Citation:

PANGAEA.965905

Name: Ca-ATPase and Mg-ATPase enzyme activities of the tropical-subtropical starfish Aquilonastra yairi under ocean acidification and warming in the laboratory
Published: 2024-05-14
License: https://creativecommons.org/licenses/by/4.0/
Keywords: enzyme activity; global change; Laboratory experiment; starfish

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Abstract:

Enzyme activities involved in biomineralization were determined in the tropical-subtropical starfish Aquilonastra yairi from the culture stock of the ZMT-MAREE laboratory in response to projected near-future global change. To investigate the physicochemical alteration of starfish induced by elevated seawater pCO2 and temperature, starfish of the species A. yairi (342 specimens, size 3-11 mm in diameter, culture stock) were reared in aquaria system under controlled experimental environment conditions at ZMT-MAREE. The starfish were acclimatized to two temperature levels (27 °C, 32 °C) crossed with three pCO2 concentrations (455 µatm, 1052 µatm, 2066 µatm). Protein concentration was determined using the Bradford assay method (Bradford, 1976). Furthermore, Ca-ATPase and Mg-ATPase activities were measured according to protocols initially developed by Chan et al. (1986), Busacker and Chavin (1981) and modified by Prazeres et al. (2015). Positive parabolic responses of Ca-ATPase and Mg-ATPase enzyme activities were detected at 27 °C with elevated pCO2, while stable enzyme activities were observed at 32 °C with elevated pCO2. The calcification-related enzymes Ca-ATPase and Mg-ATPase activity appear to be sensitive to pCO2/pH changes, leading to vulnerability concerning the skeletal structure.

Keyword(s):

enzyme activity; global change; Laboratory experiment; starfish

Supplement to:

Khalil, Munawar; Stuhr, Marleen; Kunzmann, Andreas; Westphal, Hildegard (2024): Simultaneous ocean acidification and warming do not alter the lipid-associated biochemistry but induce enzyme activities in an asterinid starfish. Science of the Total Environment, 932, 173000, https://doi.org/10.1016/j.scitotenv.2024.173000

References:

Bradford, Marion M (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254, https://doi.org/10.1016/0003-2697(76)90527-3

Busacker, Greg P; Chavin, Walter (1981): Characterization of and Mg2+-ATPases from the gill and the kidney of the goldfish (Carassius auratus L.). Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 69(2), 249-256, https://doi.org/10.1016/0305-0491(81)90237-6

Chan, Kwok-Ming; Delfert, Dennis; Junger, Kurt D (1986): A direct colorimetric assay for Ca2+-stimulated ATPase activity. Analytical Biochemistry, 157(2), 375-380, https://doi.org/10.1016/0003-2697(86)90640-8

Dickson, Andrew G; Sabine, Christopher L; Christian, J R (2007): Guide to Best Practices for Ocean CO2 Measurements. PICES Special Publication, 3, 191 pp, hdl:10013/epic.51789.d001

Pierrot, D E; Lewis, E; Wallace, D W R (2006): MS Excel Program Developed for CO2 System Calculations. Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, U.S. Department of Energy, https://doi.org/10.3334/CDIAC/otg.CO2SYS_XLS_CDIAC105a

Prazeres, Martina; Uthicke, Sven; Pandolfi, John M (2015): Ocean acidification induces biochemical and morphological changes in the calcification process of large benthic foraminifera. Proceedings of the Royal Society B-Biological Sciences, 282(1803), 20142782-20142782, https://doi.org/10.1098/rspb.2014.2782

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device
1	Type of study	Study type		Khalil, Munawar
2	Species, unique identification	Species UID		Khalil, Munawar
3	Species, unique identification (URI)	Species UID (URI)		Khalil, Munawar
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Khalil, Munawar
5	Treatment	Treat		Khalil, Munawar
6	Sampling date/time, experiment	Date/time sampling exp		Khalil, Munawar
7	Treatment: temperature	T:temp	°C	Khalil, Munawar	Multi-parameter portable meter, Xylem Analytics, WTW MultiLine® 3630 IDS
8	Treatment: partial pressure of carbon dioxide	T:pCO2	µatm	Khalil, Munawar	calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006)
9	pH	pH		Khalil, Munawar	Spectrophotometer; according to Dickson et al. (2007)
10	pH, standard deviation	pH std dev	±	Khalil, Munawar	Calculated
11	Sample ID	Sample ID		Khalil, Munawar
12	Calcium adenosine triphosphatase activity	Ca-ATPase act	µmol/mg/min	Khalil, Munawar	Spectrophotometry
13	Magnesium adenosine triphosphatase activity	Mg-ATPase act	µmol/mg/min	Khalil, Munawar	Spectrophotometry

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)

Size:

1749 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Treat	6 Date/time sampling exp	7 T:temp [°C]	8 T:pCO2 [µatm]	9 pH	10 pH std dev [±]	11 Sample ID	12 Ca-ATPase act [µmol/mg/min]	13 Mg-ATPase act [µmol/mg/min]
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2020-12-23	27	455	8.00	0.00	A1.1.(E1)	0.735	0.878
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2020-12-23	27	455	8.00	0.00	A1.1.(E1)	0.842	0.827
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2020-12-23	27	455	8.00	0.00	A1.1.(E1)	0.922	1.026
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2020-12-23	27	455	8.00	0.00	A1.2.(E1)	0.977	1.052
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2020-12-23	27	455	8.00	0.00	A1.2.(E1)	1.069	1.052
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2020-12-23	27	455	8.00	0.00	A1.2.(E1)	1.034	1.073
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2020-12-23	27	455	8.00	0.00	A1.3.(E1)	0.803	0.796
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2020-12-23	27	455	8.00	0.00	A1.3.(E1)	0.830	0.790
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2020-12-23	27	455	8.00	0.00	A1.3.(E1)	0.955	0.895
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2020-12-23	27	1052	7.74	0.01	A2.1.(E1)	0.586	0.577
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2020-12-23	27	1052	7.74	0.01	A2.1.(E1)	0.631	0.640
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2020-12-23	27	1052	7.74	0.01	A2.1.(E1)	0.613	0.656
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2020-12-23	27	1052	7.74	0.01	A2.2.(E1)	0.495	0.464
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2020-12-23	27	1052	7.74	0.01	A2.2.(E1)	0.525	0.494
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2020-12-23	27	1052	7.74	0.01	A2.2.(E1)	0.507	0.514
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2020-12-23	27	1052	7.74	0.01	A2.3.(E1)	0.539	0.571
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2020-12-23	27	1052	7.74	0.01	A2.3.(E1)	0.486	0.526
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2020-12-23	27	1052	7.74	0.01	A2.3.(E1)	0.545	0.539
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2020-12-23	27	2066	7.74	0.01	A3.1.(E1)	0.708	0.689
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2020-12-23	27	2066	7.47	0.01	A3.1.(E1)	0.670	0.652
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2020-12-23	27	2066	7.47	0.01	A3.1.(E1)	0.700	0.685
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2020-12-23	27	2066	7.74	0.02	A3.2.(E1)	0.915	0.935
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2020-12-23	27	2066	7.47	0.01	A3.2.(E1)	0.652	0.819
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2020-12-23	27	2066	7.47	0.01	A3.2.(E1)	0.982	0.891
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2020-12-23	27	2066	7.74	0.03	A3.3.(E1)	1.092	1.054
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2020-12-23	27	2066	7.47	0.01	A3.3.(E1)	0.867	0.884
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2020-12-23	27	2066	7.47	0.01	A3.3.(E1)	0.860	0.906
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2020-12-23	32	455	8.00	0.00	B1.1.(E1)	0.865	0.986
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2020-12-23	32	455	8.00	0.00	B1.1.(E1)	1.021	1.016
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2020-12-23	32	455	8.00	0.00	B1.1.(E1)	0.965	0.995
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2020-12-23	32	455	8.00	0.00	B1.2.(E1)	0.590	0.577
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2020-12-23	32	455	8.00	0.00	B1.2.(E1)	0.614	0.558
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2020-12-23	32	455	8.00	0.00	B1.2.(E1)	0.629	0.627
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2020-12-23	32	455	8.00	0.00	B1.3.(E1)	1.159	0.994
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2020-12-23	32	455	8.00	0.00	B1.3.(E1)	1.057	0.961
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2020-12-23	32	455	8.00	0.00	B1.3.(E1)	1.143	1.011
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2020-12-23	32	1052	7.74	0.00	B2.1.(E1)	0.907	0.758
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2020-12-23	32	1052	7.74	0.00	B2.1.(E1)	0.848	0.830
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2020-12-23	32	1052	7.74	0.00	B2.1.(E1)	0.776	0.814
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2020-12-23	32	1052	7.74	0.00	B2.2.(E1)	0.652	0.635
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2020-12-23	32	1052	7.74	0.00	B2.2.(E1)	0.634	0.681
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2020-12-23	32	1052	7.74	0.00	B2.2.(E1)	0.653	0.633
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2020-12-23	32	1052	7.74	0.00	B2.3.(E1)	0.640	0.605
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2020-12-23	32	1052	7.74	0.00	B2.3.(E1)	0.755	0.690
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2020-12-23	32	1052	7.74	0.00	B2.3.(E1)	0.671	0.636
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2020-12-23	32	2066	7.47	0.01	B3.1.(E1)	0.881	0.775
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2020-12-23	32	2066	7.47	0.01	B3.1.(E1)	0.843	0.755
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2020-12-23	32	2066	7.47	0.01	B3.1.(E1)	0.872	0.750
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2020-12-23	32	2066	7.47	0.01	B3.2.(E1)	0.714	0.696
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2020-12-23	32	2066	7.47	0.01	B3.2.(E1)	0.617	0.632
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2020-12-23	32	2066	7.47	0.01	B3.2.(E1)	0.625	0.626
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2020-12-23	32	2066	7.47	0.01	B3.3.(E1)	0.768	0.691
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2020-12-23	32	2066	7.47	0.01	B3.3.(E1)	0.629	0.589
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2020-12-23	32	2066	7.47	0.01	B3.3.(E1)	0.666	0.622
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-01-26	32	455	8.00	0.00	A1.1.(E2)	0.650	0.593
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-01-26	32	455	8.00	0.00	A1.1.(E2)	0.606	0.598
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-01-26	32	455	8.00	0.00	A1.1.(E2)	0.598	0.652
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-01-26	32	455	8.00	0.00	A1.2.(E2)	0.649	0.694
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-01-26	32	455	8.00	0.00	A1.2.(E2)	0.702	0.709
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-01-26	32	455	8.00	0.00	A1.2.(E2)	0.670	0.687
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-01-26	32	455	8.00	0.00	A1.3.(E2)	0.525	0.531
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-01-26	32	455	8.00	0.00	A1.3.(E2)	0.508	0.503
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-01-26	32	455	8.00	0.00	A1.3.(E2)	0.496	0.514
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-01-26	32	1052	7.74	0.01	A2.1.(E2)	0.635	0.615
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-01-26	32	1052	7.74	0.01	A2.1.(E2)	0.542	0.549
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-01-26	32	1052	7.74	0.01	A2.1.(E2)	0.612	0.628
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-01-26	32	1052	7.74	0.01	A2.2.(E2)	0.563	0.615
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-01-26	32	1052	7.74	0.01	A2.2.(E2)	0.580	0.529
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-01-26	32	1052	7.74	0.01	A2.2.(E2)	0.598	0.590
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-01-26	32	1052	7.74	0.01	A2.3.(E2)	0.727	0.758
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-01-26	32	1052	7.74	0.01	A2.3.(E2)	0.796	0.768
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-01-26	32	1052	7.74	0.01	A2.3.(E2)	0.759	0.764
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-01-26	32	2066	7.74	0.01	A3.1.(E2)	0.585	0.594
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	A3.1.(E2)	0.550	0.583
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	A3.1.(E2)	0.623	0.660
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-01-26	32	2066	7.74	0.02	A3.2.(E2)	0.595	0.598
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	A3.2.(E2)	0.584	0.563
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	A3.2.(E2)	0.657	0.628
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-01-26	32	2066	7.74	0.03	A3.3.(E2)	1.124	1.043
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	A3.3.(E2)	1.011	0.997
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	A3.3.(E2)	1.148	1.080
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-01-26	32	455	8.00	0.00	B1.2.(E2)	0.315	0.301
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-01-26	32	455	8.00	0.00	B1.2.(E2)	0.313	0.301
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-01-26	32	455	8.00	0.00	B1.2.(E2)	0.364	0.346
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-01-26	32	455	8.00	0.00	B1.3.(E2)	0.551	0.521
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-01-26	32	455	8.00	0.00	B1.3.(E2)	0.531	0.505
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-01-26	32	455	8.00	0.00	B1.3.(E2)	0.569	0.622
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-01-26	32	1052	7.74	0.00	B2.1.(E2)	0.541	0.536
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-01-26	32	1052	7.74	0.00	B2.1.(E2)	0.529	0.571
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-01-26	32	1052	7.74	0.00	B2.1.(E2)	0.505	0.562
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-01-26	32	1052	7.74	0.00	B2.2.(E2)	0.551	0.581
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-01-26	32	1052	7.74	0.00	B2.2.(E2)	0.503	0.529
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-01-26	32	1052	7.74	0.00	B2.2.(E2)	0.531	0.558
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-01-26	32	1052	7.74	0.00	B2.3.(E2)	0.579	0.561
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-01-26	32	1052	7.74	0.00	B2.3.(E2)	0.585	0.577
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-01-26	32	1052	7.74	0.00	B2.3.(E2)	0.668	0.696
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	B3.1.(E2)	0.707	0.723
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	B3.1.(E2)	0.720	0.715
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	B3.1.(E2)	0.804	0.853
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	B3.2.(E2)	0.671	0.717
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	B3.2.(E2)	0.605	0.616
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	B3.2.(E2)	0.627	0.631
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	B3.3.(E2)	0.486	0.488
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	B3.3.(E2)	0.473	0.503
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-01-26	32	2066	7.47	0.01	B3.3.(E2)	0.435	0.444
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-03-08	27	455	8.00	0.00	A1.1.(E3)	0.474	0.453
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-03-08	27	455	8.00	0.00	A1.1.(E3)	0.534	0.521
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-03-08	27	455	8.00	0.00	A1.1.(E3)	0.575	0.577
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-03-08	27	455	8.00	0.00	A1.2.(E3)	0.581	0.588
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-03-08	27	455	8.00	0.00	A1.2.(E3)	0.666	0.636
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-03-08	27	455	8.00	0.00	A1.2.(E3)	0.656	0.668
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-03-08	27	455	8.00	0.00	A1.3.(E3)	0.812	0.853
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-03-08	27	455	8.00	0.00	A1.3.(E3)	0.754	0.797
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 455 µatm	2021-03-08	27	455	8.00	0.00	A1.3.(E3)	0.893	0.875
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-03-08	27	1052	7.74	0.01	A2.1.(E3)	0.557	0.553
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-03-08	27	1052	7.74	0.01	A2.1.(E3)	0.536	0.515
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-03-08	27	1052	7.74	0.01	A2.1.(E3)	0.523	0.527
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-03-08	27	1052	7.74	0.01	A2.2.(E3)	0.500	0.469
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-03-08	27	1052	7.74	0.01	A2.2.(E3)	0.541	0.525
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-03-08	27	1052	7.74	0.01	A2.2.(E3)	0.494	0.479
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-03-08	27	1052	7.74	0.01	A2.3.(E3)	0.661	0.670
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-03-08	27	1052	7.74	0.01	A2.3.(E3)	0.628	0.616
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 1052 µatm	2021-03-08	27	1052	7.74	0.01	A2.3.(E3)	0.692	0.730
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-03-08	27	2066	7.74	0.01	A3.1.(E3)	0.558	0.530
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-03-08	27	2066	7.47	0.01	A3.1.(E3)	0.508	0.538
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-03-08	27	2066	7.47	0.01	A3.1.(E3)	0.518	0.506
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-03-08	27	2066	7.74	0.02	A3.2.(E3)	0.672	0.679
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-03-08	27	2066	7.47	0.01	A3.2.(E3)	0.579	0.614
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-03-08	27	2066	7.47	0.01	A3.2.(E3)	0.644	0.669
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-03-08	27	2066	7.74	0.03	A3.3.(E3)	0.521	0.494
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-03-08	27	2066	7.47	0.01	A3.3.(E3)	0.530	0.543
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	27°C : 2066 µatm	2021-03-08	27	2066	7.47	0.01	A3.3.(E3)	0.508	0.516
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-03-08	32	455	8.00	0.00	B1.1.(E3)	0.581	0.552
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-03-08	32	455	8.00	0.00	B1.1.(E3)	0.575	0.563
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-03-08	32	455	8.00	0.00	B1.1.(E3)	0.567	0.550
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-03-08	32	455	8.00	0.00	B1.2.(E3)	0.582	0.578
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-03-08	32	455	8.00	0.00	B1.2.(E3)	0.591	0.604
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-03-08	32	455	8.00	0.00	B1.2.(E3)	0.593	0.641
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-03-08	32	455	8.00	0.00	B1.3.(E3)	0.644	0.546
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-03-08	32	455	8.00	0.00	B1.3.(E3)	0.695	0.609
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 455 µatm	2021-03-08	32	455	8.00	0.00	B1.3.(E3)	0.666	0.655
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-03-08	32	1052	7.74	0.00	B2.1.(E3)	0.454	0.462
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-03-08	32	1052	7.74	0.00	B2.1.(E3)	0.456	0.486
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-03-08	32	1052	7.74	0.00	B2.1.(E3)	0.462	0.491
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-03-08	32	1052	7.74	0.00	B2.2.(E3)	0.716	0.665
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-03-08	32	1052	7.74	0.00	B2.2.(E3)	0.693	0.670
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-03-08	32	1052	7.74	0.00	B2.2.(E3)	0.712	0.683
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-03-08	32	1052	7.74	0.00	B2.3.(E3)	0.437	0.448
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-03-08	32	1052	7.74	0.00	B2.3.(E3)	0.479	0.477
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 1052 µatm	2021-03-08	32	1052	7.74	0.00	B2.3.(E3)	0.459	0.463
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-03-08	32	2066	7.47	0.01	B3.1.(E3)	0.460	0.484
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-03-08	32	2066	7.47	0.01	B3.1.(E3)	0.434	0.458
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-03-08	32	2066	7.47	0.01	B3.1.(E3)	0.474	0.513
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-03-08	32	2066	7.47	0.01	B3.2.(E3)	0.464	0.466
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-03-08	32	2066	7.47	0.01	B3.2.(E3)	0.512	0.528
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-03-08	32	2066	7.47	0.01	B3.2.(E3)	0.486	0.530
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-03-08	32	2066	7.47	0.01	B3.3.(E3)	0.492	0.476
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-03-08	32	2066	7.47	0.01	B3.3.(E3)	0.592	0.562
Laboratory experiment	Aquilonastra yairi	marinespecies.org	urn:lsid:marinespecies.org:taxname:382644	32°C : 2066 µatm	2021-03-08	32	2066	7.47	0.01	B3.3.(E3)	0.501	0.533