Hildebrand, Jasper; Chang, William Weijen; Hu, Marian Y; Stumpp, Meike (2024): Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with different aprotinin treatments [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.965969, In: Hildebrand, J et al. (2024): Protease characterization of larval Strongylocentrotus purpuratus collected from La Jolla, USA [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.966001
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Abstract:
Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.
Keyword(s):
Supplement to:
Hildebrand, Jasper; Chang, William Weijen; Hu, Marian Y; Stumpp, Meike (2023): Characterization of digestive proteases in the gut of a basal deuterostome. Journal of Experimental Biology, 226(15), jeb245789, https://doi.org/10.1242/jeb.245789
Funding:
German Research Foundation (DFG), grant/award no. 441084746: Black box larval physiology - mechanisms of nutrient acquisition and energetics of invertebrate larvae in a changing ocean
Coverage:
Latitude: 32.842674 * Longitude: -117.257767
Event(s):
Comment:
Determination of residual total protease activity by spectroscopy after addition of the protease inhibitor aprotinin.
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The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Hildebrand, Jasper | ID | ||
| 2 | Sampling date/time, experiment | Date/time sampling exp | Hildebrand, Jasper | |||
| 3 | Sample ID | Sample ID | Hildebrand, Jasper | |||
| 4 | Type of study | Study type | Hildebrand, Jasper | |||
| 5 | Laboratory | Lab | Hildebrand, Jasper | |||
| 6 | Treatment: aprotinin | T:aprotinin | µmol/l | Hildebrand, Jasper | ||
| 7 | Strongylocentrotus purpuratus, larvae, total protease activity, difference in absorption per time and protein mass | S. purpuratus larv TPA | 1/h/mg | Hildebrand, Jasper | Calculated | Absorbance measured with BioSpectrometer (Eppendorf, Hamburg, Germany) at 366 nm. Mean (n=3). |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
42 data points
Data
| 1 Exp | 2 Date/time sampling exp | 3 Sample ID | 4 Study type | 5 Lab | 6 T:aprotinin [µmol/l] | 7 S. purpuratus larv TPA [1/h/mg] |
|---|---|---|---|---|---|---|
| 4 | 2022-01-04 | Spl. 1 | Laboratory experiment | University of Kiel, Zoological Institut, Kiel, Germany | 10.0000 | 0.194 |
| 4 | 2022-01-04 | Spl. 1 | Laboratory experiment | University of Kiel, Zoological Institut, Kiel, Germany | 1.0000 | 0.192 |
| 4 | 2022-01-04 | Spl. 1 | Laboratory experiment | University of Kiel, Zoological Institut, Kiel, Germany | 0.1000 | 0.210 |
| 4 | 2022-01-04 | Spl. 1 | Laboratory experiment | University of Kiel, Zoological Institut, Kiel, Germany | 0.0100 | 0.261 |
| 4 | 2022-01-04 | Spl. 1 | Laboratory experiment | University of Kiel, Zoological Institut, Kiel, Germany | 0.0010 | 0.379 |
| 4 | 2022-01-04 | Spl. 1 | Laboratory experiment | University of Kiel, Zoological Institut, Kiel, Germany | 0.0001 | 0.380 |