Möller, Kristof; Thoms, Silke; Tillmann, Urban; Krock, Bernd; Koch, Florian; Peeken, Ilka; Meunier, Cédric Léo (2024): Growth response, toxin/pigment/nutrient content and photophysiological parameters of Alexandrium pseudogonyaulax after exposure to different light intensities [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.965197 (DOI registration in progress), In: Möller, K et al. (2024): Effects of bottom-up factors (N-source and light) on growth and toxin content of the harmful dinoflagellate Alexandrium pseudogonyaulax [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.965195
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Abstract:
The effect of different light intensities (20, 100 and 200 μmol photons m-2 s-1) on the growth response, toxin/pigment/nutrient content and photophysiology of Alexandrium pseudogonyaulax, isolated in 2020 from the Danish Limfjord, were investigated by means of a bottle incubation experiment. The experiment was started on the 16th March 2022 and finished on the 14th April 2022. This dataset contains all data collected within the two experiments, including cell counts, cell sizes, goniodomin A toxin contents, particular organic carbon and nitrogen, pigment contents and photophysiological parameters. Chlorophyll a fluorescence measurements were performed using a Fast Repetition Rate fluorometer (FRRf) coupled to a FastAct Laboratory system (FastOcean PTX, both from Chelsea Technologies Group) in the exponential growth phase. The parameters were determined after 10 min of dark acclimation.
Keyword(s):
Related to:
Möller, Kristof; Thoms, Silke; Tillmann, Urban; Krock, Bernd; Koch, Florian; Peeken, Ilka; Meunier, Cédric Léo (2024): Effects of bottom‐up factors on growth and toxin content of a harmful algae bloom dinoflagellate. Limnology and Oceanography, lno.12576, https://doi.org/10.1002/lno.12576
Coverage:
Latitude: 56.630000 * Longitude: 8.290000
Date/Time Start: 2020-08-17T09:10:00 * Date/Time End: 2020-08-17T09:10:00
Event(s):
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Type of study | Study type | Möller, Kristof | |||
2 | Date/time start, experiment | Date/time start exp | Möller, Kristof | |||
3 | Date/time end, experiment | Date/time end exp | Möller, Kristof | |||
4 | Treatment: light intensity | T:Io | µmol/m2/s | Möller, Kristof | ||
5 | Radiation, photosynthetically active | PAR | µmol/m2/s | Möller, Kristof | Light sensor (ULM-500, Walz GmbH, Effeltrich, Germany) | |
6 | Replicate | Repl | Möller, Kristof | |||
7 | Time, incubation | T incubation | day | Möller, Kristof | ||
8 | Alexandrium pseudogonyaulax | A. pseudogonyaulax | #/ml | Möller, Kristof | Visual identification (VID) of Lugol preserved samples | |
9 | Carbon, organic, particulate, per cell | POC/cell | ng/# | Möller, Kristof | Kept in dark at -20°C on precombusted Gf/f filter; dried for 24 h at 60 °C, then added conc. HCl and dried for 24 h at 60 °C; measured with Elemental analyser, HEKAtech, Euro EA 3000 | |
10 | Nitrogen, organic, particulate, per cell | PON/cell | ng/# | Möller, Kristof | Kept in dark at -20°C on precombusted Gf/f filter; dried for 24 h at 60 °C, then added conc. HCl and dried for 24 h at 60 °C; measured with Elemental analyser, HEKAtech, Euro EA 3000 | |
11 | Goniodomin A per cell | GDA/cell | pg/# | Möller, Kristof | Centrifuged off, solubilized in methanol, spin-filtered; measured with ultra-high-performance liquid chromatography (UHPLC); coupled with triple quadrupole mass spectrometer (TQMS), Waters, Xevo TQ-XS | A. pseudogonyaulax cells |
12 | Chlorophyll c1+c2 per cell | Chl c1+c2/cell | pg/# | Möller, Kristof | High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters | |
13 | Peridinin per cell | Perid/cell | pg/# | Möller, Kristof | High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters | |
14 | Violaxanthin per cell | Viola/cell | pg/# | Möller, Kristof | High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters | |
15 | Diadinoxanthin per cell | Diadino/cell | pg/# | Möller, Kristof | High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters | |
16 | Dinoxanthin per cell | Dino/cell | pg/# | Möller, Kristof | High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters | |
17 | Diatoxanthin per cell | Diato/cell | pg/# | Möller, Kristof | High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters | |
18 | Zeaxanthin per cell | Zea/cell | pg/# | Möller, Kristof | High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters | |
19 | Chlorophyll a per cell | Chl a/cell | pg/# | Möller, Kristof | High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters | |
20 | beta-Carotene per cell | b-Car/cell | pg/# | Möller, Kristof | High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters | |
21 | Fluorescence, maximum | Fm | Möller, Kristof | Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX | Fm('): fluorescence when C = 1 (under ambient light) | |
22 | Fluorescence, variable | Fv | Möller, Kristof | Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX | Fv(Fq'): Fm'-F' | |
23 | Quantum yield efficiency of photosystem II | Fv/Fm | Möller, Kristof | Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX | Fv/Fm(Fq'/Fm'): fluorescence parameter providing an estimate of PSII efficiency when C = 0 (under ambient light) = photosynthetic efficiency | |
24 | Connectivity between photosystems PSI and PSII | p | Möller, Kristof | Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX | p('): connectivity between PSI/II | |
25 | Probability of an RCII being closed during the first flashlet of an single turnover saturation phase | RSigma | Möller, Kristof | Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX | RSigma(') | |
26 | Absorption cross section of PSII photochemistry | nm**2/PSII | Möller, Kristof | Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX | Sigma(') | |
27 | Time constant for the initial part of an single turnover relaxation phase | Tau | µs | Möller, Kristof | Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX | Tau(') |
28 | Functional photosystem II reaction centers | RCII | nmol/m3 | Möller, Kristof | Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX | [RCII] |
29 | Maximal electron transport rate, relative | rETR max | Möller, Kristof | Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX | maxETR | |
30 | Alexandrium pseudogonyaulax, cell size | A. pseudogonyaulax cell size | μm | Möller, Kristof | Visual identification (VID) of Lugol preserved samples | |
31 | Alexandrium pseudogonyaulax, strain | A. pseudogonyaulax strain | Möller, Kristof | |||
32 | Comment | Comment | Möller, Kristof |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3398 data points
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