Schauberger, Clemens; Middelboe, Mathias; Glud, Ronnie N (2024): Quantification of microbial cells and virus-like particles in the water column above Kermadec Trench [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.965925
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Published: 2024-03-04 • DOI registered: 2024-04-02
Abstract:
Water samples were collected using 7.5-Liter Niskin bottles and fixed with 25% electron microscopy graded glutaraldehyde (1% final concentration) and stored at -80°C until quantification by flow cytometry. Samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I (Brussaard 2004). The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021).
Keyword(s):
Related to:
Schauberger, Clemens; Middelboe, Mathias; Larsen, Morten; Peoples, Logan M; Bartlett, Douglas H; Kirpekar, Finn; Rowden, Ashley A; Wenzhöfer, Frank; Thamdrup, Bo; Glud, Ronnie N (2021): Spatial variability of prokaryotic and viral abundances in the Kermadec and Atacama Trench regions. Limnology and Oceanography, 66(6), 2095-2109, https://doi.org/10.1002/lno.11711
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Median Latitude: -30.768067 * Median Longitude: -176.738667 * South-bound Latitude: -31.935700 * West-bound Longitude: -177.291300 * North-bound Latitude: -29.228300 * East-bound Longitude: -176.116700
Date/Time Start: 2017-12-06T08:28:01 * Date/Time End: 2017-12-09T12:57:01
Minimum DEPTH, water: 5 m * Maximum DEPTH, water: 9000 m
Event(s):
TAN1711_K2 (Site K2) * Latitude: -29.228300 * Longitude: -176.116700 * Date/Time: 2017-12-06T08:28:01 * Elevation: -9700.0 m * Location: Kermadec Trench * Campaign: TAN1711 * Basis: Tangaroa * Method/Device: CTD/Rosette (CTD-RO)
TAN1711_K4-2 (Site K4) * Latitude: -31.140200 * Longitude: -176.808000 * Date/Time: 2017-12-08T02:40:01 * Elevation: -9300.0 m * Location: Kermadec Trench * Campaign: TAN1711 * Basis: Tangaroa * Method/Device: CTD/Rosette (CTD-RO)
TAN1711_K5-2 (Site K5) * Latitude: -31.935700 * Longitude: -177.291300 * Date/Time: 2017-12-09T12:57:01 * Elevation: -10010.0 m * Location: Kermadec Trench * Campaign: TAN1711 * Basis: Tangaroa * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Station label | Station | Schauberger, Clemens | |||
| 3 | Latitude of event | Latitude | ||||
| 4 | Longitude of event | Longitude | ||||
| 5 | Date/Time of event | Date/Time | ||||
| 6 | Elevation of event | Elevation | m | |||
| 7 | DEPTH, water | Depth water | m | Schauberger, Clemens | Geocode | |
| 8 | Microbial abundance, cells | Microbial abund | #/ml | Schauberger, Clemens | Flow cytometry system, Becton Dickinson, FACSCanto II | |
| 9 | Microbial abundance, standard deviation | Microbial abund std dev | ± | Schauberger, Clemens | ||
| 10 | Viral abundance | Virus | #/ml | Schauberger, Clemens | Flow cytometry system, Becton Dickinson, FACSCanto II | |
| 11 | Viral abundance, standard deviation | Virus std dev | ± | Schauberger, Clemens |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
234 data points
Data
| 1 Event | 2 Station | 3 Latitude | 4 Longitude | 5 Date/Time | 6 Elevation [m] | 7 Depth water [m] | 8 Microbial abund [#/ml] | 9 Microbial abund std dev [±] | 10 Virus [#/ml] | 11 Virus std dev [±] |
|---|---|---|---|---|---|---|---|---|---|---|
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 5 | 1.07E006 | 2.77E004 | 9.64E005 | 2.39E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 50 | 4.74E005 | 5.38E004 | 1.29E006 | 1.91E005 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 250 | 3.01E005 | 3.34E003 | 4.36E005 | 4.49E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 500 | 1.73E005 | 1.16E004 | 2.77E005 | 4.09E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 1500 | 6.81E004 | 3.25E003 | 8.47E004 | 8.69E003 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 2000 | 5.67E003 | 6.65E005 | 1.88E004 | |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 3000 | 4.76E004 | 4.50E003 | 7.68E004 | 1.69E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 3500 | 5.14E004 | 2.72E003 | 8.86E004 | 2.01E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 4000 | 3.35E004 | 4.05E003 | 1.05E005 | 1.43E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 4500 | 3.03E004 | 4.92E002 | 1.28E005 | 2.64E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 5000 | 3.56E004 | 2.26E003 | 1.31E005 | 3.08E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 5500 | 4.53E004 | 3.70E003 | 1.29E005 | 1.34E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 6000 | 6.53E004 | 4.49E003 | 1.07E005 | 1.24E003 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 7500 | 1.38E004 | 3.92E002 | 9.46E004 | 3.90E003 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 8000 | 1.62E004 | 2.98E003 | 9.18E004 | 5.32E003 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 8500 | 1.52E004 | 2.49E003 | 1.03E005 | 8.69E004 |
| TAN1711_K2 | Site K2 | -29.2283 | -176.1167 | 2017-12-06T08:28:01 | -9700 | 9000 | 1.69E004 | 4.12E003 | 1.19E005 | 4.17E004 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 50 | 7.53E005 | 7.75E004 | 6.86E005 | 8.51E004 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 250 | 2.80E005 | 1.12E004 | 1.96E005 | 1.31E004 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 500 | 1.73E005 | 3.96E003 | 1.34E005 | 6.57E002 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 1500 | 4.46E004 | 1.67E003 | 6.03E004 | 1.75E003 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 2000 | 3.75E004 | 2.46E003 | 7.06E004 | 2.18E003 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 3000 | 2.75E004 | 1.13E003 | 3.56E004 | 3.58E003 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 3500 | 3.03E004 | 1.81E003 | 8.92E004 | 2.74E003 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 4000 | 3.54E004 | 6.29E002 | 8.65E004 | 1.24E004 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 4500 | 2.55E004 | 5.80E002 | 8.09E004 | 8.57E003 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 5000 | 2.59E004 | 3.27E003 | 8.50E004 | 1.08E004 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 5500 | 3.07E004 | 3.45E003 | 1.13E005 | 9.10E003 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 6000 | 5.40E004 | 1.27E003 | 7.15E004 | 3.46E003 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 7000 | 9.90E003 | 2.99E002 | 7.27E004 | 7.10E003 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 8000 | 1.03E004 | 7.28E002 | 6.57E004 | 2.24E004 |
| TAN1711_K4-2 | Site K4 | -31.1402 | -176.8080 | 2017-12-08T02:40:01 | -9300 | 8700 | 1.14E004 | 8.69E002 | 6.48E004 | 2.25E003 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 50 | 8.48E005 | 6.66E004 | 1.69E006 | 9.34E004 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 250 | 3.58E005 | 4.74E004 | 4.81E005 | 4.71E004 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 500 | 2.02E005 | 2.94E004 | 1.83E005 | 4.41E004 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 1500 | 6.59E004 | 1.19E004 | 8.18E004 | 1.98E004 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 2000 | 8.29E004 | 1.14E004 | 9.11E004 | 2.24E003 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 3000 | 5.26E004 | 3.64E003 | 7.56E004 | 2.57E003 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 3500 | 3.82E004 | 4.42E003 | 8.33E004 | 6.62E003 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 4000 | 3.13E004 | 1.47E003 | 9.21E004 | 2.27E003 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 4500 | 3.18E004 | 7.36E003 | 8.73E004 | 1.73E004 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 5000 | 2.53E004 | 4.01E003 | 1.03E005 | 4.44E003 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 5500 | 3.20E004 | 7.52E002 | 9.84E004 | 1.61E004 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 6000 | 3.73E004 | 1.48E003 | 1.04E005 | 1.66E003 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 7000 | 1.51E004 | 1.36E003 | 8.27E004 | 7.33E003 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 8000 | 2.31E004 | 2.20E003 | 8.34E004 | 4.09E002 |
| TAN1711_K5-2 | Site K5 | -31.9357 | -177.2913 | 2017-12-09T12:57:01 | -10010 | 8700 | 3.28E004 | 8.43E002 | 1.00E005 | 1.35E004 |