430600
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Low Nitric oxide supply0.00.05100000
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Low Nitric oxide supply0.00.06133000
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Low Nitric oxide supply0.00.07261000
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Low Nitric oxide supply0.00.08543000
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Medium Nitric oxide supply0.00.00129093
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Medium Nitric oxide supply5.40.512010172
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Medium Nitric oxide supply5.40.522880228
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Medium Nitric oxide supply5.40.5365100.588462
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Medium Nitric oxide supply0.00.04843
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Medium Nitric oxide supply0.00.054700
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Medium Nitric oxide supply0.00.0624300
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Medium Nitric oxide supply0.00.0799600
SkeletonemaLaboratory experiment2023-01-30T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323Medium Nitric oxide supply0.00.08250000
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply0.00.001020104
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply28.00.51144072
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply28.00.52191082
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply28.00.5318700.12951
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply0.00.0424
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply0.00.0587
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply0.00.061030
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply0.00.076840
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply0.00.0836800
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply0.00.0998300
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply0.00.010189000
SkeletonemaLaboratory experiment2023-01-27T22:30:002023-02-07T22:30:00Skeletonema marinoi-dohrnii complexNIES-323High Nitric oxide supply0.00.011487000
Chattonella Laboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Control0.00.0014
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Control0.00.5125
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Control0.00.5241
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Control0.00.53750.552
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Control0.00.04137
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Control0.00.05201
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Control0.00.06339
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Control0.00.07501
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low Nitric oxide supply0.00.0021
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low Nitric oxide supply1.40.5119
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low Nitric oxide supply1.40.5220
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low Nitric oxide supply1.40.5318-0.033
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low Nitric oxide supply0.00.0433
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low Nitric oxide supply0.00.0537
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low Nitric oxide supply0.00.0640
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low Nitric oxide supply0.00.0761
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low-Medium Nitric oxide supply0.00.0021
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low-Medium Nitric oxide supply2.50.5120
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low-Medium Nitric oxide supply2.50.5220
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low-Medium Nitric oxide supply2.50.53200.004
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low-Medium Nitric oxide supply0.00.0422
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low-Medium Nitric oxide supply0.00.0531
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low-Medium Nitric oxide supply0.00.0639
ChattonellaLaboratory experiment2023-03-04T22:30:002023-03-11T22:30:00Chattonella marinaNIES-113Low-Medium Nitric oxide supply0.00.0752
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Medium Nitric oxide supply0.00.0013
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Medium Nitric oxide supply5.40.5118
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Medium Nitric oxide supply5.40.5211
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Medium Nitric oxide supply5.40.535-0.594
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Medium Nitric oxide supply0.00.047
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Medium Nitric oxide supply0.00.0516
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Medium Nitric oxide supply0.00.0623
ChattonellaLaboratory experiment2023-02-17T22:30:002023-02-24T22:30:00Chattonella marinaNIES-113Medium Nitric oxide supply0.00.0745