Plum, Christoph; Gerhard, Miriam; Smykala, Mike (2024): Log response ratio of specific growth rates of Antarctic phytoplankton in monocultures versus mixed cultures in response to temperature and nitrogen:phosphorus supply interactions [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.971328 (DOI registration in progress), In: Plum, C et al. (2024): Growth rate, carrying capacity and stoichiometry of Antarctic phytoplankton in response to temperature and nitrogen:phosphorus supply interactions [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.971329
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Abstract:
Data were obtained from a laboratory growth experiment conducted from April to May 2021 at the Institute for Chemistry and Biology of the Marine Environment (University of Oldenburg). Three Antarctic phytoplankton species (Chaetoceros simplex, Phaeocystis antarctica (single cell culture) and Geminigera cryophila) were grown in monocultures and a mixture of all three species. We used a factorial design including four different N/P treatments (control plus three different N/P levels) and 10 different temperatures (-2, -1, 0, 1, 2, 3, 4, 6, 8 and 10°C). The detailed experimental setup is explained in doi:10.1594/PANGAEA.971329. Differences in growth rates of the individual species between monocultures and the assemblage were assessed by comparing their specific growth rates. Therefore, the biovolume of single species in the mixture was transformed to optical density (OD) based on biovolume-OD calibrations, and specific growth rates were calculated for each species in monoculture and the mixture as the regression between the start conditions and the exponential growth phase using the formula ln(Nt/N0)/t) were N0 is the OD at the start of the experiment and Nt the OD at the exponential growth phase of the mixture. In order to evaluate the effect size of the temperature and nutrient treatments on the differences in the specific growth rate of the individual species between monocultures and in the mixed assemblage, we calculated a log response ratio for each species and treatment as ln(µmix/µmono). Positive deviations from 0 indicate higher species-specific growth in the assemblage compared to the monoculture. Negative deviations from 0, in turn, indicate higher specific growth rates in monocultures.
Keyword(s):
Supplement to:
Smykala, Mike; Gerhard, Miriam; Plum, Christoph (submitted): Nutrient-dependent thermal response in growth and stoichiometry of Antarctic phytoplankton. Limnology and Oceanography
Funding:
German Research Foundation (DFG), grant/award no. 442927665: Altered stoichiometry and species interactions in coastal Antarctic plankton communities under global climate change
Comment:
The experiment started at 2021-04-02T10:00 and ended at 2021-05-19T12:00 .
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Plum, Christoph | |||
| 2 | Species, unique identification | Species UID | Plum, Christoph | |||
| 3 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Plum, Christoph | |||
| 4 | Species, unique identification (URI) | Species UID (URI) | Plum, Christoph | |||
| 5 | Culture | Culture | Plum, Christoph | |||
| 6 | Time in days | Time | days | Plum, Christoph | ||
| 7 | Treatment: temperature | T:temp | °C | Plum, Christoph | ||
| 8 | Treatment: Nitrogen/Phosphorus ratio | T:N/P | Plum, Christoph | molar N:P supply | ||
| 9 | Experimental treatment | Exp treat | Plum, Christoph | "P addition", "N addition", "N and P addition", Control | ||
| 10 | Treatment: nitrogen | T: N | µmol/l | Plum, Christoph | Nitrogen supply | |
| 11 | Treatment: phosphorus | T: P | µmol/l | Plum, Christoph | Phosphorus supply | |
| 12 | Replicate | Repl | Plum, Christoph | |||
| 13 | Abundance per volume | Abund v | #/ml | Plum, Christoph | Inverted microscope, Leica, DMIL | cells per ml |
| 14 | Biovolume | Biovol | µm3/ml | Plum, Christoph | Calculated | |
| 15 | Optical density at 440 nm | OD440 | Plum, Christoph | OD of monocultures was measured using a microplate reader (Biotek, USA) and OD in mixed cultures was calculated based on calibrations from cell numbers and biovolume. | ||
| 16 | Growth rate | µ | 1/day | Plum, Christoph | Calculated | specific growth rate, OD-based |
| 17 | Log response ratio, specific growth rate in mono versus mixed culture | LRR µ mono vs µ mix | Plum, Christoph | Calculated | LRR1 |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
14659 data points
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