Citation:

PANGAEA.966001

Name: Digestion rate in larval Strongylocentrotus purpuratus during laboratory experiments with low protease inhibitor treatments
Published: 2024-04-18
License: https://creativecommons.org/licenses/by/4.0/
Keywords: algae; culture experiment; energetics; Laboratory experiment; physiology; protein; sea urchin; Strongylocentrotus purpuratus

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Published: 2024-04-18 • DOI registered: 2024-05-17

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Abstract:

Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.

Keyword(s):

algae; culture experiment; energetics; Laboratory experiment; physiology; protein; sea urchin; Strongylocentrotus purpuratus

Supplement to:

Hildebrand, Jasper; Chang, William Weijen; Hu, Marian Y; Stumpp, Meike (2023): Characterization of digestive proteases in the gut of a basal deuterostome. Journal of Experimental Biology, 226(15), jeb245789, https://doi.org/10.1242/jeb.245789

Funding:

German Research Foundation (DFG), grant/award no. 441084746 : Black box larval physiology - mechanisms of nutrient acquisition and energetics of invertebrate larvae in a changing ocean

Coverage:

Latitude: 32.842674 * Longitude: -117.257767

Event(s):

La_Jolla_S_purpuratus * Latitude: 32.842674 * Longitude: -117.257767 * Location: La Jolla, USA * Method/Device: Sampling by diver (DIVER) * Comment: Strongylocentrotus purpuratus originally collected in November 2020

Comment:

Determination of the in vivo digestion rate determined by fluorescence microscopy under the influence of protease inhibitors.

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The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.

Parameter(s):

#	Name	Short Name	Principal Investigator	Method/Device	Comment
1	Experiment	Exp	Hildebrand, Jasper		ID
2	Sampling date/time, experiment	Date/time sampling exp	Hildebrand, Jasper
3	Sample ID	Sample ID	Hildebrand, Jasper
4	Type of study	Study type	Hildebrand, Jasper
5	Laboratory	Lab	Hildebrand, Jasper
6	Treatment	Treat	Hildebrand, Jasper
7	Strongylocentrotus purpuratus, larvae, normalized digestion rate	S. purpuratus larv NDR	Hildebrand, Jasper	Calculated	Mean (n=3). Calculated from flourescence intensity.

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

336 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp	2 Date/time sampling exp	3 Sample ID	4 Study type	5 Lab	6 Treat	7 S. purpuratus larv NDR
6	2022-04-04	Spl. 1	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0020
6	2022-04-04	Spl. 2	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0038
6	2022-04-04	Spl. 3	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0017
6	2022-04-04	Spl. 4	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0030
6	2022-04-04	Spl. 5	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0027
6	2022-04-04	Spl. 6	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0021
6	2022-04-04	Spl. 7	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0026
6	2022-04-04	Spl. 8	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0029
6	2022-04-04	Spl. 9	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0027
6	2022-04-04	Spl. 10	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0017
6	2022-04-04	Spl. 11	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0019
6	2022-04-04	Spl. 12	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0030
6	2022-04-04	Spl. 13	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0020
6	2022-04-04	Spl. 14	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0025
6	2022-04-04	Spl. 15	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0026
6	2022-04-04	Spl. 16	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Control	0.0035
6	2022-04-04	Spl. 1	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0029
6	2022-04-04	Spl. 2	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0022
6	2022-04-04	Spl. 3	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0018
6	2022-04-04	Spl. 4	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0028
6	2022-04-04	Spl. 5	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0044
6	2022-04-04	Spl. 6	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0031
6	2022-04-04	Spl. 7	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0024
6	2022-04-04	Spl. 8	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0025
6	2022-04-04	Spl. 9	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0041
6	2022-04-04	Spl. 10	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0025
6	2022-04-04	Spl. 11	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0020
6	2022-04-04	Spl. 12	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0030
6	2022-04-04	Spl. 13	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0035
6	2022-04-04	Spl. 14	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0030
6	2022-04-04	Spl. 15	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0054
6	2022-04-04	Spl. 16	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	Aprotinin [10 µmol/l]	0.0012
6	2022-04-04	Spl. 1	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0056
6	2022-04-04	Spl. 2	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0031
6	2022-04-04	Spl. 3	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0020
6	2022-04-04	Spl. 4	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0038
6	2022-04-04	Spl. 5	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0022
6	2022-04-04	Spl. 6	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0017
6	2022-04-04	Spl. 7	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0031
6	2022-04-04	Spl. 8	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0039
6	2022-04-04	Spl. 9	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0052
6	2022-04-04	Spl. 10	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0033
6	2022-04-04	Spl. 11	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0040
6	2022-04-04	Spl. 12	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0038
6	2022-04-04	Spl. 13	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0025
6	2022-04-04	Spl. 14	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0021
6	2022-04-04	Spl. 15	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0040
6	2022-04-04	Spl. 16	Laboratory experiment	University of Kiel, Zoological Institut, Kiel, Germany	SBTI [100 µg/ml]	0.0028