Schauberger, Clemens; Middelboe, Mathias; Glud, Ronnie N (2024): Quantification of microbial cells and virus-like particles in the water column above Atacama Trench [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.965921
Always quote citation above when using data! You can download the citation in several formats below.
Published: 2024-03-04 • DOI registered: 2024-04-02
Abstract:
Water samples were collected using 7.5-Liter Niskin bottles and fixed with 25% electron microscopy graded glutaraldehyde (1% final concentration) and stored at -80°C until quantification by flow cytometry. Samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I (Brussaard 2004). The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021).
Related to:
Schauberger, Clemens; Middelboe, Mathias; Larsen, Morten; Peoples, Logan M; Bartlett, Douglas H; Kirpekar, Finn; Rowden, Ashley A; Wenzhöfer, Frank; Thamdrup, Bo; Glud, Ronnie N (2021): Spatial variability of prokaryotic and viral abundances in the Kermadec and Atacama Trench regions. Limnology and Oceanography, 66(6), 2095-2109, https://doi.org/10.1002/lno.11711
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Median Latitude: -22.668605 * Median Longitude: -71.353633 * South-bound Latitude: -24.284033 * West-bound Longitude: -72.145983 * North-bound Latitude: -20.304967 * East-bound Longitude: -70.836067
Date/Time Start: 2018-03-06T03:01:00 * Date/Time End: 2018-03-28T06:23:00
Minimum DEPTH, water: 5 m * Maximum DEPTH, water: 7700 m
Event(s):
SO261_7-2 * Latitude: -23.820800 * Longitude: -70.836067 * Date/Time: 2018-03-06T03:01:00 * Elevation: -2549.1 m * Campaign: SO261 * Basis: Sonne_2 * Method/Device: CTD/Rosette (CTD-RO)
SO261_19-1 * Latitude: -24.284033 * Longitude: -71.423350 * Date/Time: 2018-03-07T23:38:00 * Elevation: -7830.4 m * Campaign: SO261 * Basis: Sonne_2 * Method/Device: CTD/Rosette (CTD-RO) * Comment: Hadal-Rosette
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Station label | Station | Schauberger, Clemens | |||
| 3 | Latitude of event | Latitude | ||||
| 4 | Longitude of event | Longitude | ||||
| 5 | Date/Time of event | Date/Time | ||||
| 6 | Elevation of event | Elevation | m | |||
| 7 | DEPTH, water | Depth water | m | Schauberger, Clemens | Geocode | |
| 8 | Microbial abundance, cells | Microbial abund | #/ml | Schauberger, Clemens | Flow cytometry system, Becton Dickinson, FACSCanto II | |
| 9 | Microbial abundance, standard deviation | Microbial abund std dev | ± | Schauberger, Clemens | ||
| 10 | Viral abundance | Virus | #/ml | Schauberger, Clemens | Flow cytometry system, Becton Dickinson, FACSCanto II | |
| 11 | Viral abundance, standard deviation | Virus std dev | ± | Schauberger, Clemens |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
658 data points