Leys, Sally P; Federwisch, Luisa (2024): Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.966335, In: Federwisch, Luisa; Leys, Sally P; Janussen, Dorte; Linse, Katrin; Hentschel, Ute; Busch, Kathrin (2024): Images and microbiome data of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected during expeditions PS96 and JR17003a in the Weddell Sea, Antarctica [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.965868
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Abstract:
This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale.
Keyword(s):
Project(s):
Bentho-Pelagic Processes @ AWI (AWI_BPP)
Coverage:
Median Latitude: -63.826788 * Median Longitude: -58.016557 * South-bound Latitude: -64.057780 * West-bound Longitude: -58.477560 * North-bound Latitude: -63.615860 * East-bound Longitude: -57.501430
Date/Time Start: 2018-03-05T14:18:00 * Date/Time End: 2018-03-07T17:54:00
Minimum Elevation: -845.0 m * Maximum Elevation: -429.0 m
Event(s):
JR17003A_38 * Latitude Start: -64.053780 * Longitude Start: -58.476010 * Latitude End: -64.057780 * Longitude End: -58.477560 * Date/Time: 2018-03-05T14:18:00 * Elevation Start: -787.0 m * Elevation End: -740.0 m * Location: Prince Gustav Channel * Campaign: JR17003A * Basis: James Clark Ross * Method/Device: Agassiz Trawl (AGT)
JR17003A_46 * Latitude Start: -63.806030 * Longitude Start: -58.065230 * Latitude End: -63.810580 * Longitude End: -58.070640 * Date/Time: 2018-03-06T17:48:00 * Elevation Start: -845.0 m * Elevation End: -825.0 m * Location: Prince Gustav Channel * Campaign: JR17003A * Basis: James Clark Ross * Method/Device: Agassiz Trawl (AGT)
JR17003A_52 * Latitude Start: -63.615860 * Longitude Start: -57.501430 * Latitude End: -63.616700 * Longitude End: -57.508470 * Date/Time: 2018-03-07T17:54:00 * Elevation Start: -489.0 m * Elevation End: -429.0 m * Location: Prince Gustav Channel * Campaign: JR17003A * Basis: James Clark Ross * Method/Device: Agassiz Trawl (AGT)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Federwisch, Luisa | |||
| 2 | Latitude of event | Latitude | Federwisch, Luisa | |||
| 3 | Longitude of event | Longitude | Federwisch, Luisa | |||
| 4 | Elevation of event | Elevation | m | Federwisch, Luisa | ||
| 5 | Sample ID | Sample ID | Federwisch, Luisa | |||
| 6 | Image number/name | Image no/name | Federwisch, Luisa | |||
| 7 | Image | IMAGE | Federwisch, Luisa | Transmission electron microscope (TEM) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Basic curation (CurationLevelB)
Size:
360 data points