Schauberger, Clemens; Middelboe, Mathias; Glud, Ronnie N (2024): Quantification of microbial cells and virus-like particles in the water column above Kermadec Trench [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.965925
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Published: 2024-03-04 • DOI registered: 2024-04-02
Abstract:
Water samples were collected using 7.5-Liter Niskin bottles and fixed with 25% electron microscopy graded glutaraldehyde (1% final concentration) and stored at -80°C until quantification by flow cytometry. Samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I (Brussaard 2004). The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021).
Keyword(s):
Related to:
Schauberger, Clemens; Middelboe, Mathias; Larsen, Morten; Peoples, Logan M; Bartlett, Douglas H; Kirpekar, Finn; Rowden, Ashley A; Wenzhöfer, Frank; Thamdrup, Bo; Glud, Ronnie N (2021): Spatial variability of prokaryotic and viral abundances in the Kermadec and Atacama Trench regions. Limnology and Oceanography, 66(6), 2095-2109, https://doi.org/10.1002/lno.11711
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Median Latitude: -30.768067 * Median Longitude: -176.738667 * South-bound Latitude: -31.935700 * West-bound Longitude: -177.291300 * North-bound Latitude: -29.228300 * East-bound Longitude: -176.116700
Date/Time Start: 2017-12-06T08:28:01 * Date/Time End: 2017-12-09T12:57:01
Minimum DEPTH, water: 5 m * Maximum DEPTH, water: 9000 m
Event(s):
TAN1711_K2 (Site K2) * Latitude: -29.228300 * Longitude: -176.116700 * Date/Time: 2017-12-06T08:28:01 * Elevation: -9700.0 m * Location: Kermadec Trench * Campaign: TAN1711 * Basis: Tangaroa * Method/Device: CTD/Rosette (CTD-RO)
TAN1711_K4-2 (Site K4) * Latitude: -31.140200 * Longitude: -176.808000 * Date/Time: 2017-12-08T02:40:01 * Elevation: -9300.0 m * Location: Kermadec Trench * Campaign: TAN1711 * Basis: Tangaroa * Method/Device: CTD/Rosette (CTD-RO)
TAN1711_K5-2 (Site K5) * Latitude: -31.935700 * Longitude: -177.291300 * Date/Time: 2017-12-09T12:57:01 * Elevation: -10010.0 m * Location: Kermadec Trench * Campaign: TAN1711 * Basis: Tangaroa * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Station label | Station | Schauberger, Clemens | |||
| 3 | Latitude of event | Latitude | ||||
| 4 | Longitude of event | Longitude | ||||
| 5 | Date/Time of event | Date/Time | ||||
| 6 | Elevation of event | Elevation | m | |||
| 7 | DEPTH, water | Depth water | m | Schauberger, Clemens | Geocode | |
| 8 | Microbial abundance, cells | Microbial abund | #/ml | Schauberger, Clemens | Flow cytometry system, Becton Dickinson, FACSCanto II | |
| 9 | Microbial abundance, standard deviation | Microbial abund std dev | ± | Schauberger, Clemens | ||
| 10 | Viral abundance | Virus | #/ml | Schauberger, Clemens | Flow cytometry system, Becton Dickinson, FACSCanto II | |
| 11 | Viral abundance, standard deviation | Virus std dev | ± | Schauberger, Clemens |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
234 data points