Matthiessen, Birte; Hattich, Giannina S I; Pulina, Silvia; Hansen, Thomas; Reusch, Thorsten B H; Hamer, Jorin (2023): Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: genotyping [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.957916, In: Matthiessen, B et al. (2023): Experiments on coexisting phytoplankton species Chaetoceros affinis and Emiliania huxleyi in response to nutrients [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.957930
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Abstract:
Measurements of genotype composition in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. The genotype composition was assessed for each species after batch cycles 7,13, and 26 by using microsatellites.
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# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
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1 | Type of study | Study type | Matthiessen, Birte | |||
2 | Sampling date/time, experiment | Date/time sampling exp | Matthiessen, Birte | |||
3 | Bottle number | Bottle | # | Matthiessen, Birte | ||
4 | Sample ID | Sample ID | Matthiessen, Birte | |||
5 | Batch | Batch | Matthiessen, Birte | |||
6 | Species, unique identification | Species UID | Matthiessen, Birte | |||
7 | Species, unique identification (URI) | Species UID (URI) | Matthiessen, Birte | |||
8 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Matthiessen, Birte | |||
9 | Treatment: nutrients | T:nutrients | Matthiessen, Birte | Nutrient regime | ||
10 | Genotype | Genotype | Matthiessen, Birte | Genotyping using microsatellites | First Identified Genotype | |
11 | Genotype | Genotype | Matthiessen, Birte | Genotyping using microsatellites | Second Identified Genotype | |
12 | Genotype | Genotype | Matthiessen, Birte | Genotyping using microsatellites | Third Identified Genotype |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
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Curation Level: Enhanced curation (CurationLevelC)
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3852 data points
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