Cardoso, Isabel; Meiβner, Anneke; Hofmann, Laurie C (2024): The effect of light intensity versus light dose on the antioxidant activity of two strains of Ulva lacinulata [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.965065
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Published: 2024-01-25 • DOI registered: 2024-07-30
Abstract:
The genus Ulva, described as a good source of antioxidants known for its antibacterial properties and associated with the capacity to adapt to different environments and high growth rates has justified the increasing interest in its large-scale production. . While extensive research has been done on optimizing the extraction of Ulva's bioactive compounds, few studies were conducted on increasing or optimizing antioxidant activity of Ulva spp. during cultivation. Our study aimed to investigate an energy-saving cultivation method for optimizing Ulva lacinulata by testing the impact of light dose and light intensity on its antioxidant activity. Two geographically different strains (NE-Atlantic and Mediterranean) were observed for 5 days under two light intensities (70 or 185 µmol photons m-2 s-1) with the same light dose (4 mol photons m-2 d-1). Samples were collected at different times to evaluate the variation of antioxidant activity (using the ABTS radical decolorisation assay). A strain-dependent response was observed between the NE-Atlantic strain which had significantly higher antioxidant activity after 5 days under the saturating-light, while the Mediterranean strain was not significantly impacted, suggesting that light dose may significantly affect antioxidant activity in certain Ulva spp.
Keyword(s):
Related to:
Cardoso, Isabel; Meiβner, Anneke; Hofmann, Laurie C (2024): The effect of irradiance versus light dose on the antioxidant activity of two strains of Ulva lacinulata. Botanica Marina, 0(0), https://doi.org/10.1515/bot-2023-0064
Funding:
Federal Ministry of Food and Agriculture (BMEL), grant/award no. 281DL02B20: Verbundprojekt: Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel (MakPakScaleUp) - Teilprojekt B
Coverage:
Median Latitude: 39.980497 * Median Longitude: 6.872087 * South-bound Latitude: 39.394977 * West-bound Longitude: -9.213583 * North-bound Latitude: 40.566018 * East-bound Longitude: 22.957756
Date/Time Start: 2017-01-01T00:00:00 * Date/Time End: 2021-01-04T12:00:00
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 0 m
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Hofmann, Laurie C | |||
| 2 | Latitude of event | Latitude | Hofmann, Laurie C | |||
| 3 | Longitude of event | Longitude | Hofmann, Laurie C | |||
| 4 | Date/Time of event | Date/Time | Hofmann, Laurie C | |||
| 5 | DEPTH, water | Depth water | m | Hofmann, Laurie C | Geocode | |
| 6 | Type of study | Study type | Hofmann, Laurie C | |||
| 7 | Laboratory | Lab | Hofmann, Laurie C | |||
| 8 | Latitude 2 | Latitude 2 | Hofmann, Laurie C | of laboratory | ||
| 9 | Longitude 2 | Longitude 2 | Hofmann, Laurie C | of laboratory | ||
| 10 | Date/time start, experiment | Date/time start exp | Hofmann, Laurie C | |||
| 11 | Date/time end, experiment | Date/time end exp | Hofmann, Laurie C | |||
| 12 | Species, unique identification | Species UID | Hofmann, Laurie C | |||
| 13 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Hofmann, Laurie C | |||
| 14 | Species, unique identification (URI) | Species UID (URI) | Hofmann, Laurie C | |||
| 15 | Collection | Collection | Hofmann, Laurie C | Collection number | ||
| 16 | Accession number, genetics | Access no gen | Steinhagen, Sophie | Molecular analysis with the Invisorb Spin Plant Mini Kit (Stratec); gel electrophoresis and subsequently purified using the QIAquick PCR Purification Kit (Qiagen); Sanger sequencing was performed by Eurofins Genomics (Konstanz, Germany); Sequence reads were assembled in sequencher (version 4.1.4, Gene Codes Corporation). BLAST function in GenBank, identifications via the specimens' tufA sequences were made. | ||
| 17 | Experiment duration | Exp duration | h | Hofmann, Laurie C | ||
| 18 | Replicate | Repl | Hofmann, Laurie C | |||
| 19 | Sample, dry mass | Samp dm | g | Hofmann, Laurie C | Precision scale, Sartorius | |
| 20 | Treatment: temperature | T:temp | °C | Hofmann, Laurie C | Digital thermometer | |
| 21 | Treatment: light:dark cycle | T:L:D | hh:hh | Hofmann, Laurie C | Hours of light and dark exposure | |
| 22 | Treatment: light condition | T:light | Hofmann, Laurie C | "SL" - Saturating Light, "C" - Control | ||
| 23 | Treatment: light intensity | T:Io | µmol/m2/s | Hofmann, Laurie C | Light meter, LI-COR Biosciences GmbH, LI-250A | |
| 24 | Treatment: nutrients | T:nutrients | Hofmann, Laurie C | Fertilizer: Blaukorn (COMPO SANA®, Germany) | ||
| 25 | Treatment: nutrients | T:nutrients | Hofmann, Laurie C | Concentration in ml/l | ||
| 26 | Antioxidant capacity, in Trolox Equivalents | TEAC | µg/ml | Hofmann, Laurie C | Microplate reader, Tecan Trading AG, Infinite 200 | A Trolox standard curve was created by measuring the absorption of different Trolox concentrations (0 - 100 µg mL-1) in ethanol after being mixed with ABTS. The Trolox calibration curve was then used to calculate the AA of the samples in Trolox equivalents TE in µg mL-1. |
Change history:
2024-09-06T09:28:02 – The sampling date of Ulva in NE Atlantic was corrected to 2021-01-04. Original (wrong) date was 2021-04-01.
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1140 data points