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Leys, Sally P; Federwisch, Luisa (2024): Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.966335, In: Federwisch, Luisa; Leys, Sally P; Janussen, Dorte; Linse, Katrin; Hentschel, Ute; Busch, Kathrin (2024): Images and microbiome data of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected during expeditions PS96 and JR17003a in the Weddell Sea, Antarctica [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.965868

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Abstract:
This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale.
Keyword(s):
Antarctic; Demospongiae; Hexactinellida; JR17003a; microbes; microbiome; microscopy; Sponges; TEM; transmission electron microscopy; ultrastructure; Weddell Sea
Project(s):
Coverage:
Median Latitude: -63.826788 * Median Longitude: -58.016557 * South-bound Latitude: -64.057780 * West-bound Longitude: -58.477560 * North-bound Latitude: -63.615860 * East-bound Longitude: -57.501430
Date/Time Start: 2018-03-05T14:18:00 * Date/Time End: 2018-03-07T17:54:00
Minimum Elevation: -845.0 m * Maximum Elevation: -429.0 m
Event(s):
JR17003A_38  * Latitude Start: -64.053780 * Longitude Start: -58.476010 * Latitude End: -64.057780 * Longitude End: -58.477560 * Date/Time: 2018-03-05T14:18:00 * Elevation Start: -787.0 m * Elevation End: -740.0 m * Location: Prince Gustav Channel * Campaign: JR17003A * Basis: James Clark Ross * Method/Device: Agassiz Trawl (AGT)
JR17003A_46  * Latitude Start: -63.806030 * Longitude Start: -58.065230 * Latitude End: -63.810580 * Longitude End: -58.070640 * Date/Time: 2018-03-06T17:48:00 * Elevation Start: -845.0 m * Elevation End: -825.0 m * Location: Prince Gustav Channel * Campaign: JR17003A * Basis: James Clark Ross * Method/Device: Agassiz Trawl (AGT)
JR17003A_52  * Latitude Start: -63.615860 * Longitude Start: -57.501430 * Latitude End: -63.616700 * Longitude End: -57.508470 * Date/Time: 2018-03-07T17:54:00 * Elevation Start: -489.0 m * Elevation End: -429.0 m * Location: Prince Gustav Channel * Campaign: JR17003A * Basis: James Clark Ross * Method/Device: Agassiz Trawl (AGT)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Event labelEventFederwisch, Luisa
Latitude of eventLatitudeFederwisch, Luisa
Longitude of eventLongitudeFederwisch, Luisa
Elevation of eventElevationmFederwisch, Luisa
Sample IDSample IDFederwisch, Luisa
Image number/nameImage no/nameFederwisch, Luisa
ImageIMAGEFederwisch, LuisaTransmission electron microscope (TEM)
Status:
Curation Level: Basic curation (CurationLevelB)
Size:
360 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

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Event

Latitude

Longitude

Elevation [m]

Sample ID

Image no/name

IMAGE
JR17003A_38 -64.0538-58.4760-78710401JR17003a_38_ID104_01.tif
JR17003A_38-64.0538-58.4760-78710402JR17003a_38_ID104_02.tif
JR17003A_38-64.0538-58.4760-78710403JR17003a_38_ID104_03.tif
JR17003A_38-64.0538-58.4760-78710404JR17003a_38_ID104_04.tif
JR17003A_38-64.0538-58.4760-78710405JR17003a_38_ID104_05.tif
JR17003A_38-64.0538-58.4760-78710406JR17003a_38_ID104_06.tif
JR17003A_38-64.0538-58.4760-78710407JR17003a_38_ID104_07.tif
JR17003A_38-64.0538-58.4760-78710408JR17003a_38_ID104_08.tif
JR17003A_46 -63.8060-58.0652-84528201JR17003a_46_ID282_01.tif
JR17003A_46-63.8060-58.0652-84528202JR17003a_46_ID282_02.tif
JR17003A_46-63.8060-58.0652-84528203JR17003a_46_ID282_03.tif
JR17003A_46-63.8060-58.0652-84528204JR17003a_46_ID282_04.tif
JR17003A_46-63.8060-58.0652-84528205JR17003a_46_ID282_05.tif
JR17003A_46-63.8060-58.0652-84528206JR17003a_46_ID282_06.tif
JR17003A_46-63.8060-58.0652-84528207JR17003a_46_ID282_07.tif
JR17003A_46-63.8060-58.0652-84528208JR17003a_46_ID282_08.tif
JR17003A_46-63.8060-58.0652-84528209JR17003a_46_ID282_09.tif
JR17003A_46-63.8060-58.0652-84528210JR17003a_46_ID282_10.tif
JR17003A_46-63.8060-58.0652-84528211JR17003a_46_ID282_11.tif
JR17003A_46-63.8060-58.0652-84528212JR17003a_46_ID282_12.tif
JR17003A_46-63.8060-58.0652-84528213JR17003a_46_ID282_13.tif
JR17003A_46-63.8060-58.0652-84528214JR17003a_46_ID282_14.tif
JR17003A_46-63.8060-58.0652-84528215JR17003a_46_ID282_15.tif
JR17003A_46-63.8060-58.0652-84528216JR17003a_46_ID282_16.tif
JR17003A_46-63.8060-58.0652-84528217JR17003a_46_ID282_17-bl2.tif
JR17003A_46-63.8060-58.0652-84528218JR17003a_46_ID282_18-bl2.tif
JR17003A_46-63.8060-58.0652-84528219JR17003a_46_ID282_19-bl2.tif
JR17003A_46-63.8060-58.0652-84528220JR17003a_46_ID282_20-bl2.tif
JR17003A_46-63.8060-58.0652-84528221JR17003a_46_ID282_21-bl2.tif
JR17003A_46-63.8060-58.0652-84528222JR17003a_46_ID282_22-bl2.tif
JR17003A_46-63.8060-58.0652-84528223JR17003a_46_ID282_23-bl2.tif
JR17003A_46-63.8060-58.0652-84528224JR17003a_46_ID282_24-bl2.tif
JR17003A_46-63.8060-58.0652-84528225JR17003a_46_ID282_25-bl2.tif
JR17003A_46-63.8060-58.0652-84528301JR17003a_46_ID283_01.tif
JR17003A_46-63.8060-58.0652-84528302JR17003a_46_ID283_02.tif
JR17003A_46-63.8060-58.0652-84528303JR17003a_46_ID283_03.tif
JR17003A_46-63.8060-58.0652-84528304JR17003a_46_ID283_04.tif
JR17003A_46-63.8060-58.0652-84528305JR17003a_46_ID283_05.tif
JR17003A_46-63.8060-58.0652-84528306JR17003a_46_ID283_06.tif
JR17003A_46-63.8060-58.0652-84528307JR17003a_46_ID283_07.tif
JR17003A_46-63.8060-58.0652-84528308JR17003a_46_ID283_08.tif
JR17003A_46-63.8060-58.0652-84528309JR17003a_46_ID283_09.tif
JR17003A_46-63.8060-58.0652-84528310JR17003a_46_ID283_10.tif
JR17003A_46-63.8060-58.0652-84528311JR17003a_46_ID283_11.tif
JR17003A_46-63.8060-58.0652-84530301JR17003a_46_ID303_01.tif
JR17003A_46-63.8060-58.0652-84530302JR17003a_46_ID303_02.tif
JR17003A_46-63.8060-58.0652-84530303JR17003a_46_ID303_03.tif
JR17003A_46-63.8060-58.0652-84530304JR17003a_46_ID303_04.tif
JR17003A_46-63.8060-58.0652-84530305JR17003a_46_ID303_05.tif
JR17003A_46-63.8060-58.0652-84530306JR17003a_46_ID303_06.tif
JR17003A_46-63.8060-58.0652-84530307JR17003a_46_ID303_07.tif
JR17003A_46-63.8060-58.0652-84530308JR17003a_46_ID303_08.tif
JR17003A_46-63.8060-58.0652-84530309JR17003a_46_ID303_09.tif
JR17003A_46-63.8060-58.0652-84530310JR17003a_46_ID303_10.tif
JR17003A_46-63.8060-58.0652-84530311JR17003a_46_ID303_11.tif
JR17003A_46-63.8060-58.0652-84530312JR17003a_46_ID303_12.tif
JR17003A_46-63.8060-58.0652-84530313JR17003a_46_ID303_13.tif
JR17003A_46-63.8060-58.0652-84530401JR17003a_46_ID304_01.tif
JR17003A_46-63.8060-58.0652-84530402JR17003a_46_ID304_02.tif
JR17003A_46-63.8060-58.0652-84530403JR17003a_46_ID304_03.tif
JR17003A_46-63.8060-58.0652-84530404JR17003a_46_ID304_04.tif
JR17003A_46-63.8060-58.0652-84530405JR17003a_46_ID304_05.tif
JR17003A_46-63.8060-58.0652-84530406JR17003a_46_ID304_06.tif
JR17003A_46-63.8060-58.0652-84530407JR17003a_46_ID304_07.tif
JR17003A_46-63.8060-58.0652-84530408JR17003a_46_ID304_08.tif
JR17003A_46-63.8060-58.0652-84530409JR17003a_46_ID304_09.tif
JR17003A_46-63.8060-58.0652-84530410JR17003a_46_ID304_10.tif
JR17003A_46-63.8060-58.0652-84530411JR17003a_46_ID304_11.tif
JR17003A_46-63.8060-58.0652-84530412JR17003a_46_ID304_12.tif
JR17003A_46-63.8060-58.0652-84530413JR17003a_46_ID304_13.tif
JR17003A_46-63.8060-58.0652-84530414JR17003a_46_ID304_14.tif
JR17003A_46-63.8060-58.0652-84530415JR17003a_46_ID304_15.tif
JR17003A_46-63.8060-58.0652-84530416JR17003a_46_ID304_16.tif
JR17003A_46-63.8060-58.0652-84530417JR17003a_46_ID304_17.tif
JR17003A_46-63.8060-58.0652-84530418JR17003a_46_ID304_18.tif
JR17003A_46-63.8060-58.0652-84535001JR17003a_46_ID350_01.tif
JR17003A_46-63.8060-58.0652-84535002JR17003a_46_ID350_02.tif
JR17003A_46-63.8060-58.0652-84535301JR17003a_46_ID353_01.tif
JR17003A_46-63.8060-58.0652-84535302JR17003a_46_ID353_02.tif
JR17003A_46-63.8060-58.0652-84535303JR17003a_46_ID353_03.tif
JR17003A_46-63.8060-58.0652-84535304JR17003a_46_ID353_04.tif
JR17003A_46-63.8060-58.0652-84535305JR17003a_46_ID353_05.tif
JR17003A_46-63.8060-58.0652-84535306JR17003a_46_ID353_06.tif
JR17003A_46-63.8060-58.0652-84535307JR17003a_46_ID353_07.tif
JR17003A_46-63.8060-58.0652-84535308JR17003a_46_ID353_08.tif
JR17003A_46-63.8060-58.0652-84535309JR17003a_46_ID353_09.tif
JR17003A_46-63.8060-58.0652-84535310JR17003a_46_ID353_10.tif
JR17003A_46-63.8060-58.0652-84535311JR17003a_46_ID353_11.tif
JR17003A_46-63.8060-58.0652-84535312JR17003a_46_ID353_12.tif
JR17003A_46-63.8060-58.0652-84535313JR17003a_46_ID353_13.tif
JR17003A_46-63.8060-58.0652-84535314JR17003a_46_ID353_14.tif
JR17003A_46-63.8060-58.0652-84535315JR17003a_46_ID353_15.tif
JR17003A_46-63.8060-58.0652-84535316JR17003a_46_ID353_16.tif
JR17003A_46-63.8060-58.0652-84535317JR17003a_46_ID353_17.tif
JR17003A_46-63.8060-58.0652-84535318JR17003a_46_ID353_18.tif
JR17003A_46-63.8060-58.0652-84535319JR17003a_46_ID353_19.tif
JR17003A_46-63.8060-58.0652-84535320JR17003a_46_ID353_20.tif
JR17003A_46-63.8060-58.0652-84535321JR17003a_46_ID353_21.tif
JR17003A_46-63.8060-58.0652-84535322JR17003a_46_ID353_22.tif
JR17003A_46-63.8060-58.0652-84535323JR17003a_46_ID353_23.tif
JR17003A_46-63.8060-58.0652-84535324JR17003a_46_ID353_24.tif
JR17003A_46-63.8060-58.0652-84535325JR17003a_46_ID353_25.tif
JR17003A_46-63.8060-58.0652-84535326JR17003a_46_ID353_26.tif
JR17003A_46-63.8060-58.0652-84535327JR17003a_46_ID353_27.tif
JR17003A_46-63.8060-58.0652-84535328JR17003a_46_ID353_28.tif
JR17003A_46-63.8060-58.0652-84535329JR17003a_46_ID353_29.tif
JR17003A_46-63.8060-58.0652-84535330JR17003a_46_ID353_30.tif
JR17003A_46-63.8060-58.0652-84535331JR17003a_46_ID353_31.tif
JR17003A_52 -63.6159-57.5014-48939801JR17003a_52_ID398_01.tif
JR17003A_52-63.6159-57.5014-48939802JR17003a_52_ID398_02.tif
JR17003A_52-63.6159-57.5014-48939803JR17003a_52_ID398_03.tif
JR17003A_52-63.6159-57.5014-48939804JR17003a_52_ID398_04.tif
JR17003A_52-63.6159-57.5014-48939805JR17003a_52_ID398_05.tif
JR17003A_52-63.6159-57.5014-48939806JR17003a_52_ID398_06.tif
JR17003A_52-63.6159-57.5014-48939807JR17003a_52_ID398_07.tif
JR17003A_52-63.6159-57.5014-48939808JR17003a_52_ID398_08.tif
JR17003A_52-63.6159-57.5014-48939809JR17003a_52_ID398_09.tif
JR17003A_52-63.6159-57.5014-48939810JR17003a_52_ID398_10.tif
JR17003A_52-63.6159-57.5014-48939811JR17003a_52_ID398_11.tif
JR17003A_52-63.6159-57.5014-48939812JR17003a_52_ID398_12.tif