Khalil, Munawar; Stuhr, Marleen; Kunzmann, Andreas; Westphal, Hildegard: Ca-ATPase and Mg-ATPase enzyme activities of the tropical-subtropical starfish Aquilonastra yairi under ocean acidification and warming in the laboratory [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.965905 (dataset in review)
Abstract:
Enzyme activities involved in biomineralization were determined in the tropical-subtropical starfish Aquilonastra yairi from the culture stock of the ZMT-MAREE laboratory in response to projected near-future global change. To investigate the physicochemical alteration of starfish induced by elevated seawater pCO2 and temperature, starfish of the species A. yairi (342 specimens, size 3-11 mm in diameter, culture stock) were reared in aquaria system under controlled experimental environment conditions at ZMT-MAREE. The starfish were acclimatized to two temperature levels (27 °C, 32 °C) crossed with three pCO2 concentrations (455 µatm, 1052 µatm, 2066 µatm). Protein concentration was determined using the Bradford assay method (Bradford, 1976). Furthermore, Ca-ATPase and Mg-ATPase activities were measured according to protocols initially developed by Chan et al. (1986), Busacker and Chavin (1981) and modified by Prazeres et al. (2015). Positive parabolic responses of Ca-ATPase and Mg-ATPase enzyme activities were detected at 27 °C with elevated pCO2, while stable enzyme activities were observed at 32 °C with elevated pCO2. The calcification-related enzymes Ca-ATPase and Mg-ATPase activity appear to be sensitive to pCO2/pH changes, leading to vulnerability concerning the skeletal structure.
References:
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Busacker, Greg P; Chavin, Walter (1981): Characterization of and Mg2+-ATPases from the gill and the kidney of the goldfish (Carassius auratus L.). Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 69(2), 249-256, https://doi.org/10.1016/0305-0491(81)90237-6
Chan, Kwok-Ming; Delfert, Dennis; Junger, Kurt D (1986): A direct colorimetric assay for Ca2+-stimulated ATPase activity. Analytical Biochemistry, 157(2), 375-380, https://doi.org/10.1016/0003-2697(86)90640-8
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Prazeres, Martina; Uthicke, Sven; Pandolfi, John M (2015): Ocean acidification induces biochemical and morphological changes in the calcification process of large benthic foraminifera. Proceedings of the Royal Society B-Biological Sciences, 282(1803), 20142782-20142782, https://doi.org/10.1098/rspb.2014.2782
Parameter(s):
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)
Size:
1749 data points