Hildebrand, Jasper; Stumpp, Meike: Genetic analysis of carbohydrate metabolism in Strongylocentrotus purpuratus larvae during laboratory experiments with different food treatments [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.968978 (dataset in review), In: Hildebrand, J; Stumpp, M: Genetic and enzymatic analyses of carbohydrate metabolism in Strongylocentrotus purpuratus larvae [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.968977 (dataset in review)
Abstract:
Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2022 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu in the Christian-Albrechts-Universität zu Kiel at 10 °C. For experimental characterization of carbohydrate digestion between September and November 2023 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu up to 10 days under two different food conditions. For genetic analysis of carbohydrate metabolism RT-qPCR was conducted. In brief, mRNA of samples from nine different time points (2 days post fertilization (dpf) – 10 dpf, n=4) was isolated and reverse transcripted into cDNA. Primer pairs were designed through NCBI primer blast, specifically targeting exon junctions of the selected carbohydrates enzyme genes for which the mRNA sequence is accessible in the S. purpuratus genome database echinobase.org. Genes were selected based on transcription patterns of the genes during early development. Primer specificity was validated through standard PCR, confirming the amplification of a single, specific product within the anticipated size range. Gene expression utilized the ∆∆CT method, and levels were normalized to the stable housekeeping gene EF1a (SPU_000595).
Keyword(s):
Supplement to:
Hildebrand, Jasper; Stumpp, Meike (submitted): Laminarin as important carbon source for larval invertebrates: Molecular analysis of carbohydrate metabolism in Strongylocentrotus purpuratus larvae. Journal of Experimental Biology
Funding:
German Research Foundation (DFG), grant/award no. 441084746: Black box larval physiology - mechanisms of nutrient acquisition and energetics of invertebrate larvae in a changing ocean
Coverage:
Latitude: 32.842674 * Longitude: -117.257767
Event(s):
Comment:
The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between September and November 2023. Larvae of cultured S. purpuratus were cultured for up to 10 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
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Experimental food treatments:
* Low food: 500 cells Rhodomonas sp. used / ml total culture
* High food: 8000 cells Rhodomonas sp. used / ml total culture
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Hildebrand, Jasper | ID | ||
| 2 | Sampling date/time, experiment | Date/time sampling exp | Hildebrand, Jasper | |||
| 3 | Sample ID | Sample ID | Hildebrand, Jasper | |||
| 4 | Type of study | Study type | Hildebrand, Jasper | |||
| 5 | Laboratory | Lab | Hildebrand, Jasper | |||
| 6 | Treatment: food | T:food | Hildebrand, Jasper | Microscope, Zeiss, Axio Imager Z; measured with Hemocytometer | ||
| 7 | Strongylocentrotus purpuratus, larval age | S. purpuratus larv age | days | Hildebrand, Jasper | Days post fertilization | |
| 8 | Strongylocentrotus purpuratus, larvae, alpha-amylase, relative gene expression | S. purpuratus larv a-amylase | Hildebrand, Jasper | Real-time-PCR-system, ThermoFisher Scientific, QuantStudio 1 | ||
| 9 | Strongylocentrotus purpuratus, larvae, beta-1,3-glucanase, relative gene expression | S. purpuratus larv b-1,3-glucanase | Hildebrand, Jasper | Real-time-PCR-system, ThermoFisher Scientific, QuantStudio 1 | ||
| 10 | Strongylocentrotus purpuratus, larvae, endo-beta-1,4-glucanase D-like, relative gene expression | S. purpuratus larv e-b-1,4-glucanase D-l | Hildebrand, Jasper | Real-time-PCR-system, ThermoFisher Scientific, QuantStudio 1 | ||
| 11 | Strongylocentrotus purpuratus, larvae, xylanase/beta-glucanase-like, relative gene expression | S. purpuratus larv xylanase/b-glucanase- | Hildebrand, Jasper | Real-time-PCR-system, ThermoFisher Scientific, QuantStudio 1 | ||
| 12 | Strongylocentrotus purpuratus, larvae, trehalase, relative gene expression | S. purpuratus larv trehalase | Hildebrand, Jasper | Real-time-PCR-system, ThermoFisher Scientific, QuantStudio 1 |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
864 data points