Gutekunst, Cordula Nina; Liebner, Susanne; Jenner, Anna-Kathrina; Racasa, Erwin Don; Knorr, Klaus-Holger; Anthony, Sara E; Pönisch, Daniel Lars; Böttcher, Michael Ernst; Janssen, Manon; Kallmeyer, Jens; Koebsch, Franziska; Rehder, Gregor; Jurasinski, Gerald (2024): Absolute abundances of methane- and sulfate-cycling microorganisms, pore water gas concentrations and stable carbon isotopes (Table 1) [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972115 (DOI registration in progress), In: Gutekunst, CN et al. (2024): Brackish water rewetting of a temperate coastal peatland in NE Germany: Effects on Biogeochemistry, Microorganisms and Greenhouse gas emissions [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.972114
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Abstract:
Soil cores for microbial, dissolved gas concentrations and isotopic analysis were taken using a Russian type peat corer (De Vleeschouwer et al. 2010) before and after rewetting. Each time, we took duplicates at stations 1-8 for this rather labor-intensive process and divided the core into four depth sections: surface, 5–20, 20–40 and 40–50 cm. Subsamples for dissolved gases and stable carbon isotope analyses were taken with tip-cut syringes with a distinct volume of 3 ml (Omnifix, Braun, Bad Arolsen, Germany) and immediately placed into NaCl-saturated vials (20 ml, Agilent Technologies, 5182-0837, Santa Clara, USA) leaving no headspace and closed gas-tight using rubber stoppers and metal crimpers (both: diameter 20 mm, Glasgerätebau Ochs, Bovenden, Germany).
Keyword(s):
References:
De Vleeschouwer, Francois; Chambers, Frank M; Swindles, Graeme T (2010): Coring and sub-sampling of peatlands for palaeoenvironmental research. Mires and Peat, 7, 1, http://www.mires-and-peat.net/pages/volumes/map07/map0701.php
Funding:
German Research Foundation (DFG), grant/award no. 240942083: DFG Research Training Group Baltic TRANSCOAST
Coverage:
Median Latitude: 54.370653 * Median Longitude: 13.244863 * South-bound Latitude: 54.370461 * West-bound Longitude: 13.244600 * North-bound Latitude: 54.371100 * East-bound Longitude: 13.245037
Date/Time Start: 2019-06-19T00:00:00 * Date/Time End: 2020-08-14T00:00:00
Minimum DEPTH, soil: 0.00 m * Maximum DEPTH, soil: 0.45 m
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Jurasinski, Gerald | |||
| 2 | DATE/TIME | Date/Time | Jurasinski, Gerald | Geocode | ||
| 3 | Station label | Station | Jurasinski, Gerald | |||
| 4 | Core | Core | Jurasinski, Gerald | |||
| 5 | Depth, description | Depth desc | Jurasinski, Gerald | cm | ||
| 6 | DEPTH, soil | Depth soil | m | Jurasinski, Gerald | Geocode – Anchor - Specific depth within the depth zone | |
| 7 | Comment | Comment | Jurasinski, Gerald | Pre-rewetting / Post-rewetting | ||
| 8 | Carbon dioxide, porewater | CO2 pw | µmol/l | Jurasinski, Gerald | Gas chromatography - thermal conductivity detection (GC-TCD), Agilent Technologies, 7890A | |
| 9 | Methane, porewater | CH4 pw | µmol/l | Jurasinski, Gerald | Gas chromatography - flame ionization detection (GC-FID), Agilent Technologies, 7890A | |
| 10 | δ13C, carbon dioxide | δ13C CO2 | ‰ PDB | Jurasinski, Gerald | Cavity ringdown spectrometer, Picarro, G2201-i | porewater |
| 11 | δ13C, methane | δ13C CH4 | ‰ PDB | Jurasinski, Gerald | Cavity ringdown spectrometer, Picarro, G2201-i | porewater |
| 12 | Gene copies per gram sediment | GCN per sed | #/g | Jurasinski, Gerald | Real-time quantitative polymerase chain reaction (qPCR) detection system, Bio-Rad, CFX Connect; primer: Eub341-F/Eub534-R (Muyzer et al., 1993) | X16S: 16S rRNA for bacteria |
| 13 | Gene copies per gram sediment | GCN per sed | #/g | Jurasinski, Gerald | Real-time quantitative polymerase chain reaction (qPCR) detection system, Bio-Rad, CFX Connect; primer: Eub341-F/Eub534-R (Muyzer et al., 1993) | mcrA: Methyl coenzyme M reductase α-subunit for methanogens |
| 14 | Gene copies per gram sediment | GCN per sed | #/g | Jurasinski, Gerald | Real-time quantitative polymerase chain reaction (qPCR) detection system, Bio-Rad, CFX Connect; primer: Eub341-F/Eub534-R (Muyzer et al., 1993) | pmoA: Particulate methane monooxygenase for methanotrophs |
| 15 | Gene copies per gram sediment | GCN per sed | #/g | Jurasinski, Gerald | Real-time quantitative polymerase chain reaction (qPCR) detection system, Bio-Rad, CFX Connect; primer: Eub341-F/Eub534-R (Muyzer et al., 1993) | dsrB: Dissimilatory sulfate reductase β-subunit for sulfate reducing bacteria |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1339 data points