Hildebrand, Jasper; Chang, William Weijen; Hu, Marian Y; Stumpp, Meike (2024): Protease characterization of larval Strongylocentrotus purpuratus collected from La Jolla, USA [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.966001 (DOI registration in progress)
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Abstract:
Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
Keyword(s):
Supplement to:
Hildebrand, Jasper; Chang, William Weijen; Hu, Marian Y; Stumpp, Meike (2023): Characterization of digestive proteases in the gut of a basal deuterostome. Journal of Experimental Biology, 226(15), jeb245789, https://doi.org/10.1242/jeb.245789
Funding:
German Research Foundation (DFG), grant/award no. 441084746: Black box larval physiology - mechanisms of nutrient acquisition and energetics of invertebrate larvae in a changing ocean
Coverage:
Latitude: 32.842674 * Longitude: -117.257767
Comment:
The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
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16 datasets
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Datasets listed in this bundled publication
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Gene expression of protease genes in larval Strongylocentrotus purpuratus during laboratory experiments with different food treatments. https://doi.pangaea.de/10.1594/PANGAEA.965918
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with different food treatments. https://doi.pangaea.de/10.1594/PANGAEA.965967
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments under different pH conditions. https://doi.pangaea.de/10.1594/PANGAEA.965968
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with different aprotinin treatments. https://doi.pangaea.de/10.1594/PANGAEA.965969
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with different soybean trypsin inhibitor (SBTI) treatments. https://doi.pangaea.de/10.1594/PANGAEA.965970
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Digestion rate in larval Strongylocentrotus purpuratus during laboratory experiments with low protease inhibitor treatments. https://doi.pangaea.de/10.1594/PANGAEA.965971
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Digestion rate in larval Strongylocentrotus purpuratus during laboratory experiments with high protease inhibitor treatments. https://doi.pangaea.de/10.1594/PANGAEA.965972
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Fluorescence intensity in the larval Strongylocentrotus purpuratus midgut during laboratory experiments. https://doi.pangaea.de/10.1594/PANGAEA.965973
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Digestion rates in larval Strongylocentrotus purpuratus during different larval feeding stages. https://doi.pangaea.de/10.1594/PANGAEA.965975
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Immoblization of Rhodomonas sp. during laboratory experiments with different trypsin treatments. https://doi.pangaea.de/10.1594/PANGAEA.965976
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Larval size of Strongylocentrotus purpuratus during laboratory experiments with different food treatments. https://doi.pangaea.de/10.1594/PANGAEA.965977
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Larval number of Strongylocentrotus purpuratus during laboratory experiments with different food treatments. https://doi.pangaea.de/10.1594/PANGAEA.965978
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Larval number of Strongylocentrotus purpuratus during laboratory experiments with high food treatment. https://doi.pangaea.de/10.1594/PANGAEA.965979
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with aprotinin treatment over time. https://doi.pangaea.de/10.1594/PANGAEA.965981
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with soybean trypsin inhibitor (SBTI) treatment over time. https://doi.pangaea.de/10.1594/PANGAEA.965982
- Hildebrand, J; Chang, WW; Hu, MY et al. (2024): Trypsin activity during laboratory experiments with different protease inhibitor and pH treatments. https://doi.pangaea.de/10.1594/PANGAEA.965983