Citation:

PANGAEA.972789 (DOI registration in progress)

Name: Impact of climate change on the kelp Laminaria digitata: Biochemical and physiological raw data of a long-term experiment simulating Artic winter warming
Published: 2024
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Arctic Amplification; C:N; Fv/Fm; laminarin; Mannitol; pigments; polar night

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Abstract:

Global warming affects the Arctic much faster than the rest of the globe, with the highest amplitude of temperature rise occurring during the Polar Night. To determine the stress resistance of the ecosystem-engineering kelp Laminaria digitata against Arctic winter warming, non-meristematic discs of adult sporophytes from Porsangerfjorden (Finnmark, Norway) were kept in total darkness at 0°C and 5°C over a period of three months. The maximum photosynthetic quantum yield of photosystem II (Fv/Fm; Imaging-PAM, Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) was monitored once a week. For monitoring of potential growth, the size (area) of the algal discs was photographed every two weeks and analyzed with ImageJ (Version 1.52a). Every four weeks, subsamples for monitoring the dry weight and for the biochemical analyses were taken. Laminarin content was determined following via enzymatic digestion. C:N ratio was analyzed with an Elementar Analyzer. Mannitol concentration was analyzed using a HPLC. Absolut pigment concentrations were also analyzed using a HPLC and pool sizes, the de-epoxidation state of the xanthophyll cycle (DPS), and the ratios calculated afterwards.

Keyword(s):

Arctic Amplification; C:N; Fv/Fm; laminarin; Mannitol; pigments; polar night

Supplement to:

Trautmann, Moritz; Bartsch, Inka; Bligh, Margot; Buck-Wiese, Hagen; Hehemann, Jan-Hendrik; Niedzwiedz, Sarina; Plag, Niklas; Shan, Tifeng; Bischof, Kai; Diehl, Nora (accepted): Impact of climate change on the kelp Laminaria digitata - Simulated Arctic winter warming.

Project(s):

Arctic Biodiversity & Livelihoods (FACE-IT)

Coverage:

Median Latitude: 70.469967 * Median Longitude: 25.636167 * South-bound Latitude: 70.411100 * West-bound Longitude: 25.537500 * North-bound Latitude: 70.507200 * East-bound Longitude: 25.708300

Date/Time Start: 2022-06-12T00:00:00 * Date/Time End: 2022-06-12T00:00:00

Event(s):

Porsangerfjorden_1 * Latitude: 70.411100 * Longitude: 25.537500 * Date/Time: 2022-06-12T00:00:00 * Location: Porsangerfjorden, Norway * Method/Device: Algae Grabber via boat

Porsangerfjorden_2 * Latitude: 70.491600 * Longitude: 25.662700 * Date/Time: 2022-06-12T00:00:00 * Location: Porsangerfjorden, Norway * Method/Device: Algae Grabber via boat

Porsangerfjorden_3 * Latitude: 70.507200 * Longitude: 25.708300 * Date/Time: 2022-06-12T00:00:00 * Location: Porsangerfjorden, Norway * Method/Device: Algae Grabber via boat

Comment:

Treatment details

----------------------------

Laminaria digitata samples from the Porsangerfjorden, Finnmark, Norway

0°C and 5°C, total darkness for 3 months

w0 = week 0 (start)

w4 = week 4

w8 = week 8

w12 = week 12 (end)

set-up:

2-L aerated Kautex bottles, 1/40 PES, medium changed 2x/week

Sample: These values are used to differentiate multiple similar samples within the same replicate. They do not denote a relationship between weeks. E.g. Sample #3 from week 0 (w0) of Replicate 2, is not necessarily a sample of the same organism for Sample #3 from week 1 (w1) of Replicate 2. Meaning that Samples should not be taken as time series of measures of the same organism through time.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Diehl, Nora
2	Date/time start, experiment	Date/time start exp		Diehl, Nora
3	Date/time end, experiment	Date/time end exp		Diehl, Nora
4	Species	Species		Diehl, Nora
5	Species, unique identification	Species UID		Diehl, Nora
6	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Diehl, Nora
7	Species, unique identification (URI)	Species UID (URI)		Diehl, Nora
8	Family	Family		Diehl, Nora
9	Location	Location		Diehl, Nora
10	Treatment: light:dark cycle	T:L:D	hh:hh	Diehl, Nora
11	Treatment: temperature	T:temp	°C	Diehl, Nora
12	Replicate	Repl		Diehl, Nora
13	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w0
14	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w1
15	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w2
16	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w3
17	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w4
18	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w5
19	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w6
20	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w7
21	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w8
22	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w9
23	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w10
24	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w11
25	Maximum quantum yield of photosystem II	Fv/Fm		Diehl, Nora	Chlorophyll Fluorescence System (I-PAM), Walz, Imaging-PAM fluorometer	w12
26	Area	Area	cm²	Diehl, Nora	ImageJ (Version 1.52a)	w0
27	Area	Area	cm²	Diehl, Nora	ImageJ (Version 1.52a)	w2
28	Area	Area	cm²	Diehl, Nora	ImageJ (Version 1.52a)	w4
29	Area	Area	cm²	Diehl, Nora	ImageJ (Version 1.52a)	w6
30	Area	Area	cm²	Diehl, Nora	ImageJ (Version 1.52a)	w8
31	Area	Area	cm²	Diehl, Nora	ImageJ (Version 1.52a)	w10
32	Area	Area	cm²	Diehl, Nora	ImageJ (Version 1.52a)	w12
33	Biomass, dry mass	Biom dm	g	Diehl, Nora	Precision Balance, Sartorius, MC 21S	w0
34	Biomass, dry mass	Biom dm	g	Diehl, Nora	Precision Balance, Sartorius, MC 21S	w4
35	Biomass, dry mass	Biom dm	g	Diehl, Nora	Precision Balance, Sartorius, MC 21S	w8
36	Biomass, dry mass	Biom dm	g	Diehl, Nora	Precision Balance, Sartorius, MC 21S	w12
37	Laminarin, per dry mass	Laminarin/dm	mg/g	Diehl, Nora	Enzymatic digestion	w0
38	Laminarin, per dry mass	Laminarin/dm	mg/g	Diehl, Nora	Enzymatic digestion	w4
39	Laminarin, per dry mass	Laminarin/dm	mg/g	Diehl, Nora	Enzymatic digestion	w8
40	Laminarin, per dry mass	Laminarin/dm	mg/g	Diehl, Nora	Enzymatic digestion	w12
41	Mannitol, per dry mass	Mannitol/dm	mg/g	Diehl, Nora	High performance liquid chromatography (HPLC), Agilent Technologies, 1200 Series; coupled with Aminex Fast Carbohydrate analysis pillar HPAP (100 х 7,8 mm, 9 μ), BioRad front pillar: Phenomenex, Carbo-Pb2+ 4 x 3.00 mm i.d	w0
42	Mannitol, per dry mass	Mannitol/dm	mg/g	Diehl, Nora	High performance liquid chromatography (HPLC), Agilent Technologies, 1200 Series; coupled with Aminex Fast Carbohydrate analysis pillar HPAP (100 х 7,8 mm, 9 μ), BioRad front pillar: Phenomenex, Carbo-Pb2+ 4 x 3.00 mm i.d	w4
43	Mannitol, per dry mass	Mannitol/dm	mg/g	Diehl, Nora	High performance liquid chromatography (HPLC), Agilent Technologies, 1200 Series; coupled with Aminex Fast Carbohydrate analysis pillar HPAP (100 х 7,8 mm, 9 μ), BioRad front pillar: Phenomenex, Carbo-Pb2+ 4 x 3.00 mm i.d	w8
44	Mannitol, per dry mass	Mannitol/dm	mg/g	Diehl, Nora	High performance liquid chromatography (HPLC), Agilent Technologies, 1200 Series; coupled with Aminex Fast Carbohydrate analysis pillar HPAP (100 х 7,8 mm, 9 μ), BioRad front pillar: Phenomenex, Carbo-Pb2+ 4 x 3.00 mm i.d	w12
45	Carbon, total, per dry mass	TC/dm	mg/g	Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w0
46	Carbon, total, per dry mass	TC/dm	mg/g	Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w4
47	Carbon, total, per dry mass	TC/dm	mg/g	Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w8
48	Carbon, total, per dry mass	TC/dm	mg/g	Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w12
49	Nitrogen, total, per dry mass	TN/dm	mg/g	Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w0
50	Nitrogen, total, per dry mass	TN/dm	mg/g	Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w4
51	Nitrogen, total, per dry mass	TN/dm	mg/g	Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w8
52	Nitrogen, total, per dry mass	TN/dm	mg/g	Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w12
53	Carbon/Nitrogen ratio	C/N		Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w0
54	Carbon/Nitrogen ratio	C/N		Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w4
55	Carbon/Nitrogen ratio	C/N		Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w8
56	Carbon/Nitrogen ratio	C/N		Diehl, Nora	CHSN Elemental Analyser, Elementar Analysen systeme GmbH, Germany, vario EL III	w12
57	Chlorophyll a, per dry mass	Chl a/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w0
58	Chlorophyll a, per dry mass	Chl a/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w4
59	Chlorophyll a, per dry mass	Chl a/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w8
60	Chlorophyll a, per dry mass	Chl a/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w12
61	Fucoxanthin, per dry mass	Fuco dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w0
62	Fucoxanthin, per dry mass	Fuco dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w4
63	Fucoxanthin, per dry mass	Fuco dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w8
64	Fucoxanthin, per dry mass	Fuco dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w12
65	Chlorophyll c2, per dry mass	Chl c2/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w0
66	Chlorophyll c2, per dry mass	Chl c2/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w4
67	Chlorophyll c2, per dry mass	Chl c2/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w8
68	Chlorophyll c2, per dry mass	Chl c2/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w12
69	beta-Carotene, per dry mass	b-Car/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w0
70	beta-Carotene, per dry mass	b-Car/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w4
71	beta-Carotene, per dry mass	b-Car/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w8
72	beta-Carotene, per dry mass	b-Car/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w12
73	Violaxanthin, per dry mass	Viola/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w0
74	Violaxanthin, per dry mass	Viola/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w4
75	Violaxanthin, per dry mass	Viola/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w8
76	Violaxanthin, per dry mass	Viola/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w12
77	Antheraxanthin, per dry mass	Anthera/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w0
78	Antheraxanthin, per dry mass	Anthera/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w4
79	Antheraxanthin, per dry mass	Anthera/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w8
80	Antheraxanthin, per dry mass	Anthera/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w12
81	Zeaxanthin, per dry mass	Zea/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w0
82	Zeaxanthin, per dry mass	Zea/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w4
83	Zeaxanthin, per dry mass	Zea/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w8
84	Zeaxanthin, per dry mass	Zea/dm	µg/g	Diehl, Nora	High performance liquid chromatography (HPLC), VWR Hitachi International, LaChromElite® system, L-2200 autosampler [cooled], DA-detector L-2450, [Pillar: Spherisorb® ODS-2 (250 × 4.6 mm, 5 μm), Waters]	w12

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

5049 data points

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