Hildebrand, Jasper; Stumpp, Meike: Enzymatic analysis of carbohydrate metabolism in Strongylocentrotus purpuratus larvae during laboratory experiments with different temperature treatments [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.968990 (dataset in review), In: Hildebrand, J; Stumpp, M: Genetic and enzymatic analyses of carbohydrate metabolism in Strongylocentrotus purpuratus larvae [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.968977 (dataset in review)
Abstract:
Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2022 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu in the Christian-Albrechts-Universität zu Kiel at 10 °C. Carbohydrate-degrading enzymatic activity against various substrates (starch, laminarin, cellulose, xylan, trehalose) was assessed using 3,5-dinitrosalicylic acid (DNS, Miller, 1959). The activities of degradation were quantified by measuring the rate of reducing sugar production resulting from the hydrolysis of soluble starch, carboxymethylcellulose sodium salt (medium viscosity), laminarin from Laminaria digitata, xylan and D-(+)-Trehalose dihydrate under different pHs. For all reactions the amount of reducing sugar was determined spectrophotometrically at 540 nm with glucose as the standard (xylose for xylan characterization).
Keyword(s):
Supplement to:
Hildebrand, Jasper; Stumpp, Meike (submitted): Laminarin as important carbon source for larval invertebrates: Molecular analysis of carbohydrate metabolism in Strongylocentrotus purpuratus larvae. Journal of Experimental Biology
References:
Miller, Gail Lorenz (1959): Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry, 31(3), 426-428, https://doi.org/10.1021/ac60147a030
Funding:
German Research Foundation (DFG), grant/award no. 441084746: Black box larval physiology - mechanisms of nutrient acquisition and energetics of invertebrate larvae in a changing ocean
Coverage:
Latitude: 32.842674 * Longitude: -117.257767
Event(s):
Comment:
The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between September and November 2023. Larvae of cultured S. purpuratus were cultured for up to 10 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
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Negative values in the data tables are artifacts resulting from the calculation involving control and t₀ values in the photometric measurements.
Parameter(s):
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
612 data points