Citation:

PANGAEA.957930

Name: Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: genotyping
License: https://creativecommons.org/licenses/by/4.0/
Keywords: cell size; diatoms; evolution; Genotype Sorting; Laboratory experiment; nutrients; phenotypic plasticity; Phytoplankton; Species Sorting; trait variability

Facebook Twitter

Abstract:

Measurements of genotype composition in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. The genotype composition was assessed for each species after batch cycles 7,13, and 26 by using microsatellites.

Keyword(s):

cell size; diatoms; evolution; Genotype Sorting; Laboratory experiment; nutrients; phenotypic plasticity; Phytoplankton; Species Sorting; trait variability

Project(s):

Priority Programme 1704 DynaTrait: Flexibility Matters: Interplay between Trait Diversity and Ecological Dynamics Using Aquatic Communities as Model Systems (SPP1704)

Funding:

German Research Foundation (DFG), grant/award no. MA5058/2-2

German Research Foundation (DFG), grant/award no. RE1708/17-2

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Matthiessen, Birte
2	Sampling date/time, experiment	Date/time sampling exp		Matthiessen, Birte
3	Bottle number	Bottle	#	Matthiessen, Birte
4	Sample ID	Sample ID		Matthiessen, Birte
5	Batch	Batch		Matthiessen, Birte
6	Species, unique identification	Species UID		Matthiessen, Birte
7	Species, unique identification (URI)	Species UID (URI)		Matthiessen, Birte
8	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Matthiessen, Birte
9	Treatment: nutrients	T:nutrients		Matthiessen, Birte		Nutrient regime
10	Genotype	Genotype		Matthiessen, Birte	Genotyping using microsatellites	First Identified Genotype
11	Genotype	Genotype		Matthiessen, Birte	Genotyping using microsatellites	Second Identified Genotype
12	Genotype	Genotype		Matthiessen, Birte	Genotyping using microsatellites	Third Identified Genotype

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3852 data points

Download Data (login required; moratorium until 2025-04-06)

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML (shows only first 2000 rows)