Vermandele, Fanny; Sasaki, Matthew; Winkler, Gesche; Dam, Hans G; Madeira, Diana; Calosi, Piero: Sex-specific physiological responses and traits (metabolic rates, upper thermal limit, length, and dry weight) of the marine calanoid copepod Acartia tonsa under combined exposure to hypoxia and marine heatwave events [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.967350 (dataset in review), In: Vermandele, F et al.: Survival, life-history, and physiological responses of the marine calanoid copepod Acartia tonsa under combined hypoxia and marine heatwave events [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.967352 (dataset in review)
Abstract:
Specimens of the marine calanoid copepod Acartia tonsa were exposed for five days under laboratory conditions to the isolated or combined effects of hypoxia and a marine heatwave event to test for their sex-specific life-history and physiological responses to these stressors. Four treatments were used: control (C: 18 °C, 100 % O₂ sat.), hypoxia (H: 18 °C, 35 % O₂ sat.), marine heatwave (MHW: 25 °C, 100 % O₂ sat.) and combined conditions (HMHW: 25 °C, 35 % O₂ sat.). This dataset compiles all morphological and physiological measurements, namely routine metabolic oxygen consumption rates (µmol h⁻¹ and µmol mg⁻¹ h⁻¹), upper thermal limit (°C) as well as length (mm) and dry weight (µg) following the five-day laboratory exposure experiment.
Keyword(s):
Supplement to:
Vermandele, Fanny; Sasaki, Matthew; Winkler, Gesche; Dam, Hans G; Madeira, Diana; Calosi, Piero (in review): When the going gets tough, the females get going: sex-specific physiological responses to simultaneous exposure to hypoxia and marine heatwave events. Global Change Biology
References:
Dam, Hans G; deMayo, James A; Park, Gihong; Norton, Lydia; He, Xuejia; Finiguerra, Michael B; Baumann, Hannes; Brennan, Reid S; Pespeni, Melissa H (2021): Rapid, but limited, zooplankton adaptation to simultaneous warming and acidification. Nature Climate Change, 11(9), 780-786, https://doi.org/10.1038/s41558-021-01131-5
Harada, Alice E; Healy, Timothy M; Burton, Ronald S (2019): Variation in Thermal Tolerance and Its Relationship to Mitochondrial Function Across Populations of Tigriopus californicus. Frontiers in Physiology, 10, 213, https://doi.org/10.3389/fphys.2019.00213
Healy, Timothy M; Bock, Antonia K; Burton, Ronald S (2019): Variation in developmental temperature alters adulthood plasticity of thermal tolerance in Tigriopus californicus. Journal of Experimental Biology, 222 (22), jeb.213405, https://doi.org/10.1242/jeb.213405
Kiørboe, Thomas; Møhlenberg, Flemming; Hamburger, Kirsten (1985): Bioenergetics of the planktonic copepod Acartia tonsa: relation between feeding, egg production and respiration, and composition of specific dynamic action. Marine Ecology Progress Series, 26, 85-97, https://doi.org/10.3354/meps026085
Funding:
Natural Sciences and Engineering Research Council (NSERC), grant/award no. RGPIN-2020-05627: Research Support for New Academics (FRQNT)
Coverage:
Latitude: 41.320725 * Longitude: -72.001643
Event(s):
Comment:
The experiment was conducted in the Marine Ecological and Evolutionary Physiology Laboratory (MEEP) at the University of Quebec in Rimouski (UQAR), Rimouski, QC, Canada. Copepods were maintained following the stock culture culturing protocol described in Dam et al. 2021 (https://doi.org/10.1038/s41558-021-01131-5). Namely, approximately 200 copepods were kept in three 5 L aquaria filled with artificial seawater kept at a salinity of 28.4 ± 0.5. The physicochemical parameters of the seawater were the following: temperature of 18 ± 0.4 °C, oxygen saturation of 93.7 ± 6.0 %, pH NBS of 8.20 ± 0.09. The photoperiod was kept at 13h light: 11 h dark. Copepods were fed ad libitum with a mixture of three phytoplankters: Tetraselmis sp., Thalassiosira weissflogii and Rhodomonas salina.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Vermandele, Fanny | |||
| 2 | Sampling date/time, experiment | Date/time sampling exp | Vermandele, Fanny | |||
| 3 | Treatment: temperature | T:temp | °C | Vermandele, Fanny | ||
| 4 | Treatment: Oxygen saturation | T:O2 sat | % | Vermandele, Fanny | ||
| 5 | Treatment | Treat | Vermandele, Fanny | C: Control treatment (18 °C, 100 % O2 sat.) / H: Hypoxia treatment (18 °C, 35 % O2 sat.) / MHW: Marine heatwave treatment (25 °C, 100 % O2 sat.) / HMHW: Combined treatment (25 °C, 35 % O2 sat.) | ||
| 6 | Number | No | Vermandele, Fanny | System No, refers to the number ID attributed to each shelf holding four aquaria each | ||
| 7 | Aquaria ID | Aquaria ID | Vermandele, Fanny | |||
| 8 | Acartia tonsa, sex | A. tonsa sex | Vermandele, Fanny | F= Females M= Males | ||
| 9 | Acartia tonsa, routine metabolic oxygen consumption | A. tonsa MO2 | µmol/h | Vermandele, Fanny | Respirometry plate, PreSens, SDR SensorDish | Routine metabolic rate per individual |
| 10 | Acartia tonsa, routine metabolic oxygen consumption, specific | A. tonsa spec MO2 | µmol/mg/h | Vermandele, Fanny | Respirometry plate, PreSens, SDR SensorDish | Individual routine metabolic rate divided by individual weight |
| 11 | Acartia tonsa, critical thermal maximum | A. tonsa CTmax | °C | Vermandele, Fanny | CTmax method, modified protocol using the definition of Loss of Locomotor Performance used as endpoint by Harada et al. (2019) and Healy et al. (2019) | |
| 12 | Acartia tonsa, prosome length | A. tonsa prosome l | mm | Vermandele, Fanny | Stereomicroscope, Leica; coupled with Integrated CMOS microscope camera, Leica, IC90 E | Length was measured on pictures using ImageJ |
| 13 | Acartia tonsa, dry mass | A. tonsa dm | µg | Vermandele, Fanny | Calculated according to Kiørboe et al. (1985) | Equation was used to convert prosome lenght to dry weight |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1378 data points