Möller, Kristof; Tillmann, Urban; Pöchhacker, Magdalena; Varga, Elisabeth; Krock, Bernd; Koch, Florian; Harris, Thomas M; Meunier, Cédric Léo: Bioassays quantifying the adverse effects of three strains of the harmful dinoflagellate Alexandrium pseudogonyaulax on the viability of the RTgill-W1 cell line of the rainbow trout (Oncorhynchus mykiss) [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.968616 (dataset in review), In: Möller, K et al.: Bioassays quantifying the adverse effects of three strains of the harmful dinoflagellate Alexandrium pseudogonyaulax and goniodomins (GDs) on the viability and membrane integrity of the RTgill-W1 cell line of the rainbow trout (Oncorhynchus mykiss) [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.968675 (dataset in review)
Abstract:
RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) were subjected to supernatants from exponentially grown Alexandrium pseudogonyaulax cultures for 24 h to create dose-response curves. The bioassays were carried out with 300 µl of total incubation volume in 96-well plates. After 24 hours, residual supernatants were thoroughly siphoned off and the metabolic activity of the remaining gill cells was assessed using a cell viability assay kit (CellTiter-Blue®, Promega, G8080) following the standard protocol provided by the manufacturer. The bioassays were analysed fluorometrically using a cell-imaging multi-mode reader (Cytation™ 3 Cell Imaging Multi-Mode Reader, BioTek). All data was pre-filtered according to (1) visual inspection of the gill cell densities in the 96-well plates and if densities differed greatly data was excluded, and (2) data were grouped by each biological gill cell line replicate, each biological A. pseudogonyaulax replicate, each strain and each cell density equivalent and subjected to a Dixon outlier test. If the Dixon test was significant, data was excluded. This dataset contains four to six biological gill cell line replicates tested with three different A. pseudogonyaulax strains, each comprised up to three biological replicates. Each biological replicate of each A. pseudogonyaulax strain was tested with a total of two to six cell density equivalents of cell-free supernatants, each comprised of two to six technical replicates. The bioassays were carried out between May 19 and October 10, 2023 at the Department of Food Chemistry and Toxicology (LMC), Faculty of Chemistry, University of Vienna.
Keyword(s):
Supplement to:
Möller, Kristof; Tillmann, Urban; Pöchhacker, Magdalena; Varga, Elisabeth; Krock, Bernd; Koch, Florian; Harris, Thomas M; Meunier, Cédric Léo (in prep.): Toxic effects of the emerging Alexandrium pseudogonyaulax (Dinophyceae) on four trophic levels of the pelagic food web. Harmful Algae
Funding:
Helmholtz Association of German Research Centres (HGF), grant/award no. Changing-Earth_Subtopic_6.2: Adaptation of marine life: From genes to ecosystems
Coverage:
Latitude: 56.630000 * Longitude: 8.290000
Date/Time Start: 2020-08-17T09:10:00 * Date/Time End: 2020-08-17T09:10:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Möller, Kristof | Bioassay | ||
| 2 | Date/time start, experiment | Date/time start exp | Möller, Kristof | |||
| 3 | Date/time end, experiment | Date/time end exp | Möller, Kristof | |||
| 4 | Incubation duration | Inc dur | h | Möller, Kristof | ||
| 5 | Incubation volume | Inc vol | µl | Möller, Kristof | Microwell plate incubation | 96-well plate |
| 6 | Treatment: toxin | T: toxin | Möller, Kristof | Medium used | ||
| 7 | Species, unique identification | Species UID | Lytic species | |||
| 8 | Species, unique identification (URI) | Species UID (URI) | Lytic species | |||
| 9 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Lytic species | |||
| 10 | Alexandrium pseudogonyaulax, strain | A. pseudogonyaulax strain | Möller, Kristof | |||
| 11 | Replicate, biological | Repl bio | Möller, Kristof | Biological Alexandrium pseudogonyaulax replicate | ||
| 12 | Alexandrium, cell density, equivalent | Alexandrium cells eq | #/ml | Möller, Kristof | Alexandrium cells centrifuged off, supernatant diluted with K-medium to obtain different concentrations | Visual identification (VID) of Lugol preserved samples |
| 13 | Replicate, technical | Repl tech | Möller, Kristof | |||
| 14 | Species, unique identification | Species UID | RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) | |||
| 15 | Species, unique identification (URI) | Species UID (URI) | RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) | |||
| 16 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) | |||
| 17 | Replicate, biological | Repl bio | Möller, Kristof | Biological RTgill-W1 gill cell replicate; gill cell densities in the 96-well plates were visually inspected and data were excluded if densities differed greatly | ||
| 18 | Viability, gill cells of Oncorhynchus mykiss, relative | Viab gill cells O. mykiss rel | % | Möller, Kristof | CellTiter-Blue® Cell Viability Assay kit, Promega, G8080; measured with cell imaging multi-mode reader, BioTek, Cytation™ 3 | Gill cell viability (as percentage of control) was assessed using the CellTiter-Blue® cell viability assay kit (Promega, G8080) following the standard protocol provided by the manufacturer; assay results were assessed fluorometrically using a cell-imaging multi-mode reader (Cytation™ 3 Cell Imaging Multi-Mode Reader, BioTek). |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
9184 data points
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