Devresse, Quentin; Becker, Kevin W; Engel, Anja (2024): Particulate amino acid and carbohydrates and particulate organic carbon and nitrogen in suspended and sinking organic matter from METEOR cruise M160 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.972140
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Published: 2024-12-18 • DOI registered: 2025-01-16
Abstract:
We collected in situ marine snow aggregates with a Marine Snow Catcher (MSC). The MSC allows studying the composition of suspended and fast-sinking organic matter from intact in situ formed aggregates (Riley et al. 2012). After deployment, the MSC was left vertically on deck for three hours, seawater containing suspended organic matter was collected from the top part of the MSC and fast-sinking organic matter was picked manually from the MSC base. The MSC was deployed within the mixed layer depth. Duplicate samples for particulate organic carbon (POC) and organic nitrogen (PON) were filtered onto pre-combusted (450 ◦C for 5 h) Whatman GF/F filters (25 mm, 0.7 µm) under low pressure (< 200 mbar). Filters were frozen at −20 °C and stored until analysis. Prior to analysis, filters were acid-fumed (37% HCl for 24 h) in order to remove inorganic carbon and dried at 40°C for 24 h. Subsequently, filters were wrapped in tin cups (8×8×15 mm), combusted and analyzed according to Sharp (1974) using an elemental analyzer (Euro EA). We determined high-molecular-weight (>1 kDa) particulate combined carbohydrates (pCCHO) based on Engel and Händel (2011) and particulate hydrolysable amino acids (pHAA) based on Lindroth and Mopper (1979) and Dittmar et al., (2009). The analysis of pCCHO detected 11 monomers: and the pHAA analysis classified 13 monomers. The calculations for the carbon and nitrogen contents of pCCHO and pHAA were based on carbon and nitrogen atoms contained in the identified monomers.
Related to:
Dittmar, Thorsten; Cherrier, Jennifer; Ludwichowski, Kai-Uwe (2009): The Analysis of Amino Acids in Seawater. In: Wurl, O (eds.), Practical Guidelines for the Analysis of Seawater, CRC Press, https://doi.org/10.1201/9781420073072
Engel, Anja; Händel, Nicole; Wohlers, J; Lunau, Mirko; Grossart, Hans-Peter; Sommer, Ulrich; Riebesell, Ulf (2011): Effects of sea surface warming on the production and composition of dissolved organic matter during phytoplankton blooms: results from a mesocosm study. Journal of Plankton Research, 33(3), 357-372, https://doi.org/10.1093/plankt/fbq122
Lindroth, Peter; Mopper, Kenneth (1979): High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde. Analytical Chemistry, 51(11), 1667-1674, https://doi.org/10.1021/ac50047a019
Riley, J S; Sanders, Richard J; Marsay, Christopher M; Le Moigne, Frédéric A C; Achterberg, Eric Pieter; Poulton, Alex J (2012): The relative contribution of fast and slow sinking particles to ocean carbon export. Global Biogeochemical Cycles, 26(1), GB1026, https://doi.org/10.1029/2011GB004085
Project(s):
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03F0815A: Küstennahe Auftriebsgebiete REEBUS: Physikalisch-biogeochemische sowie pelagisch-benthische Kopplung in Wirbeln vor Westafrika
Coverage:
Median Latitude: 16.314136 * Median Longitude: -23.009749 * South-bound Latitude: 14.483320 * West-bound Longitude: -25.778870 * North-bound Latitude: 18.609650 * East-bound Longitude: -20.409030
Date/Time Start: 2019-11-25T20:25:00 * Date/Time End: 2019-12-16T07:40:00
Minimum DEPTH, water: 20 m * Maximum DEPTH, water: 60 m
Event(s):
M160_13-1 * Latitude: 14.483320 * Longitude: -25.049770 * Date/Time: 2019-11-25T20:25:00 * Elevation: -4297.0 m * Location: North Atlantic Ocean * Campaign: M160 * Basis: Meteor (1986) * Method/Device: Marine snow catcher (MSC)
M160_14-1 * Latitude: 14.483330 * Longitude: -25.049730 * Date/Time: 2019-11-25T20:36:00 * Elevation: -4292.0 m * Location: North Atlantic Ocean * Campaign: M160 * Basis: Meteor (1986) * Method/Device: Marine snow catcher (MSC)
M160_53-1 * Latitude: 17.799370 * Longitude: -20.600370 * Date/Time: 2019-11-30T18:55:00 * Elevation: -3139.0 m * Location: North Atlantic Ocean * Campaign: M160 * Basis: Meteor (1986) * Method/Device: Marine snow catcher (MSC)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Engel, Anja | |||
| 2 | Station label | Station | Engel, Anja | |||
| 3 | LATITUDE | Latitude | Engel, Anja | Geocode | ||
| 4 | LONGITUDE | Longitude | Engel, Anja | Geocode | ||
| 5 | Date/time start | Date/time start | Engel, Anja | deployed station 1 | ||
| 6 | Date/time end | Date/time end | Engel, Anja | on deck station 1 | ||
| 7 | Date/time start | Date/time start | Engel, Anja | deployed station 2 | ||
| 8 | Date/time end | Date/time end | Engel, Anja | on deck station 2 | ||
| 9 | DEPTH, water | Depth water | m | Engel, Anja | Geocode | |
| 10 | Nitrogen, organic, particulate | PON | µmol/l | Engel, Anja | suspended | |
| 11 | Carbon, organic, particulate | POC | µmol/l | Engel, Anja | suspended | |
| 12 | Aspartic acid and Asparagine | AsX | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 13 | Glutamine and Glutamic acid | GlX | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 14 | Serine | Ser | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 15 | Glycine | Gly | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 16 | Threonine | Thr | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 17 | Arginine | Arg | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 18 | Alanine | Ala | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 19 | gamma-Aminobutyric acid | g-ABA | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 20 | Tyrosine | Tyr | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 21 | Valine | Val | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 22 | Isoleucine | Ile | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 23 | Phenylalanine | Phe | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 24 | Leucine | Leu | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | suspended |
| 25 | Amino acids | AA | µmol/l | Engel, Anja | suspended; SUM | |
| 26 | Amino acids | AA | µmol/l | Engel, Anja | suspended; SUM C | |
| 27 | Amino acids | AA | µmol/l | Engel, Anja | suspended; SUM N | |
| 28 | Fucose | Fuc | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 29 | Rhamnose | Rha | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 30 | Galactosamine | GalN | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 31 | Arabinose | Ara | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 32 | Glucosamine | GlcN | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 33 | Galactose | Gal | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 34 | Glucose | Glc | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 35 | Mannose/Xylose | Man/Xyl | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 36 | Gluconic acid | GlucAc | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 37 | Muramic acid | Mur | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 38 | Galacturonic acid | GalA | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 39 | Glucuronic acid | GlcA | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | suspended |
| 40 | Carbohydrates, particulate combined | PCCHO | µmol/l | Engel, Anja | suspended | |
| 41 | Nitrogen, organic, particulate | PON | µmol/l | Engel, Anja | fast-sinking | |
| 42 | Carbon, organic, particulate | POC | µmol/l | Engel, Anja | fast-sinking | |
| 43 | Aspartic acid and Asparagine | AsX | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 44 | Glutamine and Glutamic acid | GlX | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 45 | Serine | Ser | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 46 | Glycine | Gly | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 47 | Threonine | Thr | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 48 | Arginine | Arg | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 49 | Alanine | Ala | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 50 | gamma-Aminobutyric acid | g-ABA | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 51 | Tyrosine | Tyr | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 52 | Valine | Val | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 53 | Isoleucine | Ile | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 54 | Phenylalanine | Phe | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 55 | Leucine | Leu | µmol/l | Engel, Anja | High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260 | fast-sinking |
| 56 | Amino acids | AA | µmol/l | Engel, Anja | fast-sinking; SUM | |
| 57 | Amino acids | AA | µmol/l | Engel, Anja | fast-sinking; SUM C | |
| 58 | Amino acids | AA | µmol/l | Engel, Anja | fast-sinking; SUM N | |
| 59 | Fucose | Fuc | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 60 | Rhamnose | Rha | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 61 | Galactosamine | GalN | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 62 | Arabinose | Ara | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 63 | Glucosamine | GlcN | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 64 | Galactose | Gal | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 65 | Glucose | Glc | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 66 | Mannose/Xylose | Man/Xyl | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 67 | Gluconic acid | GlucAc | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 68 | Muramic acid | Mur | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 69 | Galacturonic acid | GalA | µmol/l | Engel, Anja | High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000 | fast-sinking |
| 70 | Carbohydrates, particulate combined | PCCHO | µmol/l | Engel, Anja | fast-sinking | |
| 71 | Carbohydrates, particulate combined, Nitrogen | PCCHO-N | µmol/l | Engel, Anja | fast-sinking | |
| 72 | Carbohydrates, particulate combined, Carbon | PCCHO-C | µmol/l | Engel, Anja | fast-sinking | |
| 73 | Carbohydrates, particulate combined, Carbon | PCCHO-C | µmol/l | Engel, Anja | suspended | |
| 74 | Carbohydrates, particulate combined, Nitrogen | PCCHO-N | µmol/l | Engel, Anja | suspended |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
560 data points