Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Binder, Franziska Isabell; Bünning, Tobias Hartwig; Schuster, Romina Marietta; Marx, Ute; Strehse, Jennifer Susanne; Maser, Edmund; Brenner, Matthias: Uptake, potential biotransformation and depuration of TNT in exposed blue mussels (Mytilus edulis L.) [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972751 (dataset in review)

Show MapGoogle Earth

Abstract:
The uptake, potential biotransformation and depuration time of dissolved TNT in mussels were investigated by two laboratory experiments using common blue mussels (Mytilus edulis) originating from the Island of Sylt/German North Sea. The first experiment was conducted in July 2020 and the second in February 2022 in the laboratory of the Alfred Wegener Institute, Bremerhaven. Mussels were exposed to different TNT concentrations over defined periods of time (first experiment: 48 hours exposure to 4 different TNT concentrations; 0 mg/l, 0.625 mg/l, 1.25 mg/l and 2.5 mg/l, second experiment: 24 hours exposure to 0 mg/l and 5 mg/l deuterated TNT) followed by recovery phase in clean artificial seawater (first experiment: 60 hours recovery, second experiment: 12 hours recovery). Aquaria water and mussel tissues were sampled from both exposure and recovery phases and analysed for TNT and its metabolites for treatment and control groups. The analysis of TNT and its metabolites 2-amino-4,6-dinitrotoluene (2-ADNT), 4-amino-2,6-dinitrotoluene (4-ADNT) and 2,4-diamino-6-nitrotoluene (2,4-DANT) were conducted by using GC-MS/MS techniques.
Keyword(s):
2-ADNT; 4-ADNT; exposure; Laboratory experiment; metabolites; Mytilus edulis; TNT
Supplement to:
Binder, Franziska Isabell; Schuster, Romina Marietta; Bünning, Tobias Hartwig; Strehse, Jennifer Susanne; Maser, Edmund; Brenner, Matthias (in review): Depuration kinetics of TNT and its metabolites in exposed blue mussels (Mytilus edulis, L.). Toxics
Project(s):
North Sea Wrecks (NSW)
Coverage:
Latitude: 55.022500 * Longitude: 8.441333
Date/Time Start: 2020-07-14T10:00:00 * Date/Time End: 2022-01-23T10:00:00
Event(s):
M_edulis_Exp_February22 (Exp_February22) * Latitude: 55.022500 * Longitude: 8.441333 * Date/Time: 2022-01-23T10:00:00 * Location: Sylt, North Sea * Method/Device: Sampling by hand (HAND) * Comment: Collection site of Mytilus edulis for experiment 2
M_edulis_Exp_July20 (Exp_July20) * Latitude: 55.022500 * Longitude: 8.441333 * Date/Time: 2020-07-14T10:00:00 * Location: Sylt, North Sea * Method/Device: Sampling by hand (HAND) * Comment: Collection site of Mytilus edulis for experiment 1
Comment:
#0 = below detection limit, empty cells = not determined
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeBinder, Franziska Isabell
2ExperimentExpBinder, Franziska Isabell
3Experiment numberExp noBinder, Franziska Isabell
4Date/time start, experimentDate/time start expBinder, Franziska Isabell
5Sampling date/time, experimentDate/time sampling expBinder, Franziska Isabell
6Phase descriptionPhase descrBinder, Franziska IsabellExposure phase
7Incubation durationInc durhBinder, Franziska IsabellTime of exposure to different TNT concentrations
8Recovery timeRecov timehBinder, Franziska IsabellTime after exposure to experimental treatment (= Recovery)
9Experiment durationExp durationhBinder, Franziska IsabellTime after start of experiment
10Species, unique identificationSpecies UIDBinder, Franziska Isabell
11Species, unique identification (URI)Species UID (URI)Binder, Franziska Isabell
12Species, unique identification (Semantic URI)Species UID (Semantic URI)Binder, Franziska Isabell
13Experimental treatmentExp treatBinder, Franziska IsabellAcclimatisation; exposition to different TNT concentrations; controls
14Treatment: temperatureT:temp°CBinder, Franziska IsabellClimate chamber
15Treatment: salinityT:salBinder, Franziska IsabellConductivity meter, WTW LF330, probe TetraCon 325
16Aquarium numberAquariumBinder, Franziska Isabell
17Condition indexCIBinder, Franziska IsabellCalculatedCalculated as: (soft tissue mass [g] / shell mass [g]) * 100
18Mytilus edulis, soft tissue, 2,4,6-trinitrotoluene, per wet massM. edulis soft tis TNT wmng/gBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
19Mytilus edulis, soft tissue, 2-amino-4,6-dinitrotoluene, per wet massM. edulis soft tis 2-ADNT wmng/gBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
20Mytilus edulis, soft tissue, 4-amino-2,6-dinitrotoluene, per wet massM. edulis soft tis 4-ADNT wmng/gBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
21Mytilus edulis, soft tissue, 2,4-diamino-6-nitrotoluene, per wet massM. edulis soft tis 2,4-DANT wmng/gBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
222,4,6-trinitrotolueneTNTmg/lBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
232-amino-4,6-dinitrotoluene2-ADNTmg/lBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
244-amino-2,6-dinitrotoluene4-ADNTmg/lBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
25Mytilus edulis, soft tissue, 2,4,6-trinitrotoluene-d5, per wet massM. edulis soft tis TNT-d5 wmng/gBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
26Mytilus edulis, soft tissue, 2-amino-4,6-dinitrotoluene-d5, per wet massM. edulis soft tis 2-ADNT-d5 wmng/gBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
27Mytilus edulis, soft tissue, 4-amino-2,6-dinitrotoluene-d5, per wet massM. edulis soft tis 4-ADNT-d5 wmng/gBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
282,4,6-trinitrotoluene-d5TNT-d5mg/lBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
292-amino-4,6-dinitrotoluene-d52-ADNT-d5mg/lBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
304-amino-2,6-dinitrotoluene-d54-ADNT-d5mg/lBünning, Tobias HartwigGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
4772 data points

Download Data (login required; moratorium until 2025-09-26)

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML (shows only first 2000 rows)