Citation:

PANGAEA.968627

Name: Kelp holobiont responses to run-off in Billefjorden, Svalbard in August 2022
Published: 2024-12-19
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Biochemistry; heavy metals; Holobiont; microbial community; Rare earth elements; Saccharina latissima; seaweed

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Published: 2024-12-19 • DOI registered: 2025-01-17

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Abstract:

Kelps act as ecosystem engineers and foundation species, providing the food web basis for Arctic coastal, rocky shore ecosystems. Arctic fjords are undergoing large climatic changes, with the consequences of glacial and terrestrial run-off on kelp holobionts largely being unknown. We compared areas being influenced by either glacial or terrestrial run-off with a control area in Billefjorden, Svalbard in August 2022. Kelps were collected from three sampling stations (A-I) per area, with a plant rake (Plant rake 19.000, acc. to Sigurd Olsen, KC Denmark, Silkeborg, Denmark) on 5±2 m water depth. Silica-dried meristematic tissue was used for biochemical (pigment, antioxidant activity) analyses. The tissue above the meristem was rinsed with ultrapure water and freeze-dried, before elements were measured of homogenized, digested material with a triple quadrupole ICP-MS/MS system (Agilent 8800, Agilent Technologies; Tokyo, Japan). For microbial analyses, an area of 10×10 cm above the meristem was swabbed with a sterile cotton swab. The V4-V5 regions (515F: 5'-GTGCCAGCMGCCGCGGTAA-3' and 907R: 5'-CCGTCAATTCMTTTRAGTTT-3') of the bacterial 16S rRNA gene from DNA extracts were amplified and sequenced using the Illumina Nova platform (Shanghai Hanyu Biotech lab, Shanghai, China).

Keyword(s):

Biochemistry; heavy metals; Holobiont; microbial community; Rare earth elements; Saccharina latissima; seaweed

Related to:

Niedzwiedz, Sarina; Schmidt, Claudia Elena; Yang, Yunlan; Burgunter-Delamare, Bertille; Andersen, Sebastian; Hildebrandt, Lars; Pröfrock, Daniel; Thomas, Helmuth; Zhang, Rui; Damsgård, Børge; Bischof, Kai (2024): Run-off impacts on Arctic kelp holobionts have strong implications on ecosystem functioning and bioeconomy. Scientific Reports, 14(1), 30506, https://doi.org/10.1038/s41598-024-82287-w

Project(s):

Arctic Biodiversity & Livelihoods (FACE-IT)

Funding:

Horizon 2020 (H2020), grant/award no. 869383 : Arctic biodiversity change and its consequences: Assessing, monitoring and predicting the effects of ecosystem tipping cascades on marine ecosystem services and dependent human systems (ECOTIP)

National Key Research and Development Program of China, grant/award no. 2021YFE0193000

Southern Marine Science and Engineering Guangdong Laboratory, grant/award no. SML2023SP218

Coverage:

Median Latitude: 78.647108 * Median Longitude: 16.624513 * South-bound Latitude: 78.601361 * West-bound Longitude: 16.398833 * North-bound Latitude: 78.691111 * East-bound Longitude: 16.902333

Date/Time Start: 2022-08-22T00:00:00 * Date/Time End: 2022-08-29T00:00:00

Event(s):

Billefjorden * Latitude: 78.487000 * Longitude: 16.197000 * Location: Svalbard * Method/Device: Biological sample (BIOS)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Sample ID	Sample ID		Niedzwiedz, Sarina
2	Sample position	Samp pos		Niedzwiedz, Sarina		Sampling area
3	Station label	Station		Niedzwiedz, Sarina
4	Replicates	Repl	#	Niedzwiedz, Sarina
5	DATE/TIME	Date/Time		Niedzwiedz, Sarina		Geocode
6	LATITUDE	Latitude		Niedzwiedz, Sarina		Geocode
7	LONGITUDE	Longitude		Niedzwiedz, Sarina		Geocode
8	Chlorophyll c2, per dry mass	Chl c2/dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
9	Fucoxanthin, per dry mass	Fuco dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
10	Violaxanthin, per dry mass	Viola/dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
11	Antheraxanthin, per dry mass	Anthera/dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
12	Zeaxanthin, per dry mass	Zea/dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
13	Chlorophyll a, per dry mass	Chl a/dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
14	beta-Carotene, per dry mass	b-Car/dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
15	Violaxanthin + Antheraxanthin + Zeaxanthin, per dry mass	VAZ/dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
16	Xanthophyll cycle pigments/chlorophyll a	VAZ/chl a		Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
17	De-expoxidation state of xanthophyll cycle	DPS		Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
18	Pigments, accessory, per dry mass	Pigm acc/dm	µg/g	Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
19	Pigments, accessory/chlorophyll a ratio	Pigm acc/chl a		Niedzwiedz, Sarina	High Performance Liquid Chromatography (HPLC)
20	Antioxidant capacity, in Trolox Equivalents, per dry mass	TEAC/dm	mmol/g	Niedzwiedz, Sarina	According to the ABTS method
21	Calcium	Ca	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
22	Cadmium	Cd	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
23	2,6,11,15-tetramethylhexadecane	Crocetane	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
24	Copper	Cu	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
25	Iron	Fe	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
26	Mercury	Hg	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
27	Potassium	K	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
28	Magnesium	Mg	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
29	Manganese	Mn	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
30	Molybdenum	Mo	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
31	Sodium	Na	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
32	Lead	Pb	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
33	Zinc	Zn	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
34	Aluminium	Al	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
35	Barium	Ba	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
36	Bismuth	Bi	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
37	Beryllium	Be	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
38	Cerium	Ce	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
39	Cobalt	Co	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
40	Dysprosium	Dy	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
41	Erbium	Er	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
42	Europium	Eu	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
43	Gadolinium	Gd	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
44	Holmium	Ho	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
45	Lanthanum	La	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
46	Lithium	Li	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
47	Lutetium	Lu	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
48	Neodymium	Nd	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
49	Nickel	Ni	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
50	Phosphorus	P	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
51	Palladium	Pd	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
52	Praseodymium	Pr	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
53	Rubidium	Rb	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
54	Ruthenium	Ru	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
55	Antimony	Sb	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
56	Samarium	Sm	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
57	Tin	Sn	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
58	Strontium	Sr	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
59	Scandium	Sc	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
60	Terbium	Tb	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
61	Thorium	Th	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
62	Titanium	Ti	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
63	Thulium	Tm	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
64	Uranium	U	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
65	Vanadium	V	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
66	Ytterbium	Yb	µg/g	Niedzwiedz, Sarina	Tandem Mass Spectrometry (ICP-MS/MS)
67	Amplicon sequence variant	ASV		Niedzwiedz, Sarina	Calculated	Observed features
68	Pielou evenness index	J'		Niedzwiedz, Sarina	Calculated
69	Phylogenetic diversity	P div		Niedzwiedz, Sarina	Calculated
70	Bacteroidetes	Bacteroidetes	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
71	Planctomycetes	Planctomycetes	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
72	Proteobacteria	Proteobacteria	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
73	Alphaproteobacteria	Alphaproteobacteria	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
74	Flavobacteria	Flavobacteria	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
75	Gammaproteobacteria	Gammaproteobacteria	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
76	Planctomycetia	Planctomycetia	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
77	Saprospiraceae	Saprospiraceae	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
78	Bacteria, unclassified	Bac unclassif	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]	Order
79	Flavobacteriales	Flavobacteriales	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
80	Pirellulales	Pirellulales	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
81	Rhodobacteraceae	Rhodobacteraceae	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
82	Saprospirales	Saprospirales	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
83	Thiotrichales	Thiotrichales	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
84	Bacteria, unclassified	Bac unclassif	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]	Family
85	Flavobacteriaceae	Flavobacteriaceae	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
86	Pirellulaceae	Pirellulaceae	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
87	Rhodobacteraceae	Rhodobacteraceae	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
88	Saprospiraceae	Saprospiraceae	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
89	Thiotrichaceae	Thiotrichaceae	%	Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]
90	Shannon Diversity Index	H'		Niedzwiedz, Sarina	16S rRNA gene sequencing [V4-V5 variable regions]

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

6005 data points

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